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Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Fig. 8 | Clinical Phytoscience

Fig. 8

From: Neuroprotective effects of Peltophorum pterocarpum leaf extract against hydrogen peroxide induced oxidative stress and cytotoxicity

Fig. 8

a, b c shows the western blot bands of p38, ERK1/2 and JNK respectively. d Effect of PTE pre-treatment (24 h) prior to 2 h H2O2 treatment on the expression of phospho-p38 in dIMR32 cells. e Effect of PTE pre-treatment (24 h) prior to 2 h H2O2 treatment on the expression of phospho-ERK1/2 in dIMR32 cells. f Effect of PTE pre-treatment (24 h) prior to 2 h H2O2 treatment on the expression of phospho- JNK in dIMR32 cells. The ratio between density of the bands between phospho-p38/total p- 38 were calculated (Arbitary Unit). The data are expressed as mean ± SEM, n = 3. The label UT = untreated control cells. Statistical significance was analyzed with one-way ANOVA followed by Dunnett’s multiple comparison post host test. The western blot density of each protein band was quantified using the ImageJ software. There was significant difference between treatment ** p < 0.05 and ****p < 0.0001. UT = untreated control. PTE 250 = PTE pre-treatment (24 h) at 250 μg/ml and PTE 125 = PTE pretreatment (24 h) at 125 μg/ml. Beta actin was used as loading control

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