Phytochemical studies, antiangiogenic, anti-inflammatory and antioxidant activities of Scyphocephalium ochocoa Warb. (Myristicaceae), medicinal plant from Gabon

Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Table 5 Effect of S. ochocoa extracts and diclofenac sodium against membrane stabilization

	Conc. (μg/mL)	% Inhibition of extracts from S. ochocoa			Sodium diclofenac
	Conc. (μg/mL)	Water	Water-ethanol	Ethanol	% Inhibition
Hemolysis induced by hypotonicity	250	66.144 ± 1.663	56.867 ± 1.948	76.348 ± 0.017	100.000 ± 0.000
	125	56.532 ± 0.744	50.626 ± 0.452	51.937 ± 0.296	100.000 ± 0.000
	62.5	53.436 ± 0.603	43.873 ± 0.152	30.573 ± 0.163	99.583 ± 1.078
	31.25	32.633 ± 0.452	21.256 ± 1.285	14.720 ± 0.311	99.132 ± 1.068
	15.625	10.874 ± 0.149	10.539 ± 1.095	7.672 ± 1.976	0.885 ± 0.121
	IC₅₀ (μg/mL)	56.713 ± 0.492	71.200 ± 1.833	133.375 ± 0.661	58.590 ± 1.021
	P value	0.471		0.016	0.471
Hemolysis induced by heat	250	46.850 ± 0.059	56.867 ± 2.948	69.822 ± 2.791	71.039 ± 0.112
	125	57.261 ± 0.102	41.033 ± 1.826	56.118 ± 3.369	32.971 ± 0.640
	62.5	56.985 ± 0.120	33.077 ± 1.442	35.542 ± 2.841	13.788 ± 3.527
	31.25	33,639 ± 0.137	20.704 ± 2.170	12.777 ± 1.437	6.430 ± 1.453
	15.625	7.710 ± 0.120	1.667 ± 0.995	7.553 ± 1.878	2.911 ± 0.147
	IC₅₀ (μg/mL)	36.793 ± 0.529	39.526 ± 0.710	48.912 ± 0.9573	180.911 ± 2.205
	P value	0.273			0.001

For hypotonicity-induced hemolysis, the ability to stabilize the membrane is similar for water extract, ethanol and diclofenac (p value = 0.471). A significant difference is observed with ethanol extract (p value = 0.016)
For heat-induced hemolysis, all extracts have the same effect on membrane stabilization (p value = 0.273). However, their effect is significantly different from that of diclofenac (p value = 0.001)