Skip to main content

International Journal of Phytomedicine and Phytotherapy

Clinical Phytoscience Cover Image

Sorghum halepense (L.) Pers rhizomes inhibitory potential against diabetes and free radicals



Owing to the side effects of synthetic medicine and less effectiveness against different syndromes, the researchers have focused on phytotherapy to overcome these problems. The purpose of this project was to study the in vitro phytochemical, cytotoxic, total phenolic, antioxidant and antidiabetic activities of the methanol extract of the rhizome of Sorghum halepense (L.) Pers and its n-hexane, chloroform and aqueous fractions. Thereafter, to conduct in vivo evaluation of the effective extract for its antidiabetic and antioxidant characteristics.


Cytotoxic, total phenolic content and antidiabetic properties were ascertained by brine shrimps lethality, Folin- Ciocalteu reagent and alpha-amylase inhibition assays respectively while antioxidant activities were investigated through DPPH, ABTS and H2O2 assays. The methanolic extract was assessed in vivo for its antidiabetic and antioxidant activities by using Wistar albino rats.


The phytochemical investigation of the methanolic extract and its unlike fractions revealed the availability of alkaloids, cardiac glycosides, flavonoids, terpenes, steroids, carbohydrate and proteins while lack of saponins and gums in methanolic extract. Steroids and carbohydrates were only present in aqueous and chloroform fraction respectively while both fractions contained proteins and alkaloids. Cardiac glycosides and flavonoids were absent in aqueous and chloroform fractions respectively. The highest brine shrimps lethality (70.5 ± 1.2), total phenolic content (28.30 ± 1.3 mg GAE/g), free radicals scavenging potential i.e. DPPH (40.02%), ABTS (40.48%) and H2O2 (50.85%) and alpha amylase inhibition (61.87%) was shown by the methanolic extract. The in vivo results did not disclose any sign of acute toxicity. The diabetic control showed a noteworthy (P < 0.05) decline in weight, HDL and glutathione and a raised level of bilirubin, blood glucose, urea, creatinine, triglyceride, LDL, VLDL, ALT, ALP, AST, SOD, catalase. The mentioned alterations were restored considerably (P < 0.05) by treatment of diabetic rats with methanolic extract of Sorghum halepense (L.) Pers (150 and 300 mg/kg b.w.).


It is concluded that the extract of rhizomes of Sorghum halepense (L.) Pers is an effective fount of antioxidant and anti-diabetic compounds. Further analysis is needed to sharpen its pharmacological activities.


The Sorghum genus, belonging to the Poaceae, constitute about 44% of weed species that emerge, overrun and cause severe damages to agriculture and livestock in the world [1]. Sorghum halepense (L.) Pers, one of its species is known as the most popular weed in the world as it has elevated infestation potential on pastures and crops. Both seeds and rhizomes can grow to reproduce this perennial rhizomatous weed of summer [2]. The habitats of Sorghum halepense (L.) Pers includes fields, open forests, wetlands and ditches [3].

It is a rich source of several significant compounds including oxazolidinone, taxiphyllin, chlorogenic acid, sorgoleone prunasin, and dihydrosorgoleone p-coumaric acid, hydroxyl benzyl alcohol, p-hydroxybenzoic acid, p-hydroxybenzaldehyde, p- phloroglucinol, aliphatic acids from the rhizomes of S. halepense (L.) Pers [4,5,6].

Plants possess numerous therapeutically active chemical constituents. Most of these compounds produced by the plants are extracted in various solvents and their curative effects are identified.

A chronic metabolic disorder, diabetes mellitus, is characterized by improper regulation of carbohydrate, protein, and lipid metabolism due to malfunction or deficiency in insulin production [7]. It is also associated with hyperglycemia, which in turn, through the non-enzymatic glycation and self-oxidation of glucose [8] leads to oxidative stress [9]. This oxidative stress has been observed to be closely associated with complications of diabetes mellitus, including nephropathy, anemia, hepatopathy [9] dyslipidemia, the primary threat for cardiovascular ailment [10].

Materials and methods

Chemicals and reagents

Analytical grade chemicals including sodium phosphate dibasic, sodium phosphate monobasic, ABTS, DPPH, hydrogen peroxide, n-hexane, chloroform, Folin-Ciocalteu reagent, Na2CO3, sea salts, gallic acid, SDS, TBA, NaCl, KCl, alloxan, normal saline, ascorbic acid, starch, glucophage, amylase, DNS (dinitrosalicylic) acid, potassium persulfate, Benedict’s solution, Ferric chloride, Molish’s reagent, sulphuric acid, Libermann- Burchard reagent, Mayer’s reagent, were used during biological activities. All the mentioned chemicals were purchased from Sigma-Aldrich except glucophage which was purchased from Merck.

Plant materials

In March 2017, the plant of Sorghum halepense (L.) Pers was collected from District Bannu, Pakistan. It was recognized by Prof. Abdur Rehman, Government Post Graduate College Bannu, KPK, Pakistan and deposited a specimen voucher (AR-125) in the herbarium of University of Science and Technology Bannu, KPK, Pakistan. Thereafter their rhizomes were collected and shade dried.

Preparation of extract

The rhizomes of juvenile Sorghum halepense (L.) Pers were collected, washed thoroughly, shade dried and pulverized into small pieces by using pestle and mortar. A 70% methanol solution (2 L) was used to extract the active compounds from the pulverized rhizomes (800 g) by submerging it in the mentioned solution with regular shakeup for 72 h at room temperature. It was filtered (Whatman No. 3 filter paper, Whatman Ltd., England), the filtrate was dried under rotary vacuum evaporator (Strike202, Italy) at 40 °C to obtained concentrated sticky methanolic extract (23.56 g) and stored for the future use.

Fraction’s preparation

20 g of methanolic extract was sequentially fractionated with n-hexane, chloroform and aqueous (400 ml each) by using separating funnel. Filtrates of n-hexane (1.87 g), chloroform (4.93 g) and aqueous (7.973 g) fractions were evaporated at room temperature and stockpiled the gummy portion for further analysis.

Phytochemical screening

Methanolic extract of Sorghum halepense (L.) Pers rhizomes and its various fractions were phytochemically evaluated to verify the existence or lack of terpenes, cardiac glycosides, flavonoids, steroids, carbohydrate, proteins, saponins and alkaloids according to standard methods [11].

Brine shrimp lethality assay

Methanolic extract and its different fractions of rhizomes of young Sorghum halepense (L.) Pers were introduced into the biological analysis of shrimp (Artemia salina) mortality to determine their cytotoxic properties [12]. Artificially prepared 4% seawater was poured into a container comprising on two chambers, one covered with aluminum foil and another illuminated with energy saver lamp, suspended I mg brine shrimp egg in a covered chamber for 24 h. The shrimps after hatching crossed the central porous wall and came to the luminous chamber. Different concentrations i.e. 100, 250, 500 and 1000 μg/ ml of each sample were prepared in methanol. The samples (1 ml) were put into the experimental test tubes containing artificial seawater and allowed complete evaporation of methanol from it. The control lacked the samples. Ten shrimps were added to all test tubes, incubated for 24 h and thereafter in each test tube, the live shrimps were counted. The % lethality of shrimps was calculated by Abbot’s formula.

$$ \%\mathrm{Death}\kern0.5em =\kern0.5em \left(\mathrm{Sample}-\mathrm{control}/\mathrm{control}\right)\kern0.5em \times \kern0.5em 100 $$

Total phenolic content

Folin-Ciocalteu reagent [13] was applied for the determination of total phenolic content in the rhizomes of Sorghum halepense (L.) Pers methanolic extract and its fractions. The working solution of Folin–Ciocalteu reagent was prepared by its 10X dilution in distilled water whereas 1–5 mg/ml sample solutions were prepared in respective solvents. Sample solution (250 μl) was mixed with a working solution of Folin–Ciocalteu reagents (2.5 ml) and incubated for 5 min at room temperature. 2.5 ml saturated solution (60 mg/ml) of Na2CO3 was poured into the reaction mixture and incubated once more at the said temperature for 2 h. Instead of a sample solution, gallic acid was added to standard/ control. The absorbance was measured at 725 nm spectrophotometrically and the findings were shown as gallic acid equivalent.

Antioxidant assays

DPPH assay

A commonly adopted antioxidant assay, DPPH assay was followed according to the standard [14] procedure to find out the antioxidant potential of rhizomes of Sorghum halepense (L.) Pers. Different working solutions (125, 250, 500, 1000, 1500 and 2000 μg/ml) of all samples and ascorbic acid were prepared. DPPH solution (3 mg in 100 ml) was prepared, incubated at 25 °C in a baker fully covered with aluminum foil for half an hour; thereafter its absorbance was measured at 517 nm and adjusted it to less than one. The sample /ascorbic acid solutions were poured to DPPH solution and measured the absorbance spectrophotometrically. Their free radical scavenging capabilities were determined as follows.

$$ \mathrm{Percent}\ \mathrm{scavenging}\ \mathrm{of}\ \mathrm{DPPH}\ \mathrm{free}\ \mathrm{radicals}\kern0.5em =\kern0.5em \left({\mathrm{A}}_1-{\mathrm{A}}_2/{\mathrm{A}}_1\right)\kern0.5em \times \kern0.5em 100 $$

Where A1 = the control absorbance (DPPH only) and A2 = the experimental absorbance (DPPH + sample).

