International Journal of Phytomedicine and Phytotherapy
Table 1 One Step RT-qPCR Reaction Conditions
Stages | Cycles | Program in Standard / Fast Mode |
|---|---|---|
1. Reverse Transcription | 1 | ≥37 °C for 15 min |
2. RT inactivation/Hot-start activation | 1 | 95 °C for 10 min |
3. 3-Step qPCR | ||
i. Denature | 40 | 95 °C for 10 s |
ii. Anneal/ Collect Data | 56 °C for 30 s | |
iii. Extend | 72 °C for 30 s | |
4. Dissociation | 1 | 95 °C |