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Clinical Phytoscience

International Journal of Phytomedicine and Phytotherapy

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In vitro antimalarial activity evaluation of two ethnomedicinal plants against chloroquine sensitive and resistant strains of Plasmodium falciparum

Clinical Phytoscience volume 7, Article number: 42 (2021) Cite this article

Abstract

Background

In this study, we selected two medicinal plants Citrus maxima (Burm.) Merr. and Artemisia nilagirica (C.B. Clarke) Pamp. on the basis of their traditional use in the treatment of fever associated with malaria in Assam (India) and evaluated their antimalarial potential against Plasmodium falciparum strains.

Methods

The properly processed plant parts of C. maxima (Burm.) Merr. and A. nilagirica (C.B. Clarke) Pamp. were extracted with different solvents from nonpolar to polar by cold maceration technique. After that antimalarial activities of the extracts were evaluated against both chloroquine sensitive (3D7) and resistant (RKL-9) strains of P. falciparum using Giemsa staining light microscopy technique. The most active extract(s) was further screened for cytotoxicity potential against murine macrophage RAW264.7 cell line using MTT assay. Then preliminary phytochemical screening and qualitative fingerprint analysis of the active extract(s) were done to check the presence of different secondary metabolites.

Results

From the in vitro study, the hydro-alcoholic extract of C. maxima (Burm.) Merr. and methanol extract of A. nilagirica (C.B. Clarke) Pamp. were found to be the most active against both 3D7 and RKL-9 strains. In the cytotoxicity study, the CC50 values of the active extracts were found to be > 100 μg/ml, which suggested the safety of the extracts. Then phytochemical and fingerprint analysis revealed the presence of various important plant secondary metabolites in both the extracts.

Conclusion

The findings of this study confirmed the presence of antimalarial potential of hydro-alcoholic extract of C. maxima (Burm.) Merr. and methanol extract of A. nilagirica (C.B. Clarke) Pamp without having any toxic effect. Both the extracts showed IC50 values below 5 μg/ml against 3D7 and RKL-9 strains.

Background

Different approaches are adopted for the development of new drugs against malaria, where natural compound-based approach is one of the preferable one. It is to mention that several important drug candidates available in the market were originated from plant sources [1, 2]. The antimalarial drugs, quinine and artemisinin were also isolated from the plant sources based on the information of traditional use [3]. Later several potent semisynthetic and synthetic derivatives of quinine and artemisinin were developed, some of which are still in clinical use and some new derivatives are under developmental stages [4]. Scientists from different regions including India are still searching for the potent antimalarial compounds from various natural resources and have found some plants having antimalarial activity [5]. Being a part of Sub-Himalayan region, North East (NE) India is rich in plant diversity and various plants of this region have been using in traditional medicine by various ethnic groups of the region. In this study, we selected two ethnomedicinal plants, Citrus maxima (Burm.) Merr. and Artemisia nilagirica (C.B. Clarke) on the basis of their use in the preparation of antimalarial herbal remedy in Assam, a state of NE India. Citrus maxima (Burm.) Merr., commonly known as ‘Pumelo’ has diverse biological activities like antioxidants, antihyperglycemic/antidiabetic, hepatoprotective, anticancer, antimicrobial/antibacterial, antidepressant, antiaging, cholinesterase and tyrosine inhibition etc. [6,7,8,9,10,11,12,13,14]. Besides it was also reported that the leaves and fruit parts of C. maxima is also used in the treatment of malaria in Benin which is a highly malaria affected West African region [15]. From the fruit part of Citrus maxima (Burm.) Merr. our group already reported one compound luteolin as a potent antimalarial lead compound against P. falciparum using in silico and in vitro analysis [16]. On the other hand, A. nilagirca is reported to have several important biological activities like antioxidant, cytoprotective, anti-inflammatory, antiproliferative, antibacterial, anticancer etc. [17,18,19,20,21,22,23,24,25]. Apart, this plant also possesses larvicidal, pupicidal, adulticidal and mosquito repellant activity [26,27,28]. Besides Panda et al. evaluated the antimalarial potency of the different extracts of Artemisia nilagirica (C.B. Clarke) against an African strain (FCR-3) of P. falciparum using in vitro technique and found IC50 value 5.76 ± 0.82 μg/ml of the methanol extract [29]. Based on the previous reports and their use in traditional medicine, it was hypothesized to have active phytoconstituent(s) against the resistant strains of malaria parasites. Hence, in this study, we investigated the antimalarial potency of different extracts of C. maxima and A. nilagirica against chloroquine sensitive (3D7) and chloroquine resistant (RKL-9) strains of P. falciparum.

Methods

Chemicals and reagents

The analytical reagent grade (EMPARTA ACS grade) solvents like petroleum ether (40–60 °C), toluene, chloroform, ethyl acetate, methanol, ethanol and analytical TLC aluminium plates were purchased from Merck Millipore, Burlington, Massachusetts, USA. The different analytical grade (AR) chemicals like sodium bicarbonate, HEPES buffer, D-glucose, D-sorbitol, formic acid, phosphate buffer solution 10X (PBS), Giemsa stain, sodium pyruvate, dimethyl sulfoxide (DMSO) and plastic wares used in the biological activity screening were purchased from HiMedia Pvt. Ltd., Mumbai, India. Molecular biology grade Fetal bovine serum (FBS), gentamycin, amphotericin-B, penicillin, streptomycin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), RPMI-1640 (Roswell Park Memorial Institute-1640), DMEM (Dulbecco’s Modified Eagle’s medium) were purchased from Gibco-BRL, Life Technologies Inc., Gaithersburg, MD 20884–9980, USA and Sigma Aldrich, St. Louis, MO, USA. The marker compound and standard drug, namely, quercetin (HPLC grade ≥ 95%) and chloroquine phosphate (Certified Reference Material grade) were purchased from Sigma-Aldrich, St. Louis, MO, USA.

Plant materials

The information regarding the use of different plants as herbal remedies for the treatment of fever associated with malaria was collected by interviewing local practitioners in the form of questionnaires and from local books.

Herbariums of the two selected plants (Voucher specimen no. DU/DRS/HRB/NG/2018–19/FS-01 and DU/DRS/HRB/NG/2018–19/FS-05) were prepared according to standard procedure and sent to Botanical Survey of India, Shillong for identification and authentication. Then the plant parts used in the preparation the traditional herbal remedy were collected and processed according to the guideline of Good Agricultural and Collection Practice of WHO [30]. The processed plant materials were coarsely powdered with a mechanical grinder and stored in airtight containers.

Extraction of the plant materials

The extraction of the powdered plant materials was carried out using cold maceration technique using petroleum ether (40–60 °C), chloroform, ethyl acetate, methanol and hydro-alcoholic (1:1) solvent system. Besides, one sample from each plant were prepared as per the traditional practices. In the cold maceration, about 500 g plant material was taken with 3 l of solvent and kept for 72 h with occasional shaking [31]. After that, the solvents were removed under reduced pressure at low temperature using a rotary evaporator (IKA Rotary Evaporator RV 8 V). In the case of the hydro-alcoholic extract, the water part was removed by lyophilisation of the sample using laboratory freeze dryer (IIC Industrial Corporation). The extracts were preserved in glass sample bottles and kept in − 20 °C for further use. The extracts were prepared at ratio of 1:6 during cold maceration process. The yields of the extracts of C. maxima were found to be 0.262%, 0.562%, 2.882%, 1.151%, 8.305% and 0.167% w/w with petroleum ether (40–60 °C), chloroform, ethyl acetate, methanol, hydro-alcoholic and traditional solvent system respectively. Similarly, in case of A. nilagirica, the yields of the extracts were found to be 0.757%, 1.627%, 1.891%, 2.062%, 1.770% and 0.215% w/w with petroleum ether (40–60 °C), chloroform, ethyl acetate, methanol, hydro-alcoholic and traditional solvent system respectively.

In vitro antimalarial screening of the extracts

Preparation of standard and test samples

For this study, 1 mg/ml stock solutions of the extracts were prepared by using incomplete RPMI-1640 media containing 0.5% DMSO. Chloroquine phosphate was used as standard drug in this study and prepared 100 μg/ml stock using the same protocol as used for extracts for further use.

In vitro culture of malaria parasites

The chloroquine sensitive (3D7) and chloroquine resistant (RKL-9) strains of the malaria parasite P. falciparum were obtained from the Parasite Bank of National Institute of Malaria Research (Indian Council of Medical Research), New Delhi. The strains of P. falciparum were maintained in fresh A+ erythrocytes suspended in RPMI-1640 medium supplemented with 25 mM HEPES, 1% D-glucose, 0.23% sodium bicarbonate, gentamycin (40 mg/ml), amphotericin-B (0.25 mg/ml) and 10% heat-inactivated AB+ serum at 37 °C and 5% CO2 environment [32, 33]. After every 24 h, the used medium was replaced with fresh medium supplemented with 10% heat-inactivated AB+ serum and the parasitemia level was maintained below 2%.

Antimalarial activity screening & determination of IC50 values

The antimalarial screening of the extracts was carried out against both 3D7 and RKL-9 strain of P. falciparum by Giemsa staining light microscopy method. For antimalarial testing, initially the asynchronous P. falciparum parasites were synchronized to obtain only the ring stage parasitized cells by treating with 5% D-sorbitol [34]. The initial ring stage parasitemia was maintained at 0.5% in 4% haematocrit using complete medium and fresh A+ erythrocytes before using in the screening. For screening, test and standard drugs were taken in nine different concentrations by two fold serial dilutions for both the 3D7 and RKL-9 strains in 96 well plates. The concentration ranges for test drug and standard drug were 50 to 0.19 μg/ml and 5 to 0.019 μg/ml respectively. All the treatments were performed in triplicates. Then parasitized blood was added to the wells of 96-well plate containing 100 μl of test and standard samples to carry out the assay. The plates were incubated at 37 °C in an environment of 5% CO2 for 36–40 h in a CO2 incubator. After the incubation period, blood smears were prepared in glass slide from each well and fixed by treating with methanol. The slides were stained with 10% Giemsa stain prepared in 1% phosphate buffer solution (PBS). After that number of schizonts (3 or more merozoites containing) per 100 asexual parasites were counted under a light microscope (Leica DM1000) at 1000X (oil emersion) magnification [35]. The percentage inhibition for each concentration was calculated by the following equation:

$$ \% Inhibition=1-\frac{no. of\ schizonts\ in\ test}{no. of\ schizonts\ in\ negative\ control}\times 100 $$

Finally, the IC50 values were calculated by plotting nonlinear regression curve between log dose vs percentage (%) inhibition using GraphPad Prism (GraphPad Prism v.7 San Diego, California, USA). Based on the obtained IC50 values, the extracts were categorized in to active (< 10 μg/ml), intermediate (10–25 μg/ml) or inactive (> 25 μg/ml) categories [36].

In vitro cytotoxicity study

The most active extract obtained for each plant from the in vitro antimalarial study were further taken for in vitro cytotoxicity study using MTT assay [37]. The study was carried out against normal murine macrophage RAW264.7 cell line. Approximately, 1 × 104 cells/ml were cultured in DMEM (Dulbecco’s Modified Eagle’s medium) media supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 10% FBS (Fetal Bovine Serum), penicillin (100 units/ml), streptomycin (10 μg/ml) and allowed to incubate at 37 °C in a humidified 5% CO2 environment. After 80% cell confluency, the cells were treated with different concentrations (50, 100, 200 and 500 μg/ml) of the selected test extracts and incubated for 24 h. Cells without any treatment were considered as control. After 24 h incubation, 0.5 mg/ml MTT was added to each well of the plates and incubated for a further 5 h. After the completion of the incubation, formed formazone complexes were dissolved properly in MTT solvent and the absorbance was taken at 570 nm using a microplate reader (Multiskan™ FC Microplate Photometer). The experiment was performed in triplicates and the percentage of cell viability was calculated for each concentration and compared to control cells without any treatment. Finally, CC50 (cytotoxic concentration) were determined for the test extracts.

Phytochemical and qualitative fingerprint analysis

The various biological activities shown by plant extracts are mainly due to the presence of the different secondary metabolites like alkaloids, flavonoids, glycosides, terpenoids, saponins, steroids, tannins etc. [38]. The active extract from each plant was analysed by chemical reagents to detect the presence of those secondary metabolites using standard procedures as described in the earlier study [39].

The qualitative fingerprint analysis of an extract under the controlled environment is one of the quality control parameters for herbal products [40, 41]. In this study, the qualitative fingerprints of the active extracts were developed using HPTLC densitometry analysis under control environment of temperature and humidity. The study was carried out by using Camag TLC Scanner 4, semiautomatic sample spotter Linomat 5, UV visualization cabinet (deuterium, tungsten and mercury lamp), 100 μl Hamilton dosage syringe, TLC aluminium plate pre-coated with silica gel 60 F254 (10 cm × 10 cm) and glass twin trough chamber (10 cm × 10 cm).

Initially, stock solutions of the marker compound (quercetin for A. nilagirica)/semi-purified fraction for C. maxima (1 mg/ml) and active extracts (10 mg/ml) were prepared in their respective solvents by sonicating for 15 min. Then the samples were centrifuged at 2000 rpm for 5 min and the supernatants were transferred to the sample vials by filtration for further use.

The experiment was carried out at a temperature of 25 ± 2 °C, relative humidity 55%. In the TLC plate (10 cm × 10 cm), 2 μL of the marker compound or semi-purified fraction in duplicate and 4 μL of the active extracts in quadruplicate were applied as a band of 8 mm × 1 mm in size at a distance of 8 mm from the bottom. After applying the sample, the plates were developed using 10 ml mobile phase composed of toluene: ethyl acetate: methanol: formic acid at a ratio of 3:5:1:0.5 using the glass twin trough chamber (10 cm × 10 cm). The plates were developed up to a distance of 7 cm and air-dried at room temperature. Then plates were visualized under UV cabinet at 254 nm and 366 nm. After that, the plates were scanned at 254 nm in absorbance mode and at 366 nm in fluorescence mode using the Camag TLC scanner 4 linked with VisionCAT 2.5 software. During scanning, the slit dimension was kept at 5 × 0.2 mm and the scanning speed was employed at 20 mm/s [42].

Results

Identification and small scale extraction of plant materials

The plants used in the preparation of herbarium were identified as Citrus maxima (Burm.) Merr. (F: Rutaceae) and Artemisia nilagirca (C.B. Clarke) Pamp. (F: Asteraceae) by Dr. N. Odyuo, Scientist D, Botanical Survey of India, Shillong (Ref no. BSI/ERC/Tech/2019/481 dated 23.09.2019). After completing the extraction process, the highest yield was obtained in hydro-alcoholic solvent system for C. maxima whereas highest yield was obtained in methanol solvent for A. nilagirica.

