Evaluation of antioxidant and anti-inflammatory potency of Lepidium pinnatifidum Ledeb

Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Table 4 Effect of L. pinnatifidum on heat induced protein denaturation at 600 nm

Treatment	%inhibition of protein denaturation					IC₅₀ (μg/ ml
	500 μg /ml	400 μg /ml	300 μg /ml	200 μg /ml	100 μg /ml
Dic. Pot.	0.13 ± 0.01 84.70 ± 1.17^a	0.27 ± 0.01 68.23 ± 1.17^a	0.39 ± 0.01 54.11 ± 1.17^a	0.45 ± 0.01 47.05 ± 1.17^a	0.51 ± 0.01 40 ± 1.17^a	186.3
LPM	0.33 ± 0.01 61.17 ± 1.17^d	0.49 ± 0.01 42.35 ± 1.14^d	0.54 ± 0.01 36.47 ± 1.16^d	0.59 ± 0.01 30.58 ± 1.17^d	0.66 ± 0.01 22.35 ± 1.17^d	436.0
LPH	0.21 ± 0.01 75.29 ± 1.17^b	0.35 ± 0.01 58.82 ± 1.17^b	0.42 ± 0.01 50.58 ± 1.16^b	0.49 ± 0.01 42.35 ± 1.16^b	0.55 ± 0.01 35.29 ± 1.15^b	237.7
LPC	0.41 ± 0.01 51.76 ± 1.17^e	0.54 ± 0.01 36.47 ± 1.17^e	0.59 ± 0.01 30.58 ± 1.17^e	0.65 ± 0.01 23.52 ± 1.16^e	0.71 ± 0.01 16.47 ± 1.15^e	567.5
LPE	0.27 ± 0.01 68.23 ± 1.17^c	0.43 ± 0.01 49.41 ± 1.17^c	0.47 ± 0.01 44.70 ± 1.17^c	0.56 ± 0.01 34.11 ± 1.17^c	0.62 ± 0.01 27.05 ± 1.16^c	333.3
LPB	0.46 ± 0.01 45.88 ± 1.16^f	0.58 ± 0.01 31.76 ± 1.17^f	0.64 ± 0.01 24.70 ± 1.16^f	0.7 ± 0.01 17.64 ± 1.17^f	0.77 ± 0.01 9.4 ± 1.17^f	621.3
LPA	0.48 ± 0.01 43.52 ± 1.17^f	0.62 ± 0.01 27.05 ± 1.16^g	0.68 ± −0.01 20 ± 1.16^g	0.72 ± 0.01 15.29 ± 1.15^f	0.82 ± 0.01 3.5 ± 1.17^g	625.5

values shown represent Mean ± SD and percentage inhibition at different concentrations; (n = 3). Different superscript (^a-g) indicate significance at P < 0.05. LPM: L. pinnatifidum Methanol extract. LPH: L. pinnatifidum n-Hexane fraction. LPC: L. pinnatifidum Chloroform fraction. LPE: L. pinnatifidum Ethyl acetate fraction. LPB: L. pinnatifidum Butanol fraction. LPA: L. pinnatifidum Aqueous fraction