Table 3 Experimental Design

I

Normal control received only the vehicle (1 ml/kg olive oil twice a week for 8 weeks)

II

Received Ethanol (50% v/v) daily for 8 weeks

III

Treatment with ethanol (50% v/v) for 4 weeks and then pre-treated with herbal formulation AKSS16-LIV01(75 mg/kg bw/day) for next 4 weeks

IV

Treatment with ethanol (50% v/v) for 4 weeks and then pre-treated with herbal formulation AKSS16-LIV01(150 mg/kg bw/day) for next 4 weeks

V

Treatment with ethanol (50% v/v) for 4 weeks and then pre-treated with herbal formulation AKSS16-LIV01(300 mg/kg bw/day) for next 4 weeks

VI

Treatment with ethanol (50% v/v) for 4 weeks and then pre-treated with Silymarin standard hepatoprotective drug at a dose (100 mg/kg bw) for next 4 weeks

VII

Treated with multi herbal formulation (MHF) AKSS16-LIV01 (300 mg/kg bw/day all over the experiment).