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International Journal of Phytomedicine and Phytotherapy

Table 2 Effect of two different cytokinins supplemented with MS basal medium and culture duration (i.e., regeneration cycles) on adventitious shoot induction from leaf explant of Bacopa monnieri

From: Improved micropropagation of Bacopa monnieri (L.) Wettst. (Plantaginaceae) and antimicrobial activity of in vitro and ex vitro raised plants against multidrug-resistant clinical isolates of urinary tract infecting (UTI) and respiratory tract infecting (RTI) bacteria

Concentration of plant growth regulators Response (%) Number of shoot buds induced per leaf explant (after 12 d of implantation) No. of elongated (≥ 1 cm) shoot buds per leaf explant (after 42 d of implantation)
BAP (mg/L) KIN (mg/L) Spermidine (mM)
0 0a 0a
0.5 96.7 53.5 ± 1.7cd 11.7 ± 0.3c
1.0 100 76.7 ± 1.2de 16.1 ± 0.2d
1.5 100 92.4 ± 3.7ef 22.5 ± 0.4ef
2.0 100 84.1 ± 3.2e 19.2 ± 0.5e
2.5 100 78.2 ± 2.4e 13.8 ± 0.2cd
0.5 90.0 45.0 ± 1.3bcd 9.5 ± 0.6c
1.0 93.3 62.8 ± 1.9d 13.6 ± 0.7cd
1.5 100 73.5 ± 2.2de 16.0 ± 0.3d
2.0 100 81.3 ± 2.5e 18.2 ± 1.0de
2.5 100 75.4 ± 1.9def 15.4 ± 0.8d
1.5 1.0 100 106.0 ± 3.2f 27.3 ± 0.7fg
1.5 1.5 100 114.2 ± 2.6fg 30.7 ± 0.9g
1.5 2.0 100 123.8 ± 3.4g 34.9 ± 1.2h
1.5 2.5 100 117.6 ± 3.1fg 31.4 ± 1.1gh
  1. Each value represents the mean ± standard error, n = 30 (3 set, 10 samples in each set). Mean followed by the same letters in each column are not significantly different at P < 0.05 according to Tukey’s multiple range test. BAP 6-Benzylaminopurine, KIN Kinetin