Phytochemicals and in vitro anti-apoptotic properties of ethanol and hot water extracts of Cassava (Manihot esculenta Crantz) peel biogas slurry following anaerobic degradation

Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

Table 1 Procedure for the assay of mitochondrial membrane transition pore in rat liver mitochondria (MMPT)

Sample	MSH\(\text{b}\text{u}\text{f}\text{f}\text{e}\text{r}\) (\({\upmu }\text{l})\)	Rotenone \(\left({\upmu }\text{l}\right)\)	Spermine \(\left({\upmu }\text{l}\right)\)	Extract \(({\upmu }\text{l}\))	MIT \(({\upmu }\text{l}\))	Calcium \(\left({\upmu }\text{l}\right)\)	Succinate \(({\upmu }\text{l}\))
NTA	2385	25	---	---	30	---	50
TA	2360	25	---	---	30	25	50
I	2297.5	25	62.5	---	30	25	50
E10 (µg/ml)	2330	25	---	10	30	25	50
E30 (µg/ml)	2330	25	---	30	30	25	50
E50 (µg/ml)	2310	25	---	50	30	25	50

NTA No triggering agent, TA Triggering agent, I Inhibitor, E Extract at different concentrations, MSH Buffer Swelling buffer, MIT Isolated mitochondria
Absorbance was read at 540 nm at 30-sec intervals over 12 minutes, and the assay was conducted in duplicates (24 readings, n =3).
Δ Abs = Abs at T_{(30 s, 1 min, 1 min 30 s …12 min)} – Abs at T_{0 sec}.