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Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Fig. 2 | Clinical Phytoscience

Fig. 2

From: Neuroprotective effects of Peltophorum pterocarpum leaf extract against hydrogen peroxide induced oxidative stress and cytotoxicity

Fig. 2

Images of IMR32 cells. a Phase contrast image of undifferentiated human neuroblastoma IMR32 cells. Its shows proliferating cells. The red color arrows indicate fibroblast type cells and green. Color arrow indicate neuroblast type cells. b 2.5 μM Brdu differentiated human IMR32 cells (Div15 days). It shows neuron like properties including neuritic outgrowth and morphological changes. Red arrows shows the elongated neurites, green arrows connected clumps of cells. c ICC image of the expression of class III Beta-tubulin neuron marker in Div15 dIMR32 cells. Nuclei stained in Propidium iodide (PI red) and post mitotic neurons staining with class III β-tubulin (green). d Expression of ChAT (Choline Acetyltransferase) cholinergic neuron cell marker in dIMR32 cells. Nuclei stained with DAPI (blue) and cholinergic neurons stained with ChAT (red)

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