Analgesic, anti-inflammatory and NF-κB inhibitory activity of aerial parts of Cestrum diurnum

Cestrum diurnum L. (Solanaceae), locally known as buno-Hasnahena, is widely used in different traditional medicinal practices to treat pain, burn, swelling and related disorders. Adequate evidence is not available to support its medicinal properties for further use and drug development. Present study was designed to evaluate its traditional use in pain and inflammation with further characterisation of its chemical constituents through liquid chromatography-mass spectroscopic (LC-MS) analysis. Antinociceptive and analgesic potential of methanol extract of the aerial parts of C. diurnum was carried out using carrageenan induced paw oedema and formalin induced paw licking test in mice at the oral doses of 150 and 300 mg/kg body weight. Inhibition of the inflammatory mediator nuclear factor kappa B (NF-κB) was evaluated by tumour necrosis factor α (TNF-α) induced NF-κB activation assay in macrophage cells at the concentration of 100 μg/ml. LC-MS analysis of the extract was performed to characterise the active component responsible for bioactivities. The extract significantly inhibited (p < 0.05) carrageenan induced paw oedema at both doses tested and the effect persisted throughout the entire experimental period of 3 h with the highest activity (50% inhibition) observed at 3rd h. Further, the extract significantly inhibited (p < 0.05) formalin induced paw licking both in the early and late phase at the aforementioned dose levels. The extract also downregulated the expression of NF-κB p65 protein in the TNF-α induced NF-κB activation assay. LC-MS analysis of the extract indicated the presence of some important secondary metabolites including nicotine, nornicotine, ursolic acid, vitamin D3 and its derivatives. The results of this study supported the folkloric uses of the plant in pain and inflammations. The insights and observations suggest the action might involve downregulation of NF-κB p65 protein expression and/or inhibition of autacoids (histamine, serotonin, prostaglandin).


Introduction
Cestrum diurnum L., (Family: Solanaceae), the dayblooming jasmine, day jasmine, day-blooming cestrum, wild jasmine, Chinese inkberry, locally known as Buno-Hasnahena in Bengali is an erect evergreen woody shrub with numerous leafy branches and simple leaves [1][2][3][4][5][6][7]. The plant yields sweet smelling white flowers in short clusters and black fruits which are nearly globular shaped berries. The plant is originated from West Indies and eventually dispersed throughout the world and became naturalized throughout the tropical and subtropical regions of India and Bangladesh.
C. diurnum has been well documented in medicinal plant repositories and herbal compendia, and used as herbal raw material for the management of pain and inflammatory disorders in complementary and alternative medicine throughout the globe. The plant extract was found to be safe and well tolerated in several clinical trials [2,7,12]. Nevertheless, adequate evidence is not available to support its therapeutic value in the treatment of pain and inflammation with underlying mechanism. Subsequently, present study was designed to investigate the aerial part of C. diurnum for analgesic, anti-inflammatory activities, to provide scientific evidence for its use as a conventional folk medicine, determine its role on the inflammatory mediator nuclear factor kappa B (NF-κB) and characterise its secondary metabolites with medicinal importance.

Collection and identification of plant material
Aerial parts of the plant Cestrum diurnum L. (Family: Solanaceae) was collected from Dumuria area of Khulna district, Bangladesh (Coordinate 22.8083° N, 89.4250° E). The plant specimen was identified by the experts at Bangladesh National Herbarium (voucher specimen no. DACB 38792) [23].

Test animals
Young Swiss albino mice of either sex, 3-4 weeks old, weighing 20-25 g were purchased from the Animal Resources Branch of the International Centre for Diarrhoeal Diseases and Research, Bangladesh (ICDDR,B) [24]. The animals were kept in the animal house of Manarat International University under standard laboratory conditions (relative humidity 55-65%, room temperature 25.0 ± 2.0 °C and 12 h light-dark cycle) for adaptation [23]. They were fed with ICDDR,B formulated pelleted standard diet and water ad libitum.

Extract preparation
Shed dried plant material was ground into a coarse powder and stored in an airtight container until extraction commenced [24]. Powered plant material (135 g) was macerated in 400 ml of methanol (Merck, Germany) for 3 days with occasional sonication. The filtrate was evaporated to dryness using a rotary vacuum evaporator to get the crude extract (yield 7.4% of the dry powder).