In vitro antimalarial activity

The slides prepared from each well were observed under the light microscope and the number of infected erythrocytes were counted within a particular area. From the infected erythrocytes, the number of parasites with schizont stage were calculated and used for determination of % inhibition. The in vitro antimalarial activity of the extracts was determined in the form of IC50 values using non-linear regression analysis against both 3D7 and RKL-9 strains, and compared with the standard drug chloroquine phosphate (Table 1 & Fig. 1). The representative photomicrographs of smear observed under the microscope during counting are shown in Fig. 2. From the results, it was observed that in the case of C. maxima the hydro-alcoholic extract (CM-HA) showed the lowest IC50 values 3.41 ± 0.31 μg/ml and 4.45 ± 0.10 μg/ml against 3D7 and RKL-9 strains respectively. In the case of A. nilagirica, methanol extract (AN-ME) showed the lowest IC50 values 3.28 ± 0.08 μg/ml and 3.81 ± 0.34 μg/ml against 3D7 and RKL-9 stains respectively.

Table 1 IC50 values of the plant extracts against 3D7 and RKL-9 strains of P. falciparum
Full size table
Fig. 1
figure 1

Calculation of IC50 values by non-linear regression analysis (GraphPad Prism) using nine different concentrations with two-fold serial dilution. For the test extracts the dilution was made from 50 μg/ml to 0.195 μg/ml whereas for the standard drug the dilution was made from 5 μg/ml to 0.019 μg/ml. The experiment was performed in triplicates and each value is the mean ± SD of three replicates

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Fig. 2
figure 2

Representative photomicrographs taken during the observation under light microscope at 1000X (oil emersion) magnification showing infected erythrocytes by different strains of P. falciparum; a. 3D7 without treatment, b. 3D7 with treatment (50 μg/ml), c. RKL-9 without treatment and d. RKL-9 with treatment (50 μg/ml)

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In vitro cytotoxicity study

The active extract(s) selected from the antimalarial screening were further analysed for cytotoxicity potential by MTT assay. The methanol extract of A. nilagirica (AN-ME) and hydro-alcoholic extract of C. maxima (CM-HA) were screened against normal murine macrophage RAW264.7 cell line where % viability for the methanol extract of A. nilagirica was found to be more than 90% at the maximum dose 500 μg/ml (Fig. 3). On the other hand, the % viability for the hydro-alcoholic extract of C. maxima was found to be less than 50% at the maximum dose of 500 μg/ml (Fig. 3). Later CC50 values for both the extracts were determined and found to be more than 100 μg/ml for both the extracts (Fig. 3).

Fig. 3
figure 3

Cytotoxic effects of the active extracts of C. maxima and A. nilagirica on murine macrophage RAW264.7 cell line; a Cell viability after treatment of 24 h with the different dose of the extracts. b Determination of CC50 values of the two extracts (CM-HA and AN-ME). The experiment was performed in triplicates and each value is the mean ± SD of three replicates

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Phytochemical & fingerprint analysis

The bioactive potential shown by a plant extract is due to the presence of different plant secondary metabolites in the sample. From the preliminary chemical tests, alkaloids, flavonoids and glycosides were found to be present in the CM-HA extract whereas, alkaloids, flavonoids, tannins and terpenoids were found to be present in the AN-ME extract (Table S1).

The qualitative fingerprint analysis of extract is a standardization process of plant material or plant extract in the absence of known marker or standard compound. The fingerprints of the active extracts were developed under control environmental conditions like temperature and humidity, and found the presence of various types of compounds in the extracts (Figs. 4 and 5). The chromatograms developed after scanning under 254 nm (absorbance mode) and 366 nm (fluorescence mode) for each extract revealed the presence of multiple components (based on number of peaks) and their possible quantity (based on the area of the peaks) (Fig. S1 & S2).

Fig. 4
figure 4

Visualization of the TLC plates under UV visualizer at 254 nm and 366 nm to detect the presence of different plant secondary metabolites of Citrus maxima (Burm.) Merr

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Fig. 5
figure 5

Visualization of the TLC plates under UV visualizer at 254 nm and 366 nm to detect the presence of different plant secondary metabolites of Artemisia nilagirica (C.B. Clarke) Pamp

