In-vivo and in-vitro evaluation of pharmacological activities of Ardisia solanacea leaf extract

Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Table 2 Effect of methanolic extract of Ardisia solanacea on EPM test apparatus while test session

Test Groups	Time spent in open arm (Sec) (Mean ± SEM)	Number entries into Open arm (Mean ± SEM)	Time spent in closed arm (Sec) (Mean ± SEM)	Number entries into closed arm (Mean ± SEM)
Control	60.00 ± 3.35	7.00 ± 0.55	240.00 ± 3.35	18.00 ± 1.09
Diazepam (Standard)	230.00 ± 5.04^a	18 ± 1.30^a	70.00 ± 3.89^a	8.00 ± 1.30^a
ASME 200	25.34 ± 0.88^a	4.34 ± 0.42	273.00 ± 2.16^a	12.83 ± 1.30^a
ASME 400	23.00 ± 1.05^a	3.40 ± 0.25^a	277.00 ± 1.04	16.20 ± 0.37^a
ASNH 200	24.75 ± 4.76^a	4.25 ± 0.63	275.25 ± 4.76^a	12.75 ± 0.85^a
ASNH 400	18.20 ± 1.36^a	4.60 ± 1.36	281.80 ± 1.36^a	5.2 ± 0.58^a
ASCF 200	271.40 ± 2.29^a	11.60 ± 0.51^a	28.60 ± 2.29^a	5.80 ± 0.58^a
ASCF 400	278.20 ± 1.39^a	15.00 ± 0.70^a	21.80 ± 1.39^a	4.20 ± 0.37^a
ASCC 200	66.20 ± 1.77	7.00 ± 0.70	190.00 ± 1.52	21.20 ± 1.02^a
ASCC 400	43.40 ± 1.21^a	6.00 ± 1.58	236.40 ± 1.36^a	23.60 ± 0.93

Note: Each value represents the mean ± SEM. (n = 5). One- way ANOVA followed by Dunnett’s t test. *p < 0.05, ##p < 0.005 compared with control. Where “a” represents the test was significant. ASME = Methanolic Extract of Ardisia solanacea, ASNH = n-hexane soluble fraction, ASCF = Chloroform soluble fraction, ASCC = Carbon-tetrachloride soluble fraction