Carrageenan-induced paw oedema test
Anti-inflammatory activity was tested by carrageenan induced paw oedema model in mice [25]. In brief, 0.1 ml of 1% w/v carrageenan suspended in 1% carboxymethylcellulose was injected into the sub-plantar tissue of the left hind paw of each mouse as the chemical inducer of chronic inflammation. Mice were divided into four groups containing six animals in each. Test groups received plant extract at the doses of 150 and 300 mg/kg body weight, while control and positive control group received vehicle and diclofenac sodium (10 mg/kg body weight), respectively. The paw thickness was measured using a calliper at 0, 60, 120 and 180 min after the carrageenan injection. Inhibition of oedema was calculated using the following formula where T t and T 0 are the thickness of paw oedema in test/positive control and control group, respectively.

Formalin induced paw licking test
A formalin-induced nociception model was employed to evaluate the central analgesic activity of the extract [24,26,27]. Mice were grouped in control, test and positive control receiving vehicle, extract (150 and 300 mg/ kg body weight, per oral) and diclofenac sodium (10 mg/ kg body weight, intraperitoneally), respectively. Formalin solution (0.2 ml of freshly prepared 5% v/v formalin in distilled water) was injected into the dorsal surface of the right hind paw 30 min after the treatments. The mice were observed for the time spent for apparent spontaneous pain behaviours, i.e., licking of the injected hind paw during the early phase (0-5 min) and late phase (15-30 min) of formalin injection. The biting, flinching, shaking, and favouring of the injected paw were also monitored [26,27].

TNF-α induced NF-κB activation assay
To elucidate the possible role of C. diurnum extract on the inflammatory mechanism, a NF-κB translocation activity was performed using NF-κB activation kit (Ther-moFisher Scientific, Waltham, MA) [28]. For this purpose, RAW 264.7 macrophage cells (Sigma-Aldrich) were seeded in 96-well plate overnight and then treated with (a) C. diurnum extract alone at 12.5 μM or (b) 10 ng/ml TNF-α alone or (c) 12.5 μM of C. diurnum extract and 10 ng/ml TNF-α in combination. Then 4% formaldehyde was used to fix the cells for 15 min followed by the addition of 0.1% Triton X-100 in phosphate buffer saline (PBS) to make the cells permeable. The cell were then incubated with NF-kB primary rabbit antibody to detect the endogenous levels of total NF-κB protein to determine the involvement of the NF-κB protein in the priming of autoimmune T cell responses [28,29]. The cells were then treated with goat anti-mouse secondary antibody (IgG) conjugated with staining solution DyLightTM 649 for 1 h. The cells were then rinsed in triplicate with wash buffer II (1 X PBS with 1% Tween-20). The nuclei were stained with Hoechst 33258 to visualized them. Finally, the plate with stained cells were imaged using Cellomics ArrayScan HCS reader (ThermoFisher Scientific, Waltham, MA). Data were captured, extracted and analysed with ArrayScan II Data Acquisition and Data Viewer version 3.0 (Cellomics).

Liquid chromatography-mass spectrometry (LC-MS) analysis
LC-MS analysis of the extract was performed to characterise phytochemical content with possible link to the obeserved bioactivities [24]. High-resolution electrospray ionisation (HR-ESI) mass spectra were recorded on 6530 Accurate-Mass Q-TOF LC/MS spectrometer (Agilent Technologies) equipped with ZORBAX Eclipse XDB-C18 Rapid Resolution column (HT 4.6 mm i.d. × 50 mm × 1.8 μm). The extract was injected at a volume of 10 μl (1 mg/ml) with a flow rate of 1 ml/min. A gradient elution with solvent A (1% trifluroacetic acid in water) and B (MeOH) starting at 95%A5%B to reach 100%B in 40 min was adopted. Other parameters include capillary voltage of 3500 V, nebuliser pressure 35 psi, drying gas flow 8 L/min, drying gas temperature 200 °C. The mass was recorded in the range of m/z 100-1000 and expressed as total ion chromatogram (TIC).

Data analysis
Values from animal model analyses are expressed as mean ± SEM of six replicates. Results were analysed using Microsoft Excel. Statistical analyses were done using one-way ANOVA followed by Dunnett's test. Statistical differences between control and test groups were calculated by Student's t-test and were considered as statistically significant at p < 0.05.

Results of carrageenan-induced paw oedema
The carrageenan-induced paw oedema used in this study is a well-accepted model to evaluate natural products against acute inflammation [30]. C. diurnum extract prevented carrageenan-induced paw oedema throughout the entire experimental period of 3 h at the doses of 150 and 300 mg/kg. The experiment also demonstrated that the result observed for test extract at the dose of 300 mg/ kg was comparable to that of diclofenac sodium, used as positive control in this study ( Table 1).

Results of formalin induced paw licking
C. diurnum extract, at the doses of 150 and 300 mg/kg, significantly (p < 0.05) suppressed the licking activity both in the early and late phase of formalin-induced pain in mice as compared to the control ( Table 2). The observed effect was stronger at the higher dose (300 mg/kg) of the extract tested.

