Evaluation of antioxidant, anti-hemolytic, cytotoxic effects and anti-bacterial activity of selected mangrove plants (Bruguiera gymnorrhiza and Heritiera littoralis) in Bangladesh

Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Table 2 Anti-hemolytic activity of methanol leaf extracts of selected mangrove plant species

Tested materials	Positive control (A)	Negative control (B)	Optical density at 540 nm at concentration (μg/mL)				IC₅₀ (μg/mL)
Tested materials	Positive control (A)	Negative control (B)	125	250	500	1000	IC₅₀ (μg/mL)
B. gymnorriza	1.45 ± 0.005	0.11 ± 0.001	0.72 ± 0.01	0.82 ± 0.01	0.94 ± 0.01	1.18 ± 0.01	311.29 ± 5.48^**
H. littoralis	1.45 ± 0.005	0.11 ± 0.001	0.61 ± 0.01	0.72 ± 0.02	0.88 ± 0.02	1.02 ± 0.01	526.90 ± 25.85^**
L-ascorbic acid	1.45 ± 0.005	0.11 ± 0.001	0.71 ± 0.02	0.76 ± 0.01	1.08 ± 0.01	1.41 ± 0.01	282.14 ± 6.96^**

All values represent mean ± SEM (n = 4), Statistical significance (p < 0.005) is the same for values in the same column (^**). A; phosphate buffer saline treatment results in 0% hemolysis, B; hydrogen peroxide replaced extract to serve as a negative control, treatment results with 100% hemolysis