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International Journal of Phytomedicine and Phytotherapy

Table 2 Effect of two different cytokinins supplemented with MS basal medium and culture duration (i.e., regeneration cycles) on adventitious shoot induction from leaf explant of Bacopa monnieri

From: Improved micropropagation of Bacopa monnieri (L.) Wettst. (Plantaginaceae) and antimicrobial activity of in vitro and ex vitro raised plants against multidrug-resistant clinical isolates of urinary tract infecting (UTI) and respiratory tract infecting (RTI) bacteria

Concentration of plant growth regulators

Response (%)

Number of shoot buds induced per leaf explant (after 12 d of implantation)

No. of elongated (≥ 1 cm) shoot buds per leaf explant (after 42 d of implantation)

BAP (mg/L)

KIN (mg/L)

Spermidine (mM)

–

–

–

0

0a

0a

0.5

–

–

96.7

53.5 ± 1.7cd

11.7 ± 0.3c

1.0

–

–

100

76.7 ± 1.2de

16.1 ± 0.2d

1.5

–

–

100

92.4 ± 3.7ef

22.5 ± 0.4ef

2.0

–

–

100

84.1 ± 3.2e

19.2 ± 0.5e

2.5

–

–

100

78.2 ± 2.4e

13.8 ± 0.2cd

–

0.5

–

90.0

45.0 ± 1.3bcd

9.5 ± 0.6c

–

1.0

–

93.3

62.8 ± 1.9d

13.6 ± 0.7cd

–

1.5

–

100

73.5 ± 2.2de

16.0 ± 0.3d

–

2.0

–

100

81.3 ± 2.5e

18.2 ± 1.0de

–

2.5

–

100

75.4 ± 1.9def

15.4 ± 0.8d

1.5

–

1.0

100

106.0 ± 3.2f

27.3 ± 0.7fg

1.5

–

1.5

100

114.2 ± 2.6fg

30.7 ± 0.9g

1.5

–

2.0

100

123.8 ± 3.4g

34.9 ± 1.2h

1.5

–

2.5

100

117.6 ± 3.1fg

31.4 ± 1.1gh

  1. Each value represents the mean ± standard error, n = 30 (3 set, 10 samples in each set). Mean followed by the same letters in each column are not significantly different at P < 0.05 according to Tukey’s multiple range test. BAP 6-Benzylaminopurine, KIN Kinetin