Effect on TNF-α induced NF-κB activation
In the absence of TNF-α, NF-κB p65 remains in the cytoplasm of RAW 264.7 macrophage cells. In response to the stimulation by TNFα, NF-κB p65 protein translocates from cytoplasm into the nucleus implying NF-κB activation. C. diurnum extract did not inhibit TNF-α -mediated nuclear translocation of NF-κB p65 very strongly at 100 μg/ml although the result was significantly different from that of control (Fig. 1). Interestingly, the total NF-κB p65 protein expression was found downregulated, indicating the extract exerts its anti-inflammatory effect through the inhibition of NF-κB p65 protein expression (Fig. 2).

Results of LC-MS analysis
LC-MS analysis of C. diurnum revealed the presence of some pharmacologically important secondary metabolites already reported earlier from this species, which include vitamin D 3 and its derivatives (Table 3). Total ion chromatogram and MS spectrum for the major peaks is provided in the supplementary file.

Discussion
Inflammation and the search for its cure has been the centre of attention for last few decades due to its involvement to many diseases including arthritis, cancer,   diabetes, obesity and neurodegeneration. Although inflammatory response is a vital defensive mechanism of human body, chronic inflammation can lead to various pathological conditions such as chronic infection and autoimmune diseases [31,32]. Inflammatory process is a complex cascade of events that encompass cellular response with subsequent release of inflammatory molecules (e.g. COX-2, 5-LOX and TNF-α) regulated by pro-inflammatory transcription factors (e.g. NF-κB and STAT3), and activation of other cells (e.g. leucocytes and platelets) [33]. Inhibition of NF-κB is considered as one of the most important targets to control inflammation since this regulatory protein serves as the key checkpoint for inflammatory responses [34,35]. Some natural products including curcumin, emodin, capsaicin and resveratrol have been found to inhibit NF-κB. However, lack of bioavailability and other issued hindered their way to become therapeutic agents [36]. Natural products still serve as one of the major sources for new drug discovery for the treatment of pain, inflammation and related disorders. Plants used in the treatment of inflammatory disorders in ethnobotanical   [9] practices may hold the promise to be investigated for novel anti-inflammatory agents [3][4][5][6][7]. In this study, C. diurnum, a well-documented medicinal plant used in the traditional and complementary health care systems for the treatment of joint pain and swelling. Carrageenan induced paw oedema and formalin induced paw licking, two most widely accepted animal models to screen anti-inflammatory activity of crude extracts or pure compounds, were used for the investigation of analgesic and anti-inflammatory activity of the methanol extract of C. diurnum aerial parts [25,[30][31][32]. In addition, the extract was further tested in RAW 264.7 cells for its possible role on NF-κB protein [28]. Methanol was chosen for maceration of plant material due to its ability to extract a wide range of secondary metabolites of both polar and nonpolar nature with the added advantage of the ease of its removal.
In the carrageenan-induced acute inflammation test, the total duration of carrageenan-induced paw oedema in mice model is represented by a biphasic model. The first phase of inflammation is triggered by histamine and serotonin released after carrageenan injection and lasts for about two and a half hours [37]. The second phase of inflammation is due to the prostaglandin synthesis which starts after the first phase and can last up to 6 h [25,38]. In this study, both the test extract and diclofenac sodium produced mild inhibition of paw oedema in the early phase of inflammation, however the inhibition was more noticeable during the late phase indicating their key inhibitory role on cyclooxygenase mediated prostaglandin synthesis.
Again, the biphasic model of formalin-induced nociception can be categorized into neurogenic (0-5 min) and inflammatory pain (15-30 min), respectively. The early phase of neurogenic pain is due to the chemical stimulation of the nociceptors predominantly C-fibres, while the late phase of tonic pain is a combination of peripheral inflammatory responses and functional changes in spinal cord level [39]. Drugs that act primarily on the central nervous system such as narcotic analgesics inhibit both phases while steroids and NSAIDs suppress mainly the late phase [40]. In this study, the extract suppressed the gradual increase of paw licking in both phases which implies that it may contain constituents that act both centrally and peripherally. On top of this, formalin-induced nociception model is also recognized as one of the most suitable evaluation test procedures against chronic inflammation, as it closely resembles human arthritis [41]. Thus, both the approaches carried out in this study successfully support the use of this plant in ethnobotanical practice for alleviating pain and inflammatory conditions and explaining its utility in arthritic pain.
To understand the underlying mechanism of antiinflammatory effect, RAW 264.7 cells were treated with the extract before stimulating them with TNF-α, a potent inducer and activator of NF-κB. The NF-κB protein family is a central mediator of inflammatory responses and plays a crucial role in the development of acute or chronic inflammation [28,42]. It consists of five subfamilies that share same DNA binding domain and dimerisation domain. The NF-κB proteins are associated with each other to form homo-or heterodimeric complexes, which remain inactive in the cytoplasm by sequestering with inhibitory proteins (IκB). Upon stimulation by various factors including lipopolysaccharides (LPS) or TNF-α, IκB is degraded and dissociated from NF-κB. As a result, NF-κB migrates from the cytoplasm into the nucleus and triggers the transcription of numerous genes involved in pro-inflammatory responses [43,44].
Chemical inducer of pain like low doses of acetic acid, formalin and carrageenan causes the activation of peritoneal macrophages and mast cells, the cellular sites of cyclooxygenase enzyme activity during acute peritoneal inflammation, that leads to the local release of cytokines such as TNF-α and interleukin 1β as well as other pain mediators [45]. In this study, effect of the C. diurnum aerial extract was compared to the effect induced by chemical inducers and the extract was found to inhibit tumor necrosis factor α (TNF-α) induced NF-κB activation.
Various classes of natural products have been identified with the capability to downregulate NF-κB, prevent nuclear translocation of NF-κB and/or inhibit their DNA binding [46,47]. The results in this study indicate that C. diurnum extract can downregulate the expression of NF-κB p65 protein. Literatures on NF-κB inhibitory natural products reveal that most of the identified compounds prevent nuclear translocation of NF-κB while very few of them such as catechin and epigallocatechin gallate act through the downregulation of NF-κB expression [48,49]. Therefore, the extract may be an important source of NF-κB inhibitor that acts through its downregulation.
LC-MS analysis of the crude extract revealed the presence of a number of bioactive compounds including ursolic acid, nicotine and nornicotine. Ursolic acid, a ubiquitous plant pentacyclic triterpene, was found in a previous study to inhibit prostaglandin biosynthesis through the inhibition of COX-2 enzyme [50]. However, another study showed that the anti-inflammatory effect of ursolic acid involves to the inhibition of NF-κB activation and its DNA binding [51]. Thus, the downregulation of NF-κB by C. diurnum extract might be due to compound(s) other than ursolic acid. Nicotine and nornicotine cannot be ruled out as well since they were reported for anti-inflammatory [52] and analgesic [53] activity, respectively. To elucidate the molecular mechanisms of the bioactive components, the bioactivity of individual components could be analysed using in vivo cell-specific approaches or in silico assays [54][55][56]. Concurrent occurrence and bioavailability of two or more components may synergies or antagonise the medicinal activity of each other. As the extract possessed multiple components together, combined effect of the components could be observed for any agonism or antagonism [57].
Inflammation is a physiological response to injury, microbial attack or malignancy [58]. Pain is just one of the manifestations of the inflammatory responses and prolonged inflammation can aggravate an already existing disease as well as new disease progression including cancer [59]. Present study suggests that the plant C. diurnum can be useful in the treatment of pain, inflammation and diseases that involves NF-κB mediated inflammatory response.

