Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

Ghosh, Debjit; Mondal, Sumanta; Ramakrishna, K.

doi:10.1186/s40816-019-0106-2

Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Table 2 Effects of ARELE on haematological parameters in both male and female rats treated for 30 days

From: Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote

Sex	Parameter	Unit	Control	ARELE (mg/kg, b.wt.)
Sex	Parameter	Unit	Control	125	250	500
Male
Haemoglobin		gms/dL	13.64 ± 0.53	14.45 ± 0.69	12.58 ± 1.62	11.87 ± 1.04*
WBC		10³/μL	8.89 ± 2.22	9.46 ± 0.95	11.86 ± 1.89*	11.42 ± 1.94
RBC		10⁶/μL	8.22 ± 0.45	8.31 ± 0.61	8.33 ± 0.41	8.765 ± 0.21
Haematocrit (HCT)		%	38.33 ± 4.92	40.41 ± 4.17	45.83 ± 6.45*	46.17 ± 4.15*
Platelets (PLT)		10⁵/mm³	7.87 ± 0.66	7.83 ± 0.21	8.033 ± 0.36	8.27 ± 0.49
Mean corpuscular volume (MCV)		fL	52.46 ± 2.71	50.38 ± 2.80	50.28 ± 3.49	52.99 ± 2.53
Mean corpuscular haemoglobin (MCH)		pg	17.73 ± 0.91	17.95 ± 0.88	17.36 ± 0.46	17.93 ± 0.74
Mean corpuscular hemoglobin concentration (MCHC)		%	29.11 ± 1.81	29.01 ± 2.48	29.2 ± 2.87	26.4 ± 2.51
Differential leucocyte count
Neutrophils		%	20.5 ± 6.92	18.67 ± 5.82	24.33 ± 5.82	23.83 ± 4.02
Lymphocytes		%	73.67 ± 7.09	72.5 ± 6.09	74 ± 6.13	73.16 ± 3.97
Eosinophils		%	1.5 ± 0.83	2.33 ± 1.03	4.67 ± 1.51**	4.33 ± 0.81**
Monocytes		%	2.83 ± 1.33	2.5 ± 1.05	2.17 ± 1.17	2.66 ± 1.75
Female
Haemoglobin		gms/dL	13.66 ± 0.86	13.41 ± 0.61	13.80 ± 0.71	14.13 ± 0.68
WBC		10³/μL	8.19 ± 1.78	8.25 ± 1.13	7.90 ± 1.35	7.17 ± 1.18
RBC		10⁶/μL	7.34 ± 0.70	7.39 ± 0.56	7.30 ± 0.51	7.99 ± 0.44
Haematocrit (HCT)		%	41.51 ± 2.74	41.19 ± 2.72	39.96 ± 2.92	43.49 ± 1.76
Platelets (PLT)		10⁵/mm³	7.54 ± 0.45	6.065 ± 0.52**	4.34 ± 0.47**	3.055 ± 0.35**
Mean corpuscular volume (MCV)		fL	53.18 ± 3.71	53.39 ± 3.57	54.16 ± 4.11	58.64 ± 2.79*
Mean corpuscular haemoglobin (MCH)		pg	18.08 ± 1.40	19.36 ± 1.17	18.51 ± 1.88	20.61 ± 1.31*
Mean corpuscular hemoglobin concentration (MCHC)		%	33.72 ± 2.11	32.21 ± 2.31	32.18 ± 1.70	34.15 ± 1.58
Differential leucocyte count
Neutrophils		%	20.93 ± 4.33	32.51 ± 3.04**	37.93 ± 5.43**	51.21 ± 4.47**
Lymphocytes		%	71.82 ± 5.76	53.75 ± 5.62**	49.5 ± 4.31**	41.46 ± 4.79**
Eosinophils		%	1.64 ± 0.77	3.13 ± 1.15	2.65 ± 1.36	4.5 ± 0.77**
Monocytes		%	2.61 ± 0.55	2.50 ± 0.66	2.76 ± 0.30	2.57 ± 0.48

Values are expressed as mean ± S.D. (n = 6). Statistical analysis done by one-way ANOVA followed by Dunnet’s t-test
**P < 0.01 compared to control group
*P < 0.05 compared to control group

Back to article page