International Journal of Phytomedicine and Phytotherapy
Table 1 Observation and results of phytochemical screening
Secondary metabolite | Name of the test | Observation | Result |
|---|---|---|---|
Alkaloids | 1. Mayer’s test | White or creamy white precipitate | +++ |
2. Hager’s test | Yellow crystalline precipitate | +++ | |
3. Wagner’s test | Brown or deep brown precipitate | +++ | |
Glycosides | General test | No yellow color | – |
Cardiac glycosides | 1. Legal’s test | No pink or red color | – |
2. Baljet’s test | No yellow orange color | – | |
Phenolics | FeCl3 test | Violet color | +++ |
Terpenoids | Salkowsky test | Reddish color | +++ |
Carbohydrates | 1. Molisch Test | Red-violet layer at the interface between the acid (bottom) and aqueous (upper) layers | +++ |
2. Fehling’s Test | Red precipitate | +++ | |
Flavonoids | 1. General test | A yellow colouration | +++ |
2. Specific test | Orange to red color | +++ | |
Steroids | Libermann-Burchard’s test | Greenish color | +++ |
Tannins | FeCl3 test | No brownish green color | – |
Saponins | Frothing test | Change is observed | +++ |
Phlobatanins | General test | No Red precipitate formation | – |