Fig. 4
Photomicrograph of the liver Fig. a-d and kidney Fig. e-g of albino rat under different experimental conditions. Fig. a-d show the histomorphological manifestation of the hepatocytes, density of hepatocytes, distribution of hepatocytes, staining intensity of hepatocytes, size of central veins, content of central veins and expression of large vacuolations (dotted black circles). Large vacuolations with pick coloration are fatty livers with bile plaques suggesting cholestasis. Kidney slices Fig. e-g of experimental animals showing a high-power magnification (x400mag) of renal corpuscle (black outline). The histomorphology presents with the convoluted tubule (CT), glomerular capillaries (GC) and inherent cells which include the intraglomerular podocytes (P) as well as the juxtaglomerular cells and macula densa cells in the vascular poles of the renal corpuscles. The urinary pole continues out as the proximal convoluted tubules. a- liver slice of animals fed with animal feed and distilled water only; Normal histomorphology of liver tissue presenting with typical cellular density and cellular distribution. The nuclei of hepatocytes are distinctively stained and properly disposed within their respective cytoplasm. There are no histopathological alterations in the histological presentation of these tissues. b- liver slice of animals administered with 3 ml/kg CCl4, Fatty liver with cholestasis. Typical cellular density and cellular distribution. The nuclei of hepatocytes are distinctively stained and properly disposed within their respective cytoplasm. c- liver slice of animals induced with 3 ml/kg CCl4 and treated with 100 mg/kg P. americana; Normal histomorphology of liver tissue presenting with typical cellular density and cellular distribution. The Nuclei of hepatocytes are distinctively stained and properly disposed within their respective cytoplasm. There are no histopathological alterations in the histological presentation of these tissues. d - liver slice of animals administered with 3 ml/kg CCl4 and treated with 100 mg/kg Silymarin; Normal histomorphology of liver tissue presenting with typical cellular density and cellular distribution. The Nuclei of hepatocytes are distinctively stained and properly disposed within their respective cytoplasm. There are no histopathological alterations in the histological presentation of these tissues. e - kidney slice of animals administered with distilled water only; normal renal corpuscle with typical cellular delineation, distribution, density and staining intensity. No apparent histopathological alteration. f - kidney slice of animals administered with 250 mg/kg rifampicin only; Distortion of the renal architecture and atrophy of glomeruli was observed. The renal tubules were degenerated and showed intraluminal exfoliation with granular cast formation as well as pyknosis of the nuclei. g- kidney slice of animals administered with 250 mg/kg rifampicin and treated with 100 mg/kg bw of P. americana