International Journal of Phytomedicine and Phytotherapy
Table 2 In vitro anti-diabetic activity of medicinal plants in Ethiopia
Study | Scientific name | Parts used | Extraction method | Assay method | Active cpd, Fraction / extraction | IC50 |
|---|---|---|---|---|---|---|
Alema et al. 2020 [10] | Terminalia brownii Fresen | stem bark | methanolic extract & solvent fractions | α-Amylase Inhibition (chromogenic DNSA method) | aqueous fraction | > 100 μg/ml |
Butanol fraction | 84.69 μg/ml | |||||
chloroform fraction | 63.41 μg/ml | |||||
ethyl acetate fraction | > 100 μg/ml | |||||
crude extract | > 100 μg/ml | |||||
Acarbose | ~ 12.5 μg/ml | |||||
Kifle and Eneyew., 2020 [34] | Bersama abyssinica | leaf | Methanolic Extraction | α-Amylase Inhibition (chromogenic DNSA method) | Chloroform fraction | 30.97 + 0.84 |
Ethyl acetate fraction | 20.34 + 0.67 | |||||
Aqueous fraction | 13.33 + 0.57 | |||||
Crude extract | 6.57 + 0.74 | |||||
Acarbose | 2.26 + 0.53 | |||||
Toma et al. 2014 [8] | Moringa Stenopetala | powdered leaves | ethanol extract | Pancreatic α-amylase | ethanol extract | > 5 mg/ml |
Maltase | > 5 mg/ml | |||||
Pancreatic C. esterase | > 5 mg/ml | |||||
Pancreatic lipase | > 5 mg/ml | |||||
Sucrase | 1.47 ± 0.19 mg/ml | |||||
Tekulu et al. 2019 [25] | Aloe megalacantha B Aloe monticola R | leaf | Methanol extract | α-Amylase Inhibition (chromogenic DNSA method) | TLC fraction from A. megalacantha coded as AM1 | 37.83 ± 3.31 μg/mL |
Leaf latex ofA. megalacantha | 74.76 ± 1.98 μg/mL | |||||
TLC fraction from A. megalacantha coded as AM2 | 96.75 ± 1.98 μg/mL | |||||
Leaf latex ofA. monticola | 78.10 ± 1.88 μg/mL | |||||
TLC fraction from A. monticola, AG1 | 56.95 ± 1.88 μg/mL | |||||
TLC fraction from A. monticola, AG2 | 64.03 ± 3.60 μg/mL | |||||
Acarbose | 16.49 ± 1.91 μg/mL |