ABTS assay

The capability of prepared samples to scavenge ABTS free radical was assessed by using ABTS assay according to the standard procedure [15]. Potassium persulfate solution (2.45 mM) was added to ABTS solution (7 mM) and incubated overnight in the dark and thereafter relevant solvent (1:1) was added to it to manage its absorbance to 0.900 (±0.02) at 745 nm. The sample solution (300 μl: 125–2000 μg/ml in related solvent) was poured into the working solution of ABTS, incubated for 6 min and measured the absorbance. Ascorbic acid was used as a substitute for the sample in a control. The percentage competence of samples/ ascorbic acid to forage ABTS free radicals was computed as follows;

$$ \mathrm{Percent}\ \mathrm{scavenging}\kern0.5em =\kern0.5em \left[\left(\mathrm{control}\ \mathrm{absorbance}\ \left(\mathrm{ABTS}\right)-\mathrm{sample}\ \mathrm{absorbance}\right)\kern0.5em /\kern0.5em \left(\mathrm{control}\ \mathrm{absorbance}\right)\right]\kern0.5em \times \kern0.5em 100 $$

H2O2 assay

The H2O2 scavenging potential of prepared samples was investigated by following the procedure of Wettasinghe and Shahidi; 2000 [16]. 43 mM H2O2 solution was prepared in 100 mM phosphate buffer (pH 7.4) while the sample solutions (125–2000 μg/ml) were prepared in relevant solvents. The sample solution was poured into the reaction mixture, incubated for 40 min at room temperature and then measured its absorbance at 230 nm. H2O was added as a substitute for H2O2 in the blank. The percentage effectiveness of samples to scavenge H2O2 was computed as follows.

$$ \mathrm{Percent}\ \mathrm{scavenging}\ \mathrm{effect}\kern0.5em =\kern0.5em \left[\left(\mathrm{control}\ \mathrm{absorbance}\ \left({\mathrm{H}}_2{\mathrm{O}}_2\right)-\mathrm{sample}\ \mathrm{absorbance}\right)/\left(\mathrm{control}\ \mathrm{absorbance}\right)\right]\kern0.5em \times \kern0.5em 100 $$

All tests were carried out three times and articulated the findings as means ± SD.

Alpha-amylase inhibition

The capacity of samples to inhibit alpha-amylase was explored according to Worthington Enzyme Manual [17] guideline. Sodium phosphate buffer (pH 6.9, 20 mM with 6 mM NaCl) was used to prepare the dinitrosalicylic (DNS) acid, alpha-amylase and starch solutions. The sample (300 μL), 500 μL starch solution (1%) and 500 μL alpha-amylase solutions (0.5 mg/mL) were put together and kept for 10 min at 25 °C. DNS acid (1.0 mL) was poured into the reaction mixture to stop the enzymatic reaction, incubated in the boiling water for 5 min and subsequently cooled to room temperature. Distilled water (3 ml) was added to all test tubes to dilute the reaction mixture. The absorbance was measured spectrophotometrically at 540 nm. The percentage inhibition of alpha-amylase caused by the samples was calculated as follows.

$$ \mathrm{Amylase}\ \mathrm{inhibition}\ \left(\%\right)\kern0.5em =\kern0.5em \left[\left(\mathrm{control}\ \mathrm{absorbance}\left(\mathrm{Blank}\right)-\mathrm{sample}\ \mathrm{absorbance}\right)/\left(\mathrm{control}\ \mathrm{absorbance}\right)\right]\times 100 $$

In vivo study

Experimental animals

The experimental animals, male Wistar Albino rats (260–280 g b.w.) for the current study were bought from animal house, National Institute of Health Sciences (NIH) Islamabad. These animals were kept in cages under a standard husbandry environment (25 ± 0.5 °C; 12 h light/dark cycle), in reference to the guidelines approved by Institutional Animals Ethics Committee (IAEC number 123) and served with locally (Bannu) purchased pellet diet and fresh tap water. Prior to begin an experiment, the animals were acclimatized to the laboratory conditions in Bannu for 1 week.

Toxicity study

Two testings and one standard group, each with 6 healthy male Wistar Albino rats were fasted during the night and gave free access to the water. Different doses (1 and 2 g/kg b.w.) of methanolic extract of rhizomes of Sorghum halepense (L.) Pers were orally administered once (in 1 mL) to experimental groups to assess its possible toxicity. Mortality of the experimental animals as well as modification in their behaviors were examined for 24 h [18] and did not observe mortality or any other symptom of acute toxicity which indicated that LD50 of the tested extract is > 2000 mg/kg. Based on the result of an acute toxicity test, different doses (150 and 300 mg/kg) of extract were selected for the assessment of its antidiabetic characteristics.

Induction of experimental diabetes in rats

After fasting for 16 h, a single intra-peritoneal dose of freshly prepared alloxan (120 mg/kg b.w.) in a volume of 1 mL kg− 1 in normal saline was injected into each experimental rat so as to induce experimental diabetes in them [19,20,21,22]. The fasting blood glucose levels of rates were checked after 72 h [23] and rats with fasting blood glucose concentration ≥ 200 mg/dL were considered diabetic (in rats common range of blood glucose is 80–120 mg/dl) [21, 23, 24] and selected for designed experiments.

Experimental procedure

Following the confirmation of induction of diabetes in rats, they were grouped randomly into 5 groups of 5 rats each. All the rats within a group were numbered/ marked at their tails with the help of a permanent marker. Group 1: Normal rats (control); Group 2: Untreated diabetic rats (negative control); Group 3: Diabetic rats treated with glibenclamide at 10 mg/kg b.w; Group 4: Diabetic rats treated with methanolic extract of rhizomes of Sorghum halepense (L.) Pers at 150 mg/kg b.w; Group 5: Diabetic rats treated with methanolic extract at 300 mg/kg b.w. Plant extract and glibenclamide were fed orally to the rats for 21 days every morning with help of 16 gauge gastric intubation as the people conventionally exercise ethno medicines orally for the treatment of various ailments [25].

Quantification of body weight and blood glucose level

The fasting blood glucose concentrations were examined at the start prior to begin dose supply (t = 0; 1st day) and on 7th, 14th and 21st day of treatment with help of glucometer (Medisign, England). Blood samples from rats were obtained by aseptic puncture of their tail veins. The animals were weighed and articulated their initial (t = 1st day) and final (t = 21st day) weights [26].

Sacrificing animals and serum collection

Following the treatment with extract for 21st day, the rats were fasted overnight and anaesthetized on 22nd day. During anesthesia, each rat was placed in transparent pot which contained small cotton pad soaked with 4 ml diethyl ether (anesthetic ether) in which the rat became anaesthetized and insensible owing to inhalation of mentioned anesthetic drug and subsequently sacrificed. For obtaining serum, the collected blood by puncturing heart was kept in plain sample bottles, allowed to clot for 2 h and thereafter centrifuged for 10 min at 3000 rpm. The serum (supernatant) was collected for advanced investigations.

Lipid profile determination of serum

The level of triglycerides (TG), HDL and total cholesterol in blood serum was estimated with the help of chemistry analyzer (Selectra, XL, Netherlands) using commercially accessible kits (Gesan productions, Italy) in accordance with the manufacturer’s protocols. The level of very low density lipoproteins (VLDL) and low density lipoproteins (LDL) was computed by using Friedwald equation.

Assessment of liver and kidneys function

Aspartate and alanine aminotransferase activities (AST and ALT), creatinine and total bilirubin were found with help of chemistry analyzer (Selectra, XL, Netherlands) using commercially existing kits (Gesan productions, Italy) according to the manufacturer’s protocols.

Determination of malondialdehyde (MDA)

According to the published protocol, TBARS assay was conducted for the analysis of lipid per oxidation [27, 28]. Ice cold KCl solution (1.5%) was used to prepare 10% homogenate of liver tissues, centrifuged for 10 min at 4000 x g and collected the supernatant. 100 μL of supernatant was put without extract (control) or with extract (experimental) to the test tubes and kept at 37 °C for 60 min. Afterward incubation, 8.1% SDS, acetate buffer and TBA solution were put together and incubated once more for 60 min at 100 °C. The development of light pink color revealed the reaction of TBA with MDA. The absorbance was measured spectrophotometrically at 532 nm after ice cooling the tubes.

Estimation of glutathione level

Moron et al. (1979) protocol of spectrophotometric assay was opted to calculate glutathione (GSH) concentration in tissue homogenate [29]. The reaction of glutathione (GSH) (acid soluble sulphydryl groups) with DTNB (5, 50-dithiobis-2-nitrobenzoic acid) resulted in yellow color complex and showed the existence of glutathione [29]. During this course, tissue homogenate and 25% TCA were put together in proportion of 5:1 to precipitate the tissue homogenate and followed by centrifugation for 10 min at 3000 rpm. Subsequently, the 100 μL (supernatant) was mixed with the reaction mixture containing on 2 mL (0.6 mM) DTNB and 0.9 mL of sodium phosphate buffer (0.2 mM, pH 7.4) whereas the blank lack the supernatant. The development of yellow color complexes were measured against the blank at 412 nm. The molar extinction coefficient of DTNB (13,100/Mcm) was used for the estimation of glutathione level.