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Discussion

The discovery of new antimalarial lead molecule(s) or the development to of antimalarial phytopharmaceutical product(s) from traditionally used plant materials/parts has gained significant importance [43]. For that purpose, the preliminary in vitro antimalarial screening of the plant materials/parts is very essential along with their toxicity assessment against normal mammalian/human cell. Therefore, this study aimed to evaluate the antimalarial potential of the different extracts of the two selected traditionally used plants as well as cytotoxic effect of the active extracts in in vitro conditions. All the extracts obtained from the two plants (C. maxima and A. nilagirica) showed the antimalarial activity within different ranges in the form of IC50 values in in vitro antimalarial analysis. From the results, we found that the ethyl acetate, methanol and hydro-alcoholic extracts came under active category (< 10 μg/ml) against both 3D7 and RKL-9 strains of P. falciparum. The chloroform extract of C. maxima also came under the active category only against the 3D7 strain of P. falciparum. However, among the active extracts, in the case of C. maxima, the hydro-alcoholic extract showed the best activity with IC50 values 3.41 ± 0.31 μg/ml and 4.45 ± 0.10 μg/ml against 3D7 and RKL-9 respectively (Table 1). Whereas in the case of A. nilagirica, the methanol extract showed the best activity with IC50 values 3.28 ± 0.08 μg/ml (3D7) and 3.81 ± 0.34 μg/ml against 3D7 and RKL-9 respectively (Table 1). These two extracts may contain the phytoconstituents which are responsible for strong antimalarial activity against P. falciparum strains. The antimalarial activity of the root part of C. maxima is not reported till date. But the antimalarial activity of the leaves of A. nilagirica was reported by Panda et al. against a different strain of P. falciparum where methanol extract was found to be the most active [29]. The preliminary phytochemical screening of the extracts revealed the presence of some important class of plant secondary metabolites like alkaloids, flavonoids and terpenoids. Pan et al., 2018 already reported the antimalarial activity of secondary metabolites like alkaloids, flavonoids and terpenoids [44]. Hence, the potential in vitro antimalarial activity showed by the active extracts (CM-HA and AN-ME) against the two P. falciparum strains may be due to the presence of these important classes of secondary metabolites. Although the IC50 values of these two extracts were not near to the IC50 values of the standard drug chloroquine phosphate (IC50 values 0.54 ± 0.02 μg/ml and 0.88 ± 0.04 μg/ml against 3D7 and RKL-9 respectively), at extract level the results were satisfactory and can be taken for further study [45]. In the case of both the plants, the samples prepared in the traditional way were found to be either in the intermediate or inactive category. But as they were showing some extent of inhibition in the growth of the parasites hence the traditional way of using these plant parts give some benefits to the patients. The activity of the other extracts also suggests the positive impacts of these plants on the treatment of malaria.

The two active extracts were further examined for their cytotoxicity potential against normal cell line (murine macrophage RAW264.7 cell line) where the methanol extract of A. nilagirica (AN-ME) was found to be safe even at a dose of 500 μg/ml with > 90% cell viability (Fig. 3). But the hydro-alcoholic extract of C. maxima (CM-HA) was found to be toxic at the maximum dose of 500 μg/ml with < 50% cell viability (Fig. 3). After that, the CC50 values of the extracts were determined where the CC50 value of the CM-HA was found to be 817.3 ± 2.4 μg/ml whereas the CC50 value of the AN-ME was found to be > 1000 μg/ml. Overall both the extracts (CM-HA and AN-ME) were found to be more selective towards P. falciparum than normal cells. Besides extracts or compounds with CC50 > 90 μg/ml against normal cell line are considered to be safe and can be taken for further study [46], hence the two test extracts can be utilized for further preclinical studies.

The preliminary phytochemical and fingerprint analysis provide an idea regarding the presence of different plant metabolites in the selected extract [47]. As from the analysis, no known markers were found for both the extracts, the qualitative fingerprints were developed in a controlled environment of temperature and humidity using a suitable mobile phase. The photographs as well the chromatograms developed during the study gave an idea that the CM-HA contained diverse groups of compounds with absorbance and fluorescence characteristics (Fig. 4). The intensities of the bands also indicate the presence of significant amounts of few compounds in the extract. Similarly, the photographs and chromatograms of AN-ME also showed the presence of diverse groups of compounds but the intensity of the bands indicated the presence of those compounds in less quantity (Fig. 5). These two final extracts (CM-HA and AN-ME) were found to be highly active (< 5 μg/ml) with the presence of various important secondary metabolites. These active extracts can deliver new/novel lead compound(s) with antimalarial activity as well as can be further utilized for the development of phytopharmaceutical products by fulfilling the criteria of regulatory body like CDSCO (Central Drugs Standard Control Organisation) for global acceptance.

Conclusion

In this study, we investigated the antimalarial activity of different extracts of two traditionally used medicinal plants C. maxima and A. nilagirica against both chloroquine sensitive (3D7) and chloroquine resistant (RKL-9) strains of P. falciparum. From the results obtained from in vitro experiments, it was observed that hydro-alcoholic extract of C. maxima and methanolic extracts of A. nilagirica showed the potent activity against the strains of P. falciparum without having any toxic effect. The findings of the study will help in identification of active antimalarial lead molecule(s) from the active extracts to develop safe and potent antimalarial drug(s) or phytopharmaceutical(s) by following proper standardization techniques as per the regulatory guidelines.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Abbreviations

A. Nilagirica :

Artemisia nilagirica (C.B. Clarke) Pamp

C. maxima :

Citrus maxima (Burm.) Merr

AN-ME:

Methanol extract of A. nilagirica

CM-HA:

Hydro-alcoholic extract of C. maxima

RPMI-1640:

Roswell Park Memorial Institute-1640

IC50 :

50% inhibitory concentration

PBS:

Phosphate buffer solution

DMEM:

Dulbecco’s Modified Eagle’s medium

FBS:

Fetal Bovine Serum

CC50 :

50% cytotoxic concentration

CDSCO:

Central Drugs Standard Control Organisation

References

  1. Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod. 2016;79(3):629–61. https://doi.org/10.1021/acs.jnatprod.5b01055.

    CAS  Article  PubMed  Google Scholar 

  2. Gurnani N, Mehta D, Gupta M, Mehta BK. Natural products: source of potential drugs. Afr J Basic Appl Sci. 2014;6(6):171–86.