Conclusion
This preliminary study with C. diurnum is a part of the continuing endeavour to identify medicinal plants that find their use in pain and inflammation in traditional medicine [60][61][62][63]. Results of the current investigation underpinned the traditional use of C. diurnum in inflammatory pain. In parallel, the insight of this study implies that the plant may not be suitable for long-term use due to the chance of over exposure to vitamin D 3 and its derivatives, however proved itself interesting enough for bioassay guided study to find new natural anti-inflammatory agent. Inhibition of prostaglandin synthesis and downregulation of NF-κB p65 protein synthesis could be the underlying mechanisms for the observed activity. This is the first report of antinociceptive, anti-inflammatory and NF-κB inhibitory activity of the plant C. diurnum which provides validation to currently unavailable evidences of its traditional uses as well as opens the door to develop new drug molecule against pain and related disorders.
Additional file 1: Figure S1. Total ion chromatogram (TIC) of C. diurnum extract. Figure S2. MS spectrum of C. diurnum extract at 24.72 min. Figure  S3. MS spectrum of C. diurnum extract at 32.26 min. Figure S4. MS spectrum of C. diurnum extract at 24.97 min. Figure S5. MS spectrum of C. diurnum extract at 21.58 min. Figure S6. MS spectrum of C. diurnum extract at 23.28 min. Figure S7. MS spectrum of C. diurnum extract at 29.44 min. Figure S8. MS spectrum of C. diurnum extract at 21.58 min. Figure S9. MS spectrum of C. diurnum extract at 32.69 min.