Determination of superoxide dismutase (SOD)

Beauchamp and Fridovich (1971) method was used to evaluate the activity of superoxide dismutase [30]. During the current experiment, the reaction mixture was comprised on 50 mM sodium carbonate (1 mL), 0.1 mM EDTA (200 μL), tissue homogenate (500 μL) and 400 μL of 25 μM NBT (nitro blue tetrazolium). The reaction was initiated by mixing 1 mM (400 μL) hydroxylamine–hydrochloride with the reaction mixture and measured the change in absorbance at 560 nm spectrophotometrically. The activity of SOD was measured as its quantity in units / ml that restrains 50% NBT reduction. The results of test (receiving extract) and normal groups were compared. The improved action of SOD shows an antioxidant characteristic.

Catalase activity

The serum catalase activity was determined by following the reported procedure of Atawodi [31]. 10 μL serum was mixed with 2.80 mL potassium phosphate buffer (50 mM, pH 7.0) followed by addition of 0.1 mL of fresh 30 mM H2O2 to commence the reaction. The breakdown rate of H2O2 was measured spectrophotometrically for 5 min at 240 nm and calculated the activity of catalase by applying the molar extinction coefficient of 0.041 mM / cm where its amplified activity indicates an antioxidant effect.

Statistical analysis

GraphPad prism software was used to analyze the results. All in vitro experiments were performed in triplicate while quintuple in vivo and showed the results as mean ± standard deviation. Furthermore, Pearson correlation coefficient was calculated between total phenolic versus antioxidant and antidiabetic activities. One way ANOVA and Dunnett’s-test were used for in vivo analysis. The p < 0.05 was considered statistically significant.


Phytochemical assessment

The phytochemical assessment of methanolic extract of rhizomes of Sorghum halepense (L.) Pers and its different fractions uncovered the presence of cardiac glycosides, flavonoids, terpenes, steroids, carbohydrates, proteins and alkaloids in methanolic extract. Carbohydrates and steroids were only present in aqueous and chloroform fractions respectively while both fractions contained proteins and alkaloids. Cardiac glycosides were absent in the aqueous fraction while flavonoids in the chloroform fraction (Table 1).

Table 1 Phytochemical evaluation of rhizomes of Sorghum halepense (L.) Pers methanolic extract and its aqueous, chloroform and n-hexane fractions

Brine shrimp lethality assay

The brine shrimps lethality bioassay revealed the cytotoxic characteristics of methanolic extracts of rhizome Sorghum halepense (L.) Pers and its different fractions. 70.5 ± 1.2, 50.4 ± 1.1, 50.3 ± 1.1 and 40.3 ± 1.6% lethality of brine shrimp against methanolic extract and its aqueous, n-hexane and chloroform fractions at the level of 1000 μg/ml was found respectively (Table 2).

Table 2 Percentage fatality of brine shrimps expressed by methanolic extract and its different fraction of rhizome of Sorghum halepense (L.) Pers

Total phenolic contents

The rhizome’s extract of Sorghum halepense (L.) Pers was explored for total phenolic content by using Folin Ciocalteu phenol reagent and gallic acid (standard). The contents of phenolic compounds in therapeutic plants are vastly capricious i.e. 2.34–152.32 mg GAE/g [32]. In the present project, the highest amount of phenolic contents was present in methanolic extract (28.30 ± 1.3) while lowest in n-hexane fraction (8.87 ± 1.35 mg GAE/g). Table 3 shows the results.

Table 3 Total phenolic content (mg GAE/g) of methanolic extract and its different fractions

Antioxidant assays

Plants are the major sources of natural antioxidants. The said antioxidants scavenge free radicals and their reactive derivatives (ROS), which are accountable for different disorders in human beings [33]. The mentioned ability of medicinal compounds was estimated by using the commonly used standard assays.

DPPH method

The estimation of primary antioxidant characteristics of plant extracts was found by opting DPPH assay where free radicals of DPPH are reduced. The antioxidant activities of methanolic extract of rhizomes of Sorghum halepense (L.) Pers, its prepared fractions and ascorbic acid (standard) were measured spectrophotometrically at 517 nm and compared [34]. Antioxidant capabilities of standard and samples are shown in Fig. 1.

Fig. 1

DPPH free radical scavenging capability of the rhizome of Sorghum halepense (L.) Pers methanolic extract and its fractions. Aqf: aqueous fraction, Cf: chloroform fraction, Hf: n-hexane fraction and Me: methanolic extract Asa: ascorbic acid. The scavenging effect of extract and ascorbic acid was compared. c indicates significance at p < 0.05, b at p < 0.01 and a at p < 0.001 (Dunnett’s-test). Performed the experiments in triplicate (n = 3)

The highest antioxidant activity was shown by methanolic extract (40.02%) followed by chloroform fraction (33.11%), aqueous fraction (32.86%), n-hexane fraction (23.79%) at the concentrations of 2 mg/ml. The ascorbic acid (standard) indicated 53.43% antioxidant activity at the same concentration. The antioxidant properties of methanolic extract of rhizome of Sorghum halepense (L.) Pers and its fractions were found concentration dependant.

ABTS radical cation assay

It is an easy and suitable assay for both hydrophilic and lipophilic antioxidants. During this assay, ABTS and potassium persulfate react together and a blue chromophore (ABTS•+) is generated. Plant extract or ascorbic acid (standard antioxidant) reduces the ABTS• + cation radical [35]. During the current study, methanolic extract (40.48%) exhibited the highest antioxidant activity followed by chloroform fraction (35.41%), an aqueous fraction (33.18%) and n-hexane fraction (23.81%) at the level of 2 mg/ml. The mentioned prospective of ascorbic acid (57.28%) was also articulated (Fig. 2).

Fig. 2

ABTS free radical scavenging capability of the rhizome of Sorghum halepense (L.) Pers methanolic extract and its fractions. Aqf: aqueous fraction, Cf: chloroform fraction, Hf: n-hexane fraction and Me: methanolic extract Asa: ascorbic acid. The scavenging effect of extract and ascorbic acid were compared. c indicates significance at p < 0.05, b at p < 0.01 and a at p < 0.001 (Dunnett’s-test). Performed the experiments in triplicate (n = 3)

Hydrogen peroxide (H2O2) scavenging capacity

The prepared plant extract of rhizome of Sorghum halepense (L.) Pers indicated scavenging of H2O2 in a concentration-dependent manner. The methanolic extract (50.85%), aqueous fraction (42.90%), n-hexane fraction (17.58%) and ascorbic acid (standard) expressed (52.22%) scavenging activity respectively at the concentration of 2 mg/ml. The chloroform fraction did not exhibit H2O2 scavenging potential at the mentioned concentration (Fig. 3).

Fig. 3

H2O2 free radical scavenging capability of the rhizome of Sorghum halepense (L.) Pers methanolic extract and its fractions. Aqf: aqueous fraction, Cf: chloroform fraction, Hf: n-hexane fraction and Me: methanolic extract Asa: ascorbic acid. The scavenging effect of extract and ascorbic acid were compared. c indicates significance at p < 0.05 (Dunnett’s-test). Performed the experiments in triplicate (n = 3)

Alpha-amylase inhibition

The results of alpha-amylase inhibition by methanolic extract of Sorghum halepense (L.) Pers rhizome and its different fractions were indicated in Fig. 4. The mentioned properties of available commercial medicine, glucophage (standard), the methanolic extract and chloroform fraction were measured 63.14%, 61.87%) and 22.66% respectively.

Fig. 4

Antidiabetic potency of methanolic extract of rhizome of Sorghum halepense (L.) Pers and its fractions, Glp: glucophage, Aqf: aqueous fraction, Cf: chloroform fraction, Hf: n-hexane fraction and Me: methanolic extract Asa: ascorbic acid. The scavenging effect of extract and ascorbic acid were compared. a indicates significance at p < 0.001 (Dunnett’s-test). (*Aqf: aqueous fraction and Hf: n-hexane fractions lacked antidiabetic properties). Performed the experiments in triplicate (n = 3)

Correlation of total phenolic contents to antioxidant and antidiabetic potency

The quantified total phenolic contents were correlated with percent antioxidant activities of extracted samples and found significant. The non-significant correlation was found between total phenolic contents and antidiabetic potential of samples used (Pearson correlation, two-tailed). Results were articulated in Table 4.

Table 4 The correlation between total phenolic content quantified in methanolic extract of rhizome of Sorghum halepense (L.) Pers and its different soluble fractions and their antioxidant and antidiabetic potency. P-value (two-tailed) and ns: none significant

In vivo study

Test for acute toxicity

A single oral dose of Sorghum halepense (L.) Pers methanol extract (2 g/kg) showed no mortality or acute toxicity symptoms in rats over 24 h. Acute toxicity appraisal has shown that Sorghum halepense (L.) Pers rhizome extract is safe up to 2 g/kg b.w. and the approximate LD50 is more than 2 g/kg.