    Google Scholar 

  3. Renslo AR. Antimalarial drug discovery: from quinine to the dream of eradication. ACS Med Chem Lett. 2013;4(12):1126–8. https://doi.org/10.1021/ml4004414.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. Fernández-Álvaro E, Hong WD, Nixon GL, O’Neill PM, Calderón F. Antimalarial chemotherapy: natural product inspired development of preclinical and clinical candidates with diverse mechanisms of action. J Med Chem. 2016;59(12):5587–603. https://doi.org/10.1021/acs.jmedchem.5b01485.

    CAS  Article  PubMed  Google Scholar 

  5. Willcox M, Bodeker G, Rasoanaivo P, Addae-Kyereme J. Malaria control in africa and the role of traditional medicine. In: Willcox M, Bodeker G, Rasoanaivo P, editors. Traditional medicinal plants and malaria. Boca Raton: CRC Press; 2004. p. 1–19.

    Chapter  Google Scholar 

  6. Abirami A, Nagarani G, Siddhuraju P. In vitro antioxidant, anti-diabetic, cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and C. maxima fruits. Food Sci Hum Wellness. 2014;3(1):16–25. https://doi.org/10.1016/j.fshw.2014.02.001.

    Article  Google Scholar 

  7. Barrion ASA, Hurtada WA, Papa IA, Zulayvar TO, Yee MG. Phytochemical composition, antioxidant and antibacterial properties of pummelo (Citrus maxima (Burm.)) Merr. Against Escherichia coli and & Salmonella typhimurium. Food Nutr Sci. 2014;5(9):749–58. https://doi.org/10.4236/fns.2014.59085.

    Article  Google Scholar 

  8. Buachan P, Chularojmontri L, Wattanapitayakul SK. Selected activities of Citrus Maxima merr. Fruits on human endothelial cells: enhancing cell migration and delaying cellular aging. Nutrients. 2014;6(4):1618–34. https://doi.org/10.3390/nu6041618.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. Potdar VH, Kibile SJ. Evaluation of antidepressant-like effect of Citrus Maxima leaves in animal models of depression. Iran J Basic Med Sci. 2011;14(5):478–83.

    PubMed  PubMed Central  Google Scholar 

  10. Chowdhury MRH, Sagor MAT, Tabassum N, Potol MA, Hossain H, Alam MA. Supplementation of Citrus maxima peel powder prevented oxidative stress, fibrosis, and hepatic damage in carbon tetrachloride (CCl4) treated rats. Evid Based Complement Altern Med. 2015;2015:598179.

    Article  Google Scholar 

  11. Kundusen S, Gupta M, Mazumder UK, Haldar PK, Saha P, Bala A. Antitumor activity of Citrus maxima (Burm.) Merr. leaves in ehrlich’s ascites carcinoma cell-treated mice. ISRN Pharmacol. 2011;2011:138737.

    Article  Google Scholar 

  12. Yathiender S. A comparative study of antimicrobial activity of Citrus Maxima and Citrus Aurantium plant extracts. Int J Recent Sci Res. 2017;8(7):18507–9.

    Google Scholar 

  13. Das S, Borah M, Ahmed S. Antibacterial activity of the ethanolic extract of leaves of Citrus maxima (Burm.) Merr. On Escherichia coli and Pseudomonas aeruginosa. Asian J Pharm Clin Res. 2013;6(4):136–9.

    Google Scholar 

  14. Kundusen S, Gupta M, Mazumder UK, Haldar PK, Saha P, Bhattacharya S, et al. Antihyperglycemic effect and antioxidant property of Citrus maxima leaf in streptozotocin induced diabetic rats. Diabetol Croat. 2011;40(4):113–20.

    Google Scholar 

  15. Yetein MH, Houessou LG, Lougbégnon TO, Teka O, Tente B. Ethnobotanical study of medicinal plants used for the treatment of malaria in plateau of Allada, Benin (West Africa). J Ethnopharmacol. 2013;146(1):154–63. https://doi.org/10.1016/j.jep.2012.12.022.

    Article  PubMed  Google Scholar 

  16. Gogoi N, Chetia D, Gogoi B, Das A. Multiple-targets directed screening of flavonoid compounds from citrus species to find out antimalarial lead with predicted mode of action: an in silico and whole cell-based in vitro approach. Curr Comput Aided Drug Des. 2021;17(1):69–82. https://doi.org/10.2174/1573409916666191226103000.

    CAS  Article  PubMed  Google Scholar 

  17. Suseela V, Gopalakrishnan VK, Chandrapraba D. Evaluation of free radical scavenging potential of ethanolic extract of Artemisia nilagirica (Clarke) Pamp. Leaves. Biosci Biotechnol Res Asia. 2009;6(1):189–95.

    CAS  Google Scholar 

  18. Suseela V, Gopalakrishnan VK, Varghese S. In vitro antioxidant studies of fruits of Artemisia nilagirica (Clarke) Pamp. Indian J Pharm Sci. 2010;72(5):644–9. https://doi.org/10.4103/0250-474X.78538.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. Bhat MY, Gul MZ, Khan N, Qureshi IA, Ghazi IA. Cytoprotective activity and anti-inflammatory properties of Artemisia nilagirica (Clarke) extracts- a study with macrophages. J Biol Act Prod from Nat. 2017;7(3):228–44.

