In vivo and in silico evaluation of analgesic activity of Lippia alba

Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Table 3 Effects of different extracts of L. alba in hot plate test

Treatment group	Dose	n	Latency Time (s)
Treatment group	Dose	n	0 min	+ 30 min	+ 60 min	+ 90 min
Control	10 ml/kg	5	6.67 ± 0.08	6.90 ± 0.38	9.77 ± 0.40	9.18 ± 0.16
Morphine	5 mg/kg	5	8.52 ± 0.08*	10.50 ± 0.42*	11.99 ± 0.31*	13.96 ± 0.10*
ME	250 mg/kg	5	6.35 ± 0.14	8.36 ± 0.31	10.58 ± 0.98	12.09 ± 0.26*
ME	500 mg/kg	5	6.91 ± 0.10	9.36 ± 0.38*	10.49 ± 0.56	13.13 ± 0.53*
PEE	250 mg/kg	5	6.90 ± 0.10	8.29 ± 0.49	10.42 ± 0.38	10.94 ± 0.58*
PEE	500 mg/kg	5	7.20 ± 0.14	9.31 ± 0.19*	11.86 ± 0.26*	13.25 ± 0.45*
DCME	250 mg/kg	5	5.53 ± 0.34	7.71 ± 0.42	10.77 ± 0.29	13.16 ± 0.51*
DCME	500 mg/kg	5	5.84 ± 0.50	9.41 ± 0.20*	12.00 ± 0.17*	13.48 ± 0.33*

Latency time values are present as mean ± standard error of mean. 0 min means 30 min before drug administration, + 30 min, + 60 min and + 90 min indicate 30, 60 and 90 min after drug and extract administration, respectively. Tests of within-subjects effects reveal that for the factor ‘Time’ calculated F = 38.472 for all methods and P value = 0.000 in every case. So time is highly significant at any level of significance. *P < 0.05, vs control. Repeated measure analysis of variance with Dunnett’s multiple comparison was performed to analyze this data set. ME: methanolic extract; PEE: petroleum ether extract; DCME: dichloromethane extract