Effect of a methanol extract of Sorghum halepense (L.) Pers rhizomes on body weight of diabetic rats

The current evaluation indicated a significant (P < 0.05) decline in the weight of diabetic control group with respect to the normal control group on 1st and 21st days of treatment. Treatment of diabetic rats with a methanol extract of Sorghum halepense (L.) Pers rhizomes, reliably (P < 0.05) recovered their body weights with regard to diabetic controls (Fig. 5). The extract at dosage of 300 mg/kg and glibenclamide (10 mg/kg) showed a marked increase in body weight of rats with diabetes on 21st day (Fig. 5).

Fig. 5

Represent the effect of methanolic extract of rhizomes of Sorghum halepense (L.) Pers on body weight of diabetic rats. Represented the data (n = 5) as Mean ± SD, a shows significance at P < 0.001 and c at P < 0.05 (Dunnett’s-test), the normal group was compared with the untreated group and in turn its comparison was made with extract treated group and standard

Effect of methanol extract of Sorghum halepense (L.) Pers rhizomes on blood glucose

Treatment of diabetic rats with Sorghum halepense (L.) Pers rhizome extract expressed significant (p < 0.001) reductions in their blood glucose levels as shown in Fig. 6; thereby expressing the applied extract as a hypoglycemic agent. The diabetic control group demonstrated imperative increases in the concentration of blood glucose during the experiment as compared to the normal control group. Conversely, the rats nourished with a higher dose (300 mg/kg) of extract showed an obvious reduction in the concentration of blood glucose, followed lower dose (150 mg/kg) and standard group (10 mg/kg) as shown in Fig. 6.

Fig. 6

Effect of Sorghum halepense (L.) Pers extract on blood glucose levels of diabetic rat; Represented the data (n = 5) as Mean ± SD, a shows significance at P < 0.001 (Dunnett’s-test), the normal group was compared with untreated group and in turn, its comparison was made with extract treated group and standard

Effect of methanol extract of Sorghum halepense (L.) Pers rhizomes on rat’s liver function

In experimental animals, alloxan attacks the pancreas and also causes damage to other organs such as the kidneys and liver. Serum levels of biochemical markers; AST, ALT, total bilirubin and ALP, which are extremely sensitive to oxidative stress and poisonous chemicals were used to assess liver damage. Protective effects of methanol extract of rhizomes of Sorghum halepense (L.) Pers and glibenclamide against changes in liver serum markers are shown in Table 5. Treatment of rats with alloxan considerably raised the activity of marker enzymes in liver serum, which was significantly recovered (P < 0.05) as compared with the control group after oral administration of Sorghum halepense (L.) Pers rhizome extract. Similarly, the intoxication of alloxan was recovered significantly (p < 0.05) by treatment with glibenclamide (10 mg/kg b.w.).

Table 5 The potency of methanolic extract of rhizomes of Sorghum halepense (L.) Pers to normalize the level of ALT, ALP, AST and total bilirubin in serum

Effect of methanolic extract of Sorghum halepense (L.) Pers rhizomes on triglycerides, LDL cholesterol, total cholesterol, HDL cholesterol and serum VLDL cholesterol

Free radicals cause lipid per-oxidation and affect the lipid profile especially when hepatotoxin react with polyunsaturated fatty acids. In the contemporary evaluation, the levels of serum VLDL cholesterol, triglycerides, HDL cholesterol, total cholesterol and LDL cholesterol are summarized in Table 6. Elevated levels of lipid parameters such as triglyceride, total cholesterol, LDL cholesterol and VLDL cholesterol were present in the serum of diabetic rats, while HDL cholesterol level’s declined. These parameters were reliably recovered depending on the dose in groups treated with the extract (150 and 300 mg/kg of b.w.) of the rhizomes of Sorghum halepense (L.) Pers.

Table 6 Consequences of rhizomes of Sorghum halepense (L.) Pers extract on the concentration of serum’s LDL cholesterol, triglycerides, VLDL cholesterol, total cholesterol and HDL cholesterol

Effect of a methanol extract of Sorghum halepense (L.) Pers rhizomes on urea and creatinine in rat serum

Changes in serum creatinine, total protein and urea concentration in normal, diabetic and treated groups are illustrated in Table 7. Protective effect of rhizomes of Sorghum halepense (L.) Pers extract at concentrations of 150 and 300 mg/kg b.w. was observed where the increased serum level of the renal profile was reliably (p < 0.05) restored; serum creatinine, urea, and a decrease in total serum protein in treatment groups.

Table 7 Consequences of rhizomes of Sorghum halepense (L.) Pers extract on serum level of serum creatinine and urea

Antioxidant activities of a methanol extract of Sorghum halepense (L.) Pers rhizomes in vivo

The present study showed that a methanol extract of Sorghum halepense (L.) Pers rhizomes effectively neutralized alloxan-induced oxidative stress in diabetic rats.

In the normal control (non-diabetic rats), the level of MDA was very low than diabetic control (untreated diabetic rats). The standard, glibenclamide, fairly lessen the level of MDA, while a higher dose (300 mg/kg b.w.) of the plant extract appreciably (P < 0.05) reinstated to an almost normal state. The concentration/level of MDA and glutathione has indirect proportionality; diabetic control (diabetic rats) indicated the highest level of MDA (2.95 ± 0.35 nmol / ml) and the lowest level of glutathione (23.2 ± 3.19 μmol/mg protein) as compared to the normal group. Treatment with the applied extract recovered the glutathione concentration in diabetic rats, since the extract has antioxidant characteristics.

The concentration of superoxide dismutase (SOD) and catalase, antioxidant enzymes, in the liver tissue homogenate decreased to 27.8 ± 4.15 and 21.2 ± 2.16 respectively when compared the diabetic control group with the normal group (SOD: 47.2 ± 4.38, catalase: 36 ± 2.23). The extract treatment caused a significant (P < 0.05) recovery in catalase and SOD levels. The resulting effects of Sorghum halepense (L.) Pers rhizome extract affects the comparative levels of glutathione, MDA, SOD and catalase (Table 8).

Table 8 Consequences of rhizomes of Sorghum halepense (L.) Pers extract on SOD, MDA, glutathione and catalase


Phytochemical components in the plant extracts are considered to be active biologically and are accountable for various actions like antidiabetic, anticancer, antifungal, anti-inflammatory and antibacterial [36, 37]. Two types of metabolites are produced by a plant, primary metabolites i.e. lipids, carbohydrates and proteins and secondary metabolites which include alkaloids, phenolics, terpenes, essential oils, tannins, flavonoids, sterols. The literature study revealed that natural compounds, secondary metabolites played a significant role in the healing of various disorders [38, 39]. Typically, the extraction of the secondary metabolite is based on the polarity of the solvent and its interaction with preferred compounds [40, 41]. The phytochemical analysis of rhizome’s methanolic extract of Sorghum halepense (L.) Pers showed the presence of flavonoids, cardiac glycosides, terpenes, carbohydrates, steroids, alkaloids and proteins. The methanolic extract lacked saponins and gums whereas aqueous and chloroform fractions contained carbohydrates and steroids respectively. On other hand, the cardiac glycosides were missing in the aqueous fraction whilst flavonoids in the chloroform fraction (Table 1). Comparable results were reported in earlier studies of Salix mucronata and Datura metel L [42, 43]. A number of reports on phenolic compounds, like terpenoids and flavonoids showed their strong biological efficacies like antidiabetic, antioxidant and anticancer [44, 45]. The antibacterial, antimalarial, cytotoxic and anticancerous characteristics of alkaloids [46] and Na-K-ATPase inhibitory potential of cardiac glycosides [47] has been reported. Similarly, common phenolic compounds, flavonoids are commonly found in the plants [48] and have antioxidant, antiallergic, antibacterial, antiviral, antineoplastic, antidiarrheal, anti-thrombotic, anti-inflammatory and vasodilatory properties [49]. Furthermore, significant antidiabetic characteristics of the plant based bioactive compounds including phenolic compounds, alkaloids, flavonoids, tannins, terpenoids, glycosides have been reported [50,51,52]. The incidence of the mentioned secondary metabolites in the plant extract and its fractions indicated that Sorghum halepense (L.) Pers may have cytotoxic, antioxidant antidiabetic capabilities associated with different diseases as mentioned in preceding studies [53,54,55]. The cytotoxic capabilities of methanolic extract and its fractions were determined by brine shrimp lethality bioassay. The methanolic extract and its chloroform, aqueous and n-hexane fractions caused brine shrimp lethality up to 70.5 ± 1.2%, 50.4 ± 1.1%, 50.3 ± 1.1% and 40.3 ± 1.6% respectively at the amount of 1000 μg/ml (Table 2). It proposes that the plant extract have credible antimicrobial constituents. Consistent results obtained during the studies of Coscinium blumeanum, Fibraurea tinctoria and Arcangelisia flava [56] and Hapllophyllum tuberculatum [57].

The present results (Table 3) revealed that the methanolic extract has the highest amount of total phenolic content (28.30 ± 1.3 mg GAE/g) followed by chloroform fraction (17.34 ± 1.43 mg GAE/g), an aqueous fraction (12.7 ± 1.32 mg GAE/g) and n-hexane fraction (8.87 ± 1.35 mg GAE/g).

Several existing reports confirm that the plant based extracted and isolated phenolic compounds have phenolic hydroxyl groups; can contribute hydrogen atom or unpaired electron and hence neutralize the free radicals efficiently [58, 59]. The present antioxidant compounds in various plants and even different parts of the same plant have different natures and quantities. Therefore, it is essential to adopt more than one assay to authenticate the antioxidant capability of tested samples [60]. The capabilities of methanolic extract of rhizomes of Sorghum halepense (L.) Pers and its n-hexane, chloroform and aqueous fractions to scavenge free radical was assessed by choosing commonly used standard assay i.e. DPPH, ABTS and H2O2 assays [61]. The free radicals produced during these assays were scavenged by the antioxidant constituents in the plant extract. In DPPH assay, DPPH (a, a-diphenyl-b-picrylhydrazyl) is changed into a, a-diphenyl-b-picrylhydrazine along with alteration in its color indicating the scavenging potential of the plant extract and is measured spectrophotometrically. ABTS assay is suitable for both hydrophilic and lipophilic antioxidants [62]. In this essay, ABTS and potassium persulfate react together to generate a blue chromophore (ABTS•+). The mentioned cation is reduced by reacting with plant extract or standard antioxidant (ascorbic acid) [35]. H2O2 by itself is a puny oxidizing agent but through the oxidation of essential thiol (−SH) groups of enzymes, it can inactivate few enzymes directly. It can enter into the cell by crossing the cell membranes easily. Hydrogen peroxide is catalyzed to hydroxyl radicals and singlet oxygen subject to its exposure to transition metal ions. Singlet oxygen is more toxic to the cellular system than H2O2 by itself. Also the hydroxyl radical may be the cause of its various poisonous effects [63], hence it is vital for cells to control the quantity of H2O2 biologically.

The literature study showed that the aforementioned available compounds in the extract of rhizomes of Sorghum halepense (L.) Pers have the ability to donate hydrogen ions and hence de-colorization of DPPH and ABTS solution [11, 53, 55, 64]. The highest free radicals scavenging potential of methanolic extracts were found 40.02%, 40.48% and 50.85% in DPPH, ABTS and H2O2 assays (Fig. 1, 2 and 3) respectively. The difference in free radical scavenging might be owing to the variations in the number of aromatic rings, nature of hydroxyl groups and molecular weight as well as with the number of active components in the extract and its fractions which change their concentrations by fractionation [54, 55, 64]. The elevated antioxidant capacity of methanolic extract might be owing to more phenolic contents in the mentioned extract as compared to its n-hexane, chloroform and aqueous fractions. The correlation of total phenolic content with the antioxidant activities (Table 4) was found significant. (R2 = 0.8081, 0.81 47 and 0.8023 for DPPH, ABTS and H2O2). These correlations predict that antioxidant characteristic is reliant on phenolic content of a sample. Congruent correlations between antioxidant activities and phenolic contents of different types of sorghum were reported previously [65, 66]. Moreover, strong correlations between phenolic content and antioxidant activities in other cereals such as finger millet and wheat have been documented [67, 68].

Today in the world, diabetes is a major degenerative problem resulting in a number of complications like hypertension, atherosclerosis and microcirculatory disorders [69]. The α-amylase catalyzes the hydrolysis of α-(1, 4)-D-glycosidic linkages of starch and oligosaccharides and liberate monosaccharides in the intestine and thus contribute to hyperglycemia in diabetes. It can be limited by restraining α-amylase in the intestine which slows down the decomposition of starch and oligosaccharides to monosaccharides, reduces assimilation of glucose and consequently decrease postprandial blood glucose level [70]. The mechanism of anti-hyperglycemic potential of extract is unknown. Possibly, it might be due to the presence of flavonoids, terpenes, tannins and alkaloids which might have caused alpha-amylase inhibition. On the basis of their strong binding ability with proteins to form an insoluble and indigestible complexes, they are extensively used as inhibitors [71]. Further, it could elucidate that the phenolics compounds are not the only contributor to antioxidant activity but also causes enzyme inhibition. The α-amylase inhibition might depend on different factors like the methoxy groups, hydroxyl position and lactone rings or the interaction between compounds [72].

The antidiabetic properties of available commercial medicine, glucophage (standard), methanolic extract and chloroform fraction were measured (63.14%), (61.87%) and (22.66%) respectively (Fig. 4). The anti-diabetic capacity of methanolic extract and standard were close and comparable. The percent inhibition of alpha-amylase was found to be dose-dependent. Similar results were also recorded during the in vitro study of Solaria cuspidate leaves [73]. It assumes that the said extract is a valuable source of significant antidiabetic components and was subjected to in vivo study.

The key organ, pancreas determines the energy and dietary state of the body via blood glucose level and secret insulin in response to a raise in blood glucose level [69]. In a situation, where a number of functional beta cells become too limited to produce enough insulin to carry out the body necessities, insulin-dependent diabetes results [74]. Alloxan (beta-cytotoxin), due to its deleterious properties to pancreatic β-cells is renowned for inducing experimental diabetes in several animal species including rats. In this study, alloxan was used to induce diabetes in rats [75] and thereafter treated with different dozes of plant extract and standard drug (glibenclamide). On 21st day of treatment, a substantial (P < 0.01) decline in the body weight of the diabetic control group was observed with respect to normal control group whereas the groups nourished with the methanolic extract of Sorghum halepense (L.) Pers significantly (P < 0.05) reinstated their body weights with respect to diabetic control (Fig. 5). The higher dose (300 mg/kg) of extract indicated considerable development in the weight of diabetic rats at the same interval. Furthermore, comparable increase (P < 0.05) in body weight of glibenclamide treated group was noticed on comparison with diabetic control group (Fig. 5) at the same interval. The restorative effects of Sorghum-tigernut Ibyer extract on changed glucose concentration, tissue and enzyme damages, and loss of weight in diabetic rats was determined by Shiekuma and coworker [76]. The treatment of diabetics rats with methanolic extract of Sorghum halepense (L.) Pers (150 and 300 mg/kg b.w; Fig. 6) for 21 days significantly (P < 0.05) decreased the increased concentration of glucose in serum. This decline in serum glucose level was determined from the difference between the initial and final fasting concentrations of glucose in serum and was compared with diabetic control and reference standard. Comparable findings were found during evaluation of antidiabetic properties of Sorghum bicolor grains [77].

Usually the increase in aminotransferases is a well-known clue of liver dysfunction and is more frequent in diabetic patients than the common population. Moreover, several diabetic complications such as neuropathy, retinopathy and restricted mobility of joints are associated with the function of liver enzymes, regardless of body mass index, alcohol consumption and metabolic control of diabetes [78]. Significant raise in action of several enzymes such as beta-glucuronidase, N-acetyl-beta-glucosaminidase, leucine aminopeptidase and lysosomal acid phosphatase and cathepsin D have been observed following the injection of alloxan in previous studies [79]. Liver dysfunction under diabetic condition leads to increased activities of alkaline phosphatase (ALP), alanine aminotransferase (ALT), total bilirubin and aspartate aminotransferase (AST) with respect to non diabetes (Table 5). In diabetic animals, the alteration of enzymes in serum are directly associated with the metabolic variations wherein these enzymes are concerned. In the lack of insulin, the elevated actions of transaminases are owing to more availability of amino acids in diabetes and are responsible for the increased ketogenesis and gluconeogenesis found in diabetes. During the recent study, the oral feeding of extract (150 and 300 mg/kg b.w.) has restored the concentrations of ALT, total bilirubin, ALP and AST as indicated in Table 5 [80]. Hence, the obvious restoration in the concentration of the mentioned enzymes (Table 5) was the effect of better metabolism of proteins, fats and carbohydrates. Following the treatment, the revival of ALT and bilirubin levels also indicated the recovery of insulin secretion. In alloxan induced diabetic rats, the plant extracts have revealed the restoration of altered levels of ALT, ALP, total bilirubin and AST in earlier studies [81, 82]. Raise in serum urea and creatinine levels owing to diabetic hyperglycemia were considered the significant indicators of renal dysfunction [83, 84] and reveals a decline in the rate of glumerular filtration. The level of creatinine and urea was normalized significantly (P < 0.05) by treating the rats with methanolic extract (Table 7). Harmonious consequences were found in the antidiabetic assessment of three varieties (Heuin sorghum, Chal sorghum and Hwanggeumchal sorghum) from Korean sorghum (Sorghum bicolor L. Monech) [85]. Alloxan induces hypercholesterolemia in diabetics rats, and therefore, evident hyperlipidemia, which shows diabetic state, can be the consequence of inhibition of lipolytic hormones and thus decrease in catalysis of fat deposits [86]. The level of HDL cholesterol, total cholesterol triglycerides, LDL and VLDL cholesterol was considerably recovered by methanolic extract of Sorghum halepense (L.) Pers (Table 6) which shows that it demonstrates hypolipidemic characteristics. The inhibition of the synthesis of fatty acid may lead to lower the level of lipid. Normally insulin triggers lipoprotein lipase which hydrolyses triglycerides whereas in diabetic the owing to a shortage of insulin, inactivation of the mentioned enzyme may result in hypertriglyceridemia [87]. Following the treatment with Sorghum halepense (L.) Pers extract, a remarkable drop-off in serum lipid level in diabetic rats can be directly related to the restoration of insulin level. Analogous findings were achieved during the antidiabetic study of Sorghum and Galium tricornutum extracts [81, 88].

