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International Journal of Phytomedicine and Phytotherapy

Table 2 Calibration curves, linear range, and detection and quantification limits of CCAE phytochemical analysis

From: Differential expression of gluconeogenic enzymes in early- and late-stage diabetes: the effect of Citrullus colocynthis (L.) Schrad. Seed extract on hyperglycemia and hyperlipidemia in Wistar-Albino rats model

Phytochemical

Peak number

Mg/g freeze-dried Extract

Correlation

LR (μg/ml)

LOD (μg/ml)

LOQ (μg/ml)

Total Phenolic Content (GAE)

10.43 ± 3.09

y = 0.0058x + 0.0329 R2 = 0.9987

0–125

1.92

5.83

Total Flavonoids (CE)

14.65 ± 0.94

y = 2.164x + 0.0994 R2 = 0.9977

0–1250

58.56

177.47

Free Radical Scavenging (GAE)

4.67 ± 0.55

y = 2.4796x + 6.4969 R2 = 0.9897

0.-3125

2.04

6.20

Total Antioxidant Capacity (GAE)

158.40 ± 15.7

y = 0.1755x + 0.0345 R2 = 0.9932

0–5000

405.51

1228.83

Rutin hydrate (RH)

10

17.67 ± 1.66

y = 24,133x – 55,464 R2 = 0.9999

0.12–500

3.87

11.74

Ferulic acid (Fer A)

7

0.81 ± 0.48

y = 93,329x + 149,430 R2 = 0.9999

0.12–500

3.45

10.46

  1. GAE Gallic acid equivalent, CE Catechin equivalent. The concentrations of GA, RH, Fer A, and Chl A are the average of three readings and after adjusting for their recoveries at around the CCAE peak area for each compound and pertain to Fig. 1A