Fig. 4

Representative photomicrographs of cardiac tissue sections of rat groups stained with H&E. (× 200). (A,a): Normal control group showing typical normal pattern of cardiac muscle fibers (arrow) and normal vascular structures (bi-forked thick arrow). (B,b): ISO group showing focal atrophic, apoptotic, degenerative and necrotic changes in some muscle fibers (light head arrow). Interstitial and perivascular edematous reactions, fatty degeneration and cardiomyolysis are also seen (arrow& curved thick arrow). (C,c): LY294002 + ISO group showing focal atrophic, degenerative and apoptotic changes in some cardiomyocytes (light-head arrow), beside focal interstitial and perivascular edematous reaction (thick arrow) with degenerative changes in some vascular walls (bi-forked thick arrow). Normal heart muscles, intercalated discs and sarcolemmal cells are also seen (bent arrow). (D,d): DATS+ISO group showing most of the cardiomyocytes and the sarcolemmal cells were apparently normal (arrow), along with mild focal cardiomyocytic atrophic and degenerative changes (light-head arrow), and a few cardiomyocytes shows fatty degenerative change (curved arrow). (E,e): CAP+ISO group showing normal cardiomyocytes, sarcolemmal cells with interstitial tissue (bent arrow). Mild cardiomyocytic degenerative, apoptotic changes and focal intestinal edematous inflammatory process (light-head arrow & thick arrow respectively). (F,f): DATS+LY294002 + ISO group showing marked histopathologic changes represented by cardiomyocytic atrophic, degenerative and apoptotic changes (light-head arrow), with a moderate interstitial edematous inflammatory reaction (thick arrow). Other cardiac muscles and structures were apparently normal (arrow). (G,g): CAP+LY294002 + ISO group showing cardiomyocytes suffered atrophic, degenerative and apoptotic changes (thick arrow) and a few cells with fatty degeneration (curved arrow). Other cardiac muscles and structures are apparently normal (light-head arrow). A,B,C,D,E,F,G; scale bars 100um. a,b,c,d,e,f,g; scale bars 50um