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Neuromodulatory effect of solvent fractions of Africa eggplant (Solanium dadyphyllum) against KCN-induced mitochondria damage, viz. NADH-succinate dehydrogenase, NADH- cytochrome c reductase, and succinate-cytochrome c reductase
Clinical Phytoscience volume 4, Article number: 9 (2018)
In the past few years, there has been a tremendous increase in the number of plant-based health supplements with respect to their safety and efficacy in diseases treatment and prevention. Solanum dasyphyllum, also known as Africa eggplant is ethnomedicinally used as an antivenom, pain reliever and anticonvulsant in various part of Nigeria, however, there is no scientific data to support some of these claims.
This study evaluated the protective effect of solvent fractions of Solanum dasyphyllum, hexane fraction of S. dasyphyllum (HFSD), dichloromethane fraction of S. dasyphyllum (DFSD), ethylacetate fraction of S. dasyphyllum (EAFSD), methanolic fraction of S. dasyphyllum (MFSD) and crude fraction of S. dasyphyllum (CFSD) on cyanide-induced oxidative stress and neurotoxicity in vitro in the cerebral cortex. Neuroprotective activities were evaluated by assaying for markers of oxidative stress, neurotoxicity and electron transport system enzymes via evaluating lipid peroxidation (LPO), protein carbonyl (PC), reduced glutathione (GSH), acetylcholinesterase (AChE), NADH-succinate dehydrogenase (NSD), NADH-cytochrome c reductase (NCR), and succinate-cytochrome c reductase (SCR) in the homogenate of cerebral cortex.
The results showed that all solvent fractions of S. dasyphyllum significantly ameliorated cyanide-induced oxidative stress (P < 0.05). It inhibited the activity of acetylcholinesterase-HFSD (68.42 ± 5.37%), DFSD (36.32 ± 5.45%), EFA (20 ± 0.69%), MFSD (33.16 ± 4.8%) and CFSD (35.79 ± 2.8%), increased the activity of NSD [HFSD (94.74 ± 7.3%), EAFSD (78.95 ± 5.4%) and CFSD (60.53 ± 4.6%)], while DFSD (− 5.26 ± 1.4%) and MFSD (− 7.9 ± 0.4%) had a negative effect, increased the activity of NCR [HFSD (91.89 ± 7.1%), DFSD (90.54 ± 8.2%), EAFSD (62.16 ± 4.7%); MFSD (306.76 ± 7.2%) and CFSD (154.0 ± 8.1%)]. All the solvent fractions also significantly increased the activity of SCR [HFSD (70.59 ± 3.8%), EAFSD (58.82 ± 6.4%), MFSD (88.24 ± 9.0%) CFSD (76.47 ± 8.2%)] apart from DFSD (− 5.88 ± 1.2%) in rat cerebral cortex.
This result shows that S. dasyphyllum has neuroprotective activities, however HFSD shows the most potent bioactivities in maintaining mitochondria integrity by preserving the electron transport system. Further work can be done on isolating and characterizing the bioactive compound in HFSD for novel natural product in the treatment of neurological disorders.
The brain is one of the complex and delicate organ in the body, such that some of the cells survive the entire lifespan of human . The brain is a key component of the nervous system, thus, it affects various neuromuscular activities in the body. The aging of brain cells causes an increase in reactive oxygen species generation, coupled with low level of antioxidant system in the brain leads to oxidative stress (OS) . Thus several normal physiological functions such as memory and voluntary movement controlled sby cortex are reduced or lost, while postural stability, movement coordination coordinated by the cerebellum are also lost. Deficient or loss of this function is termed neurodegenerative diseases . With increase in aged population, it is estimated that by 2050, the number of patient suffering from neurodegenerative diseases will double .
Mitochondria are described as “the powerhouse of the cell” because they generate most of the cell’s supply of adenosine triphosphate (ATP), used as a source of chemical energy. In addition to supplying cellular energy, mitochondria are involved in other tasks, such as signallingcellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiac dysfunction, heart failure and neurