Liver mitochondrial membrane permeability modulation in insulin-resistant, uninephrectomised male rats by Clerodendrum volubile P. Beauv and Manihot esculenta Crantz

Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Table 1 Percentage induction of mitochondrial membrane pore opening in rat liver mitochondria incubated with methanolic extract of Clerodendrum volubile leaf and ethanolic extract of Manihot esculenta leaf, in vitro

% Induction	NPD-C		NPD-UNX		HFD-Sham		HFD		UNX-HFD		HFD-UNX
% Induction	Cv	Me	Cv	Me	Cv	Me	Cv	Me	Cv	Me	Cv	Me
NTA	13.98 ± 6.53	13.98 ± 6.53	23.49 ± 8.30	23.49 ± 8.30	26.38 ± 7.30	26.38 ± 7.30	23.59 ± 15.58	23.59 ± 15.58	3.10 ± 0.80	3.10 ± 0.80	50.55 ± 5.87	50.55 ± 5.87
TA	^a86.02 ± 10.75	^a86.02 ± 10.75	^a76.51 ± 19.85	^a76.51 ± 19.85	a73.62 ± 10.05	^a73.62 ± 10.05	^a76.41 ± 15.71	^a76.41 ± 15.71	^a96.90 ± 14.36	a96.90 ± 14.36	49.45 ± 13.73	49.45 ± 13.73
200 μg/ml	^b30.19 ± 6.15	^b16.36 ± 9.66	^b96.64 ± 10.60	^b158.24 ± 14.71	^b84.64 ± 10.22	^b44.34 ± 11.73	^b195.23 ± 24.72	^b207.58 ± 27.08	^b44.34 ± 15.91	^b41.82 ± 14.54	^b65.56 ± 10.00	^b53.00 ± 10.17
600 μg/ml	^b37.38 ± 16.06	^b9.10 ± 1.94	^b38.43 ± 14.30	^b70.31 ± 12.09	^b217.93 ± 22.51	^b11.87 ± 1.77	^b225.84 ± 24.74	^b63.57 ± 9.79	^b11.87 ± 7.75	^b80.47 ± 10.07	^b46.69 ± 9.13	^b38.54 ± 7.24
1000 μg/ml	^b45.68 ± 16.45	^b8.74 ± 2.97	^b23.49 ± 9.99	^b78.73 ± 16.80	^b175.79 ± 22.91	^b32.57 ± 16.97	^b221.52 ± 28.46	^b75.18 ± 11.45	^b32.57 ± 14.05	^b67.16 ± 17.82	^b142.46 ± 17.47	^b185.85 ± 23.59
1400 μg/ml	^b37.26 ± 16.13	^b53.08 ± 18.96	^b14.19 ± 6.15	^b143.88 ± 13.63	^b47.27 ± 14.42	^b17.13 ± 8.06	^b131.38 ± 18.61	^b148.04 ± 17.21	^b17.13 ± 3.52	^b133.85 ± 17.29	^b120.22 ± 15.54	^b129.78 ± 16.94

Cv Clerodendrum volubile P. Beauv, Me Manihot esculenta Crantz (red stalk), NPD-C Normal Pellet Diet-fed, Control, non-nephrectomized, NPD-UNX Normal Pellet Diet-fed, Uni-nephrectomized, HFD-Sham High Fat Diet-fed, surgically opened, non-nephrectomized, HFD High Fat Diet-fed, uni-nephrectomized, UNX-HFD Uni-nephrectomized before High Fat Diet-fed, HFD-UNX High Fat Diet-fed before uninephrectomized, NTA No triggering agent, negative control (no treatment with exogenous calcium, only energized with sodium succinate), TA Triggering agent, positive control (treatment with exogenous calcium to represent calcium overload which triggers pore opening). ^asignificantly different when compared with NTA effect across all groups at p < 0.05. ^bSignificantly different from TA effect across all groups at p< 0.05