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International Journal of Phytomedicine and Phytotherapy

Table 1 Phytochemical constituents identified in the methanol and petroleum ether extracts of C. renigera seed extracts

From: Evaluation of bioactivities of methanol and petroleum ether extracts of Cassia renigera seed

Phytochemicals

Name of the test

Observed changes

Result

CRSM

CRSP

Alkaloids

Mayer’s test

Creamy white precipitate

+

–

Hager’s test

Yellow crystalline precipitate

++

+

Wagner’s test

Brown or deep brown precipitate

+

–

Dragendorff’s test

Orange or orange-red precipitate

+

–

Carbohydrates

Molisch’s test (General test for Carbohydrates)

A red or reddish violet ring is formed at the junction of two layers and on shaking a dark purple solution is formed

++

+/−

Barfoed’s test (General test for Monosaccharides)

Red precipitate

–

–

Fehling’s test(for reducing sugar)

A red or brick-red precipitate

+

+

Test for combined Reducing Sugar

A brick-red precipitate

+

+/−

Glycosides

General test

Yellow color

+

–

Test for Glucoside

Production of brick-red precipitation (carried out with the hydrolyzed extract)

+

–

Flavonoids

General test

Red color

+

+

Saponins

Frothing test

Formation of stable foam

+

+

Steroids

Libermann-Burchard’s test

Greenish color

+

–

Tannins

Lead acetate test

A yellow or red precipitate

+

+

  1. [++ = Strong presence, + = Presence, − = Absence, +/− = Presence/Absence not confirmed]; CRSM = Cassia renigera seed methanol extract, CRSP = Cassia renigera seed petroleum ether extract