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International Journal of Phytomedicine and Phytotherapy

Table 1 One Step RT-qPCR Reaction Conditions

From: Cardioprotective effect of garlic extract in isoproterenol-induced myocardial infarction in a rat model: assessment of pro-apoptotic caspase-3 gene expression

Stages Cycles Program in Standard / Fast Mode
1. Reverse Transcription 1 ≥37 °C for 15 min
2. RT inactivation/Hot-start activation 1 95 °C for 10 min
3. 3-Step qPCR
 i. Denature 40 95 °C for 10 s
 ii. Anneal/ Collect Data 56 °C for 30 s
 iii. Extend 72 °C for 30 s
4. Dissociation 1 95 °C