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International Journal of Phytomedicine and Phytotherapy

Table 2 Calibration curves, linear range, and detection and quantification limits of CCAE phytochemical analysis

From: Differential expression of gluconeogenic enzymes in early- and late-stage diabetes: the effect of Citrullus colocynthis (L.) Schrad. Seed extract on hyperglycemia and hyperlipidemia in Wistar-Albino rats model

Phytochemical Peak number Mg/g freeze-dried Extract Correlation LR (μg/ml) LOD (μg/ml) LOQ (μg/ml)
Total Phenolic Content (GAE) 10.43 ± 3.09 y = 0.0058x + 0.0329 R2 = 0.9987 0–125 1.92 5.83
Total Flavonoids (CE) 14.65 ± 0.94 y = 2.164x + 0.0994 R2 = 0.9977 0–1250 58.56 177.47
Free Radical Scavenging (GAE) 4.67 ± 0.55 y = 2.4796x + 6.4969 R2 = 0.9897 0.-3125 2.04 6.20
Total Antioxidant Capacity (GAE) 158.40 ± 15.7 y = 0.1755x + 0.0345 R2 = 0.9932 0–5000 405.51 1228.83
Rutin hydrate (RH) 10 17.67 ± 1.66 y = 24,133x – 55,464 R2 = 0.9999 0.12–500 3.87 11.74
Ferulic acid (Fer A) 7 0.81 ± 0.48 y = 93,329x + 149,430 R2 = 0.9999 0.12–500 3.45 10.46
  1. GAE Gallic acid equivalent, CE Catechin equivalent. The concentrations of GA, RH, Fer A, and Chl A are the average of three readings and after adjusting for their recoveries at around the CCAE peak area for each compound and pertain to Fig. 1A