    CAS  Google Scholar 

  20. Gul MZ, Chandrasekaran S, Manjulatha K, Bhat MY, Maurya R, Qureshi IA, et al. Bioassay-guided fractionation and in vitro antiproliferative effects of fractions of Artemisia nilagirica on THP-1 cell line. Nutr Cancer. 2016;68(7):1210–24. https://doi.org/10.1080/01635581.2016.1205900.

    Article  PubMed  Google Scholar 

  21. Ahameethunisa AR, Hopper W. Antibacterial activity of Artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria. BMC Complement Altern Med. 2010;10(1):6. https://doi.org/10.1186/1472-6882-10-6.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Gul MZ, Bhat MY, Maurya R, Qureshi IA, Ghazi IA. In vitro evaluation of antioxidant and antiproliferative activities of Artemisia nilagirica extracts. Indian J Pharm Sci. 2017;79(6):872–84.

    CAS  Article  Google Scholar 

  23. Devmurari VP, Jivani NP. Anticancer evaluation of Artemisia nilagirica. Int J Pharm Tech Res. 2010;2(2):1603–8.

    Google Scholar 

  24. Sahu N, Meena S, Shukla V, Chaturvedi P, Kumar B, Datta D, et al. Extraction, fractionation and re-fractionation of Artemisia nilagirica for anticancer activity and HPLC-ESI-QTOF-MS/MS determination. J Ethnopharmacol. 2018;213:72–80. https://doi.org/10.1016/j.jep.2017.10.029.

    CAS  Article  PubMed  Google Scholar 

  25. Naik SK, Mohanty S, Padhi A, Pati R, Sonawane A. Evaluation of antibacterial and cytotoxic activity of Artemisia nilagirica and Murraya koenigii leaf extracts against mycobacteria and macrophages. BMC Complement Altern Med. 2014;14(1):87. https://doi.org/10.1186/1472-6882-14-87.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Panneerselvam C, Murugan K, Kovendan K, Kumar PM. Mosquito larvicidal, pupicidal, adulticidal, and repellent activity of Artemisia nilagirica (family: Compositae) against Anopheles stephensi and Aedes aegypti. Parasitol Res. 2012;111(6):2241–51. https://doi.org/10.1007/s00436-012-3073-9.

    Article  PubMed  Google Scholar 

  27. Panda S, Ranjit MR, Sahoo SL, Pati P, Sahoo R. Larvicidal, pupicidal, adulticidal and repellent activity of Artemisia nilagirica (C.B. Clarke) Pamp against malarial vector Anopheles stephensi. Adv Life Sci. 2016;5(511):4424–8.

    Google Scholar 

  28. Verma RP, Subburaju T, Balakrishnan N. Larvicidal activity of Artemisia nilagirica (Clarke) Pamp. And Ocimum sanctum Linn. A preliminary study. J Nat Remedies. 2006;6(2):157–61.

    Google Scholar 

  29. Panda S, Rout JR, Pati P, Ranjit M, Sahoo SL. Antimalarial activity of Artemisia nilagirica against Plasmodium falciparum. J Parasit Dis. 2018;42(1):22–7. https://doi.org/10.1007/s12639-017-0956-9.

    Article  PubMed  Google Scholar 

  30. WHO guidelines on good agricultural and collection practices (GACP) for medicinal plants. World Health Organization. 2003. https://www.who.int/medicines/publications/traditional/gacp2004/en/. Accessed 1 Dec 2020.

  31. Singh J. Maceration, percolation and infusion techniques for the extraction of medicinal and aromatic plants. In: Handa SS, Khanuja SPS, Longo G, Rakesh DD, editors. Extraction technologies for medicinal and aromatic plants. Trieste: ICS-UNIDO; 2008. p. 67–82.

    Google Scholar 

  32. Trager W, Jensen JB. Human malaria parasites in continuous culture. Science. 1976;193(4254):673–5. https://doi.org/10.1126/science.781840.

    CAS  Article  PubMed  Google Scholar 

  33. Kumawat MK, Chetia D. Synthesis, antimalarial activity evaluation and molecular docking studies of some novel dispiro-1,2,4,5-tetraoxanes. Bangladesh J Pharmacol. 2015;10(4):917–23. https://doi.org/10.3329/bjp.v10i4.24532.

    Article  Google Scholar 

  34. Lambros C, Vanderberg JP. Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol. 1979;65(3):418–20. https://doi.org/10.2307/3280287.

    CAS  Article  PubMed  Google Scholar 

  35. Pandey AK, Sharma S, Pandey M, Alam MM, Shaquiquzzaman M, Akhter M. 4, 5-Dihydrooxazole-pyrazoline hybrids: synthesis and their evaluation as potential antimalarial agents. Eur J Med Chem. 2016;123:476–86. https://doi.org/10.1016/j.ejmech.2016.07.055.

    CAS  Article  PubMed  Google Scholar 

  36. Lima RBS, Silva LFR, Melo MRS, Costa JS, Picanço NS, Lima ES, et al. In vitro and in vivo anti-malarial activity of plants from the Brazilian Amazon. Malar J. 2015;14(1):508. https://doi.org/10.1186/s12936-015-0999-2.

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  37. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1–2):55–63. https://doi.org/10.1016/0022-1759(83)90303-4.

    CAS  Article  PubMed  Google Scholar 

  38. Seca AML, Pinto DCGA. Biological potential and medical use of secondary metabolites. Medicines. 2019;6(2):66. https://doi.org/10.3390/medicines6020066.

    CAS  Article  PubMed Central  Google Scholar 

  39. Mahire SP, Patel SN. Extraction of phytochemicals and study of its antimicrobial and antioxidant activity of Helicteres isora L. Clin Phytoscience. 2020;6(1):40. https://doi.org/10.1186/s40816-020-00156-1.

    CAS  Article  Google Scholar 

  40. Ram M, Abdin MZ, Khan MA, Jha P. HPTLC fingerprint analysis: a quality control for authentication of herbal phytochemicals. In: Srivastava M, editor. High-performance thin-layer chromatography. Berlin: Springer; 2011. p. 105–16. https://doi.org/10.1007/978-3-642-14025-9_7.

    Chapter  Google Scholar 

  41. Itankar P, Sawant D, Tauqeer M, Charde S. High performance thin layer chromatography fingerprinting, phytochemical and physico-chemical studies of anti-diabetic herbal extracts. AYU. 2015;36(2):188–95. https://doi.org/10.4103/0974-8520.175546.

    Article  PubMed  PubMed Central  Google Scholar 

  42. Goswami AK, Gogoi N, Shakya A, Sharma HK. Development and validation of high-performance thin-layer chromatographic method for quantification of berberine in rhizomes of Coptis teeta wall, an endangered species collected from Arunachal Pradesh. India J Chromatogr Sci. 2019;57(5):411–7. https://doi.org/10.1093/chromsci/bmz009.

    CAS  Article  PubMed  Google Scholar 

  43. Tse EG, Korsik M, Todd MH. The past, present and future of anti-malarial medicines. Malar J. 2019;18(1):93. https://doi.org/10.1186/s12936-019-2724-z.

    Article  PubMed  PubMed Central  Google Scholar 

  44. Pan WH, Xu XY, Shi N, Tsang SW, Zhang HJ. Antimalarial activity of plant metabolites. Int J Mol Sci. 2018;19(5):1382. https://doi.org/10.3390/ijms19051382.

    CAS  Article  PubMed Central  Google Scholar 

  45. Ouattara LP, Sanon S, Mahiou-Leddet V, Gansané A, Baghdikian B, Traoré ASA, et al. In vitro antiplasmodial activity of some medicinal plants of Burkina Faso. Parasitol Res. 2014;113(1):405–16. https://doi.org/10.1007/s00436-013-3669-8.

    Article  PubMed  Google Scholar 

  46. Cytotoxicity: in vitro determination. World Health Organization. https://www.who.int/tdr/grants/workplans/en/cytotoxicity_invitro.pdf. Accessed 5 Dec 2020.

  47. Panda SK, Das R, Leyssen P, Neyts J, Luyten W. Assessing medicinal plants traditionally used in the Chirang reserve forest, Northeast India for antimicrobial activity. J Ethnopharmacol. 2018;225:220–33. https://doi.org/10.1016/j.jep.2018.07.011.

    CAS  Article  PubMed  Google Scholar 

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Acknowledgements

The authors are thankful to the Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India for providing the laboratory facilities to conduct the research work. The Authors also are thankful to the Parasite Bank of National Institute of Malaria Research (Indian Council of Medical Research), New Delhi for providing the malaria parasite strains to carry out the research work.

Funding

The work was supported by University Grants Commission, New Delhi, India under UGC-SAP (DRS-I) [F.3–13/2016/DRS-I (SAP-II)].

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Faculty of Science and Engineering, Dibrugarh University, Dibrugarh, Assam, 786004, India

    Neelutpal Gogoi & Dipak Chetia

  2. Department of Biotechnology, Royal School of Bio-Science, Royal Global University, Guwahati, India

    Bhaskarjyoti Gogoi

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Contributions

NG and DC conceived and designed the experiments; NG & BG performed the in vitro experiments and analysed the results; NG wrote the manuscript, and BG & DC reviewed the manuscript. All authors read and approved the final manuscript.

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Correspondence to Neelutpal Gogoi.

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Gogoi, N., Gogoi, B. & Chetia, D. In vitro antimalarial activity evaluation of two ethnomedicinal plants against chloroquine sensitive and resistant strains of Plasmodium falciparum. Clin Phytosci 7, 42 (2021). https://doi.org/10.1186/s40816-021-00269-1

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Keywords

  • Malaria
  • Plasmodium falciparum
  • Citrus maxima (Burm.) Merr.
  • Artemisia nilagirica (C.B. Clarke) Pamp.
  • Cytotoxicity study, herbal remedy