An enzymatic antioxidant protection mechanism can be decreased with an increase in the concentration of lipid peroxidation [89]. In previous studies, the formation of oxygen free radicals in diabetic β cells and its deleterious effects have been documented. The mentioned cells can be saved from oxidative reparation by overexpression of antioxidant enzymes like SOD and CAT [82, 90, 91]. In the liver tissues, significant improvement was observed in the activities of SOD and CAT after treatment with the extract (150 and 300 mg/kg b.w.) during the current study. This recommends that the oxidative stress in diabetes was reduced by the applied extract due to its efficient antioxidant characteristics [90] and can enhance the activities of CAT and SOD [82, 92].

Dysfunction of β-cells and insulin resistance are key markers of diabetes [93] and the later one has a close relationship with altered lipid profile and serves as the major constituent of other metabolic disorders besides diabetes. For example, insulin resistance has been shown to be concerned with diabetic dyslipidemia characterized by high level of VLDL, TG, total cholesterol and low level of HDL [94, 95]. Consequently, the lipid profile is considered in almost all follow-up programs of diabetes and maybe useful for early intervention and hampering the progression of diabetes [93, 96]. After treatment with RSH methanolic extracts, a significant decline in the levels of lipid, ALT and bilirubin in the serum of diabetic rats can be directly related to the restoration of insulin concentration and thereby reduction in blood glucose level. Evaluation of medicinal plants with an aim to find new compounds having therapeutic activities such as antioxidants, hypolipidemic and antidiabetic [97, 98] is an emerging research area.


The current research project suggests that the rhizomes of Sorghum halepense (L.) Pers exhibit significant cytotoxic, antioxidant and anti-diabetic characteristics. The methanolic extract contained higher total phenolic contents than its fractions. Besides, total phenolic contents indicated a significant correlation with antioxidant activities (DPPH, ABTS and H2O2) while non-significant with antidiabetic activity. Finally, it is concluded that the extract of rhizomes of Sorghum halepense (L.) Pers is a valuable source of antioxidant and anti-diabetic compounds. Its further analysis is needed to sharpen its pharmacological activities.



Alkaline phosphatase


Alanine aminotransferase


High density lipoprotein




Low density lipoprotein


Very low density lipoprotein


Superoxide dismutase




sodium dodecyl sulfate


Thiobarbituric acid


Thiobarbituric acid reactive substances




Trichloroacetic acid


Ethylenediamine tetraacetic acid


5, 50-dithiobis-2-nitrobenzoic acid


Nitro blue tetrazolium


  1. 1.

    Singh H, Batish DR, Kohli R. Allelopathy in agroecosystems: an overview. J Crop Prod. 2001;4(2):1–41.

    CAS  Article  Google Scholar 

  2. 2.

    Loddo D, Masin R, Otto S, Zanin G. Estimation of base temperature for Sorghum halepense rhizome sprouting. Weed Res. 2012;52(1):42–9.

    Article  Google Scholar 

  3. 3.

    Rambabu B, Patnaik KR, Srinivas M, Abhinayani G, Sunil J, Ganesh MN. Evaluation of central activity of ethanolic flower extract of Sorghum halpense on albino rats. J Med Plants. 2016;4:104–7.

    Google Scholar 

  4. 4.

    Baerson SR, Dayan FE, Rimando AM, Nanayakkara ND, Liu CJ, Schröder J, Fishbein M, Pan Z, Kagan IA, Pratt LH. A functional genomics investigation of allelochemical biosynthesis in Sorghum bicolor root hairs. J Biol Chem. 2008;283(6):3231–47.

    CAS  PubMed  Article  Google Scholar 

  5. 5.

    Liu Y, Zhang C, Wei S, Cui H, Huang H. Compounds from the subterranean part of Johnsongrass and their allelopathic potential. Weed Biol Manage. 2011;11(3):160–6.

    CAS  Article  Google Scholar 

  6. 6.

    Czarnota MA, Rimando AM, Weston LA. Evaluation of root exudates of seven sorghum accessions. J Chem Ecol. 2003;29(9):2073–83.

    CAS  PubMed  Article  Google Scholar 

  7. 7.

    Sada N, Tanko Y, Mabrouk M. Modulatory role of soya beans supplement on lipid profiles and liver enzymes on alloxan-induced diabetic Wistar rats. Eur J Exp Bio. 2013;3(2):62–7.

    CAS  Google Scholar 

  8. 8.

    Tawfeuk HZ, Hassan NM, Khalil HI, Kerolles SY. Anti-diabetic effects of dietary formulas prepared from some grains and vegetables on type 2 diabetic rats. J Agroaliment Process Technol. 2014;20:69–79.

    Google Scholar 

  9. 9.

    Azeez O, Oyagbemi A, Oyeyemi M, Odetola A. Ameliorative effects of Cnidoscolus aconitifolius on alloxan toxicity in Wistar rats. Afr J Health Sci. 2010;10(3):283–91.

    CAS  Google Scholar 

  10. 10.

    Onuegbu AJ, Olisekodiaka JM, Udo JU, Umeononihu O, Amah UK, Okwara JE, Atuegbu C. Evaluation of high-sensitivity C-reactive protein and serum lipid profile in southeastern Nigerian women with pre-eclampsia. Med Princ Pract. 2015;24(3):276–9.

    PubMed  PubMed Central  Article  Google Scholar 

  11. 11.

    Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;20(7):933–56.

    CAS  PubMed  Article  Google Scholar 

  12. 12.

    Meyer B, Ferrigni N, Putnam J, Jacobsen L, Nichols DJ, McLaughlin JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982;45(5):31–4.

    CAS  PubMed  Article  Google Scholar 

  13. 13.

    McDonald S, Prenzler PD, Antolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem. 2001;73(1):73–84.

    CAS  Article  Google Scholar 

  14. 14.

    Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. Gen Pharmacol. 1999;32:661–7.

    CAS  PubMed  Article  Google Scholar 

  15. 15.

    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9):1231–7.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  16. 16.

    Wettasinghe M, Shahidi F. Scavenging of reactive-oxygen species and DPPH free radicals by extracts of borage and evening primrose meals. Food Chem. 2000;70:17–26.

    CAS  Article  Google Scholar 

  17. 17.

    KWON YI, Apostolidis E, Shetty K. Evaluation of pepper (Capsicum annuum) for management of diabetes and hypertension. J Food Biochem. 2007;31:370–85.

    CAS  Article  Google Scholar 

  18. 18.

    Chinedu E, Arome D. Ameh FS. A new method for determining acute toxicity in animal models. Toxicol Int. 2013;20:224–6.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  19. 19.

    Negreş S, Chiriţă C, Moroşan E, Arsene AL. Experimental pharmacological model of diabetes induction with alloxan in rat. Farmacia. 2013;61(2):313–23.

    Google Scholar 

  20. 20.

    Etuk E. Animals models for studying diabetes mellitus. Agric Biol JN Am. 2010;1(2):130–4.

    CAS  Google Scholar 

  21. 21.

    Kulkarni S. Commonly used drugs, their doses and nature of action in laboratory animals. Handb Exp Pharmacol. 2005;3:190–5.

    Google Scholar 

  22. 22.

    Mule V, Naikwade N, Magdum C, Jagtap V. Antidiabetic activity of extracts of Pithecellobium dulce Benth leaves in alloxan induced diabetic rats. Int J Pharm Sci Drug Res. 2016;8:275–80.

    CAS  Google Scholar 

  23. 23.

    Borgohain R, Lahon K, Das S, Gohain K. Evaluation of mechanism of anti-diabetic activity of Terminalia chebula on alloxan and adrenaline induced diabetic albino rats. Drugs. 2002;19:5.

    Google Scholar 

  24. 24.

    Annida B, Prince PSM. Supplementation of fenugreek leaves reduces oxidative stress in streptozotocin-induced diabetic rats. J Med Food. 2005;8(3):382–5.

    CAS  PubMed  Article  Google Scholar 

  25. 25.

    Suleman S, Alemu T. A survey on utilization of ethnomedicinal plants in Nekemte town, east Wellega (Oromia), Ethiopia. J Hherbs spices med. Plants. 2012;18:34–57.

    Google Scholar 

  26. 26.

    Ben EE, Ekaidem IS. Plasma Insulin and Working dynamics of Calcium Channel blockers on thyroid hormone impaired glucose metabolism. Br J Pharm Res. 2016;13(5):1–8.

    Article  Google Scholar 

  27. 27.

    Spirlandeli A, Deminice R, Jordao A. Plasma malondialdehyde as biomarker of lipid peroxidation: effects of acute exercise. Int J Sports Med. 2014;35(1):14–8.

    CAS  PubMed  Google Scholar 

  28. 28.

    Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351–8.

    CAS  PubMed  Article  Google Scholar 

  29. 29.

    Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta (BBA)-Gen Subj. 1979;582:67–78.

    CAS  Article  Google Scholar 

  30. 30.

    Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44:276–87.

    CAS  PubMed  Article  Google Scholar 

  31. 31.

    Atawodi SE. Evaluation of the hypoglycemic, hypolipidemic and antioxidant effects of methanolic extract of “Ata-Ofa” Polyherbal tea (APolyherbal) in Alloxan-induced diabetic rats. Drug Invent Today. 2011;3(11):70–6.

    Google Scholar 

  32. 32.

    Tupe R, Kemse N, Khaire A. Evaluation of antioxidant potentials and total phenolic contents of selected Indian herbs powder extracts. Int Food Res J. 2013;20(3):1053–63.

    CAS  Google Scholar 

  33. 33.

    Sen S, Chakraborty R, Sridhar C, Reddy Y, De B. Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. Int J Pharm Sci Rev Res. 2010;3:91–100.

    CAS  Google Scholar 

  34. 34.

    Wong SP, Leong LP, Koh JHW. Antioxidant activities of aqueous extracts of selected plants. Food Chem. 2006;99:775–83.

    CAS  Article  Google Scholar 

  35. 35.

    Johnston JW, Dussert S, Gale S, Nadarajan J, Harding K, Benson EE. Optimisation of the azinobis-3-ethyl-benzothiazoline-6-sulphonic acid radical scavenging assay for physiological studies of total antioxidant activity in woody plant germplasm. Plant Physiol Biochem. 2006;44:193–201.

    CAS  PubMed  Article  Google Scholar 

  36. 36.

    Suresh S, Nagarajan N. Antimicrobial activity and preliminary phytochemical analysis of Begonia malabarica Lam. J Pure Appl Microbiol. 2009;3:801–3.

    Google Scholar 

  37. 37.

    Hossain MA, Nagooru MR. Biochemical profiling and Total flavonoids contents of leaves crude extract of endemic medicinal plant Corydyline terminalis L. Kunth. Pharm J. 2011;3(24):765–73.

    Google Scholar 

  38. 38.

    Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol. 2006;71(10):1397–421.

    CAS  PubMed  Article  Google Scholar 

  39. 39.

    Ndam L, Mih A, Fongod A, Tening A, Tonjock R, Enang J, Fujii Y. Phytochemical screening of the bioactive compounds in twenty (20) Cameroonian medicinal plants. Int J Curr Microbiol App Sci. 2014;3(12):768–78.

    Google Scholar 

  40. 40.

    Gonzalez-Guevara JL, Gonzalez-Lavaut JA, Pino-Rodríguez S, Garcia-Torres M, Carballo-Gonzalez MT, Echemendia-Arana OA, Molina-Torres J, Prieto-Gonzalez S. Phytochemical screening and in vitro antiherpetic activity of four Erythtroxylum species. Acta Farm Bonaer. 2004;23:506–9.

    CAS  Google Scholar 

  41. 41.

    Li B, Smith B, Hossain MM. Extraction of phenolics from citrus peels: II. Enzyme-assisted extraction method. Sep Purif Technol. 2006;48(2):189–96.

    CAS  Article  Google Scholar 

  42. 42.

    Alabri THA, Al Musalami AHS, Hossain MA, Weli AM, Al-Riyami Q. Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. J King Saud Univ Sci. 2014;26(3):237–43.

    Article  Google Scholar 

  43. 43.

    El-Sayed MM, El-Hashash MM, Mohamed HR, Abdel-Lateef EES. Phytochemical investigation and in vitro antioxidant activity of different leaf extracts of Salix mucronata Thunb. J Appl Pharm Sci. 2015;5:80–5.

    CAS  Article  Google Scholar 

  44. 44.

    Thitilertdecha N, Teerawutgulrag A, Rakariyatham N. Antioxidant and antibacterial activities of Nephelium lappaceum L. extracts. LWT-Food Sci Technol. 2008;41:2029–35.

    CAS  Article  Google Scholar 

  45. 45.

    Hossain MA, Shah MD, Gnanaraj C, Iqbal M. In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. Asian Pac J Trop Med. 2011;4:717–21.

    CAS  PubMed  Article  Google Scholar 

  46. 46.

    Wirasathien L, Boonarkart C, Pengsuparp T, Suttisri R. Biological activities of alkaloids from Pseuduvaria setosa. Pharm Biol. 2006;44(4):274–8.

    CAS  Article  Google Scholar 

  47. 47.

    Langenhan JM, Peters NR, Guzei IA, Hoffmann FM, Thorson JS. Enhancing the anticancer properties of cardiac glycosides by neoglycorandomization. Proc Natl Acad Sci. 2005;102(35):12305–10.

    CAS  PubMed  Article  Google Scholar 

  48. 48.

    Nijveldt RJ, Van Nood E, Van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001;74(4):418–25.

    CAS  PubMed  Article  Google Scholar 

  49. 49.

    Miller AL. Antioxidant flavonoids: structure, function and clinical usage. Altern Med Rev. 1996;1(2):103–11.

    Google Scholar 

  50. 50.

    Sulyman A, Akolade J, Sabiu S, Aladodo R, Muritala H. Antidiabetic potentials of ethanolic extract of Aristolochia ringens (Vahl.) roots. J Ethnopharmacol. 2016;182:122–8.

    CAS  PubMed  Article  Google Scholar 

  51. 51.

    Sayem A, Arya A, Karimian H, Krishnasamy N, Ashok Hasamnis A, Hossain C. Action of phytochemicals on insulin signaling pathways accelerating glucose transporter (GLUT4) protein translocation. Molecules. 2018;23(2):258.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  52. 52.

    Upadhyay S, Dixit M. Role of polyphenols and other phytochemicals on molecular signaling. Oxidative Med Cell Longev. 2015.

  53. 53.

    Ayoola G, Coker H, Adesegun S, Adepoju-Bello A, Obaweya K, Ezennia E, Atangbayila T. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in southwestern Nigeria. Trop J Pharm Res. 2008;7:1019–24.

    Google Scholar 

  54. 54.

    Anyasor GN, Ogunwenmo O, Oyelana OA, Akpofunure BE. Phytochemical constituents and antioxidant activities of aqueous and methanol stem extracts of Costus afer Ker Gawl.(Costaceae). Afr J Biotechnol. 2010;9(31):4880–4.

    CAS  Google Scholar 

  55. 55.

    Akharaiyi F. Antibacterial, phytochemical and antioxidant activities of Datura metel. Int J PharmTech Res. 2011;1:473–83.

    Google Scholar 

  56. 56.

    Keawpradub N, Dej-adisai S, Yuenyongsawad S. Antioxidant and cytotoxic activities of Thai medicinal plants named Khaminkhruea: Arcangelisia flava, Coscinium blumeanum and Fibraurea tinctoria. Songklanakarin J Sci Technol. 2005;27:455–67.

    Google Scholar 

  57. 57.

    Al-Muniri RMS, Hossain MA. Evaluation of antioxidant and cytotoxic activities of different extracts of folk medicinal plant Hapllophyllum tuberculatum. Egypt J Basic Appl Sci. 2017;4(2):101–6.

    Google Scholar 

  58. 58.

    Casquete R, Castro SM, Martín A, Ruiz-Moyano S, Saraiva JA, Córdoba MG, Teixeira P. Evaluation of the effect of high pressure on total phenolic content, antioxidant and antimicrobial activity of citrus peels. Innov Food Sci Emerg Technol. 2015;31:37–44.

    CAS  Article  Google Scholar 

  59. 59.

    Song FL, Gan RY, Zhang Y, Xiao Q, Kuang L, Li HB. Total phenolic contents and antioxidant capacities of selected Chinese medicinal plants. Int J Mol Sci. 2010;11:2362–72.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  60. 60.

    Kazazic M, Djapo M, Ademovic E. Antioxidant activity of water extracts of some medicinal plants from Herzegovina region. Int J Pure App Biosci. 2016;4(2):85–90.

    Article  Google Scholar 

  61. 61.

    Mathew S, Abraham TE. Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models. Food Chem. 2006;94(4):520–8.

    CAS  Article  Google Scholar 

  62. 62.

    Nenadis N, Wang LF, Tsimidou M, Zhang HY. Estimation of scavenging activity of phenolic compounds using the ABTS•+ assay. J Agric Food Chem. 2004;52(15):4669–74.

    CAS  PubMed  Article  Google Scholar 

  63. 63.

    Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V. Milner a. a novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci. 1993;84:407–12.

    CAS  Article  Google Scholar 

  64. 64.

    Varahalarao V, Kaladhar D. Present scenario of algal-Omics: a mini review. Res Rev. 2012;1(2):8–12.

    Google Scholar 

  65. 65.

    Dykes L, Rooney LW. Sorghum and millet phenols and antioxidants. J Cereal Sci. 2006;44:236–51.

    CAS  Article  Google Scholar 

  66. 66.

    Awika JM, McDonough CM, Rooney LW. Decorticating sorghum to concentrate healthy phytochemicals. J Agric Food Chem. 2005;53(16):6230–4.

    CAS  PubMed  Article  Google Scholar 

  67. 67.

    Adom KK, Sorrells ME, Liu RH. Phytochemical profiles and antioxidant activity of wheat varieties. J Agric Food Chem. 2003;51(26):7825–34.

    CAS  PubMed  Article  Google Scholar 

  68. 68.

    Siwela M, Taylor JR, de Milliano WA, Duodu KG. Occurrence and location of tannins in finger millet grain and antioxidant activity of different grain types. Cereal Chem. 2007;84:169–74.

    CAS  Article  Google Scholar 

  69. 69.

    Edem D. Hypoglycemic effects of ethanolic extracts of alligator pear seed (Persea Americana mill) in rats. Eur J Sci Res. 2009;33:669–78.

    Google Scholar 

  70. 70.

    Kajaria D, Ranjana JT, Tripathi YB, Tiwari S. In-vitro α amylase and glycosidase inhibitory effect of ethanolic extract of antiasthmatic drug—Shirishadi. J Adv. Pharm Technol. 2013;4(4):206–9.

    Google Scholar 

  71. 71.

    Sales PM, Souza PM, Simeoni LA, Magalhães PO, Silveira D. α-amylase inhibitors: a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci. 2012;15(1):141–83.

    PubMed  Article  Google Scholar 

  72. 72.

    Quan NV, Xuan TD, Tran H-D, Thuy NTD, Trang LT, Huong CT, Andriana Y, Tuyen PT. Antioxidant, α-amylase and α-glucosidase inhibitory activities and potential constituents of Canarium tramdenum bark. Molecules. 2019;24(3):605.

    PubMed Central  Article  CAS  PubMed  Google Scholar 

  73. 73.

    Manikandan R, Anand AV, Kumar S. Phytochemical and in vitro Antidiabetic activity of Psidium Guajava leaves. Pharm J. 2016;8:4.

    Google Scholar 

  74. 74.

    Ibeh BO, Ezeaja MI. Preliminary study of antidiabetic activity of the methanolic leaf extract of Axonopus compressus (P. Beauv) in alloxan-induced diabetic rats. J Ethnopharmacol. 2011;138:713–6.

    PubMed  Article  Google Scholar 

  75. 75.

    Rerup CC. Drugs producing diabetes through damage of the insulin secreting cells. Pharmacol Rev. 1970;22(4):485–518.

    CAS  PubMed  Google Scholar 

  76. 76.

    Shiekuma S, Ukeyima M, Msendoo JA, Blessing I, Tughgba T. Effect of Sorghum-tigernut Ibyer (a traditional gruel) on the fasting blood glucose levels of Alloxan-induced diabetic rats. Eur J Nutr Food Safety. 2019;1:260–8.

    Article  Google Scholar 

  77. 77.

    Olawole TD, Okundigie MI, Rotimi SO, Okwumabua O, Afolabi IS. Preadministration of fermented sorghum diet provides protection against hyperglycemia-induced oxidative stress and suppressed glucose utilization in alloxan-induced diabetic rats. Fronti Nutr. 2018;5:16.

    CAS  Article  Google Scholar 

  78. 78.

    Arkkila PE, Koskinen PJ, Kantola IM, Rönnemaa T, Seppänen E, Viikari JS. Diabetic complications are associated with liver enzyme activities in people with type 1 diabetes. Diabetes Diabetes Res Clin Pract. 2001;52(2):113–8.

    CAS  PubMed  Article  Google Scholar 

  79. 79.

    Witek B, Krol T, Kolataj A, Ochwanowska E, Stanislawska I, Slewa A. The insulin, glucose and cholesterol level and activity of lysosomal enzymes in the course of the model alloxan diabetes. Neuroendocrinol Lett. 2001;22:238–42.

    CAS  PubMed  Google Scholar 

  80. 80.

    Mori DM, Baviera AM, de Oliveira Ramalho LT, Vendramini RC, Brunetti IL, Pepato MT. Temporal response pattern of biochemical analytes in experimental diabetes. Biotechnol Appl Biochem. 2003;38:183–91.

    CAS  PubMed  Article  Google Scholar 

  81. 81.

    Khan ST, Ahmed M, Khan RA, Mushtaq N, Khan N. Anti-diabetic potential of aerial parts of Galium tricornutum (Dandy) Rubiaceae. Trop J Pharm Res. 2017;16:1573–8.

    CAS  Article  Google Scholar 

  82. 82.

    Rajaram K. Antioxidant and antidiabetic activity of Tectona grandis Linn. In alloxan induced Albino rats. Drugs. 2013;4:174–7.

    Google Scholar 

  83. 83.

    Saeed MK, Deng Y, Dai R. Attenuation of biochemical parameters in streptozotocin-induced diabetic rats by oral administration of extracts and fractions of Cephalotaxus sinensis. J Clin Biochem Nutr. 2008;42:21–8.

    CAS  PubMed  Article  Google Scholar 

  84. 84.

    Doi K, Kurabe S, Shimazu N, Inagaki M. Systemic histopathology of rats with CCl4-induced hepatic cirrhosis. Lab Anim. 1991;25:21–5.

    CAS  PubMed  Article  Google Scholar 

  85. 85.

    Chung IM, Kim E-H, Yeo MA, Kim SJ, Seo MC, Moon HI. Antidiabetic effects of three Korean sorghum phenolic extracts in normal and streptozotocin-induced diabetic rats. Food Res Int. 2011;44:127–32.

    CAS  Article  Google Scholar 

  86. 86.

    Fernandes NP, Lagishetty CV, Panda VS, Naik SR. An experimental evaluation of the antidiabetic and antilipidemic properties of a standardized Momordica charantia fruit extract. BMC Complement Altern Med. 2007;7(29).

  87. 87.

    Antidiabetic KRA. Antioxidant, and Hypolipidemic potential of Sonchus asper hill. Altern Ther Health Med. 2017;23(7):34–40.

    Google Scholar 

  88. 88.

    Kim J, Park Y. Anti-diabetic effect of sorghum extract on hepatic gluconeogenesis of streptozotocin-induced diabetic rats. Nutr Metab. 2012;9(1):106.

    Article  Google Scholar 

  89. 89.

    Nizamutdinova IT, Jin YC, Chung JI, Shin SC, Lee SJ, Seo HG, Lee JH, Chang KC, Kim HJ. The anti-diabetic effect of anthocyanins in streptozotocin-induced diabetic rats through glucose transporter 4 regulation and prevention of insulin resistance and pancreatic apoptosis. Mol Nutr Food Res. 2009;53:1419–29.

    CAS  PubMed  Article  Google Scholar 

  90. 90.

    Cemek M, Kağa S, Şimşek N, Büyükokuroğlu ME, Konuk M. Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin-induced diabetic rats. J Nat Med. 2008;62:284–93.

    PubMed  Article  Google Scholar 

  91. 91.

    Adewole S, Ojewole J. Protective effects of Annona muricata Linn.(Annonaceae) leaf aqueous extract on serum lipid profiles and oxidative stress in hepatocytes of streptozotocin-treated diabetic rats. Afr J Tradit Complement Altern Med. 2009;6(1):30–41.

    Google Scholar 

  92. 92.

    Bagri P, Ali M, Aeri V, Bhowmik M, Sultana S. Antidiabetic effect of Punica granatum flowers: effect on hyperlipidemia, pancreatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes. Food Chem Toxicol. 2009;47:50–4.

    CAS  PubMed  Article  Google Scholar 

  93. 93.

    Goldstein BJ. Insulin resistance as the core defect in type 2 diabetes mellitus. The Am J Card. 2002;90(5):3–10.

    Article  Google Scholar 

  94. 94.

    Santos-Gallego CG, Rosenson RS. Role of HDL in those with diabetes. Curr Cardiol Rep. 2014;16(9):512.

    Article  PubMed  Google Scholar 

  95. 95.

    Mooradian AD. Dyslipidemia in type 2 diabetes mellitus. Nat Rev Endocrinol. 2009;5(3):150–9.

    CAS  Article  Google Scholar 

  96. 96.

    Li N, Fu J, Koonen DP, Kuivenhoven JA, Snieder H, Hofker MH. Are hypertriglyceridemia and low HDL causal factors in the development of insulin resistance? Atherosclerosis. 2014;233(1):130–8.

    CAS  PubMed  Article  Google Scholar 

  97. 97.

    Vijayalakshmi R, Ravindhran R. Pharmacognostical studies on root of Diospyrus ferreae (Willd.) Bakh and Aerva lanata Linn. A potent Indian medicinal plants. Asian J Pharm Clin Res. 2013;6:57–62.

    Google Scholar 

  98. 98.

    Rout SP, Kar DM, Mohapatra SB, Swain SP. Anti-hyperglycemic effect Annona reticulata L. leaves on experimental diabetic rat model. Asian J Pharm Clin Res. 2013;6:56–60.

    Google Scholar 

Download references


I am grateful to all my colleagues and friends and who guided me.

Disclosure statement

The author declares that he/she has no conflict of interests.


No funding is received for conduction of the study.

Author information




MAS collected plant, conducted experiments, collected and analyzed data and drafting of the manuscript. RAK (ORCID ID: 0000–0003–0453-2090) and MA has made significant influence to onset and design, analysis of data and drafting of manuscript. The authors read and approved the final manuscript.

Corresponding author

Correspondence to Rahmat Ali Khan.

Ethics declarations

Ethics approval and consent to participate

The study was conducted according to the protocol approved by Institutional Animals Ethics Committee (Ref. No. University of Science and Technology Bannu/ Biotech/Ethical/123) of the University.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Shah, M.A.R., Khan, R.A. & Ahmed, M. Sorghum halepense (L.) Pers rhizomes inhibitory potential against diabetes and free radicals. Clin Phytosci 7, 19 (2021).

Download citation


  • Sorghum halepense (L.) Pers
  • Antioxidant
  • Cytotoxic and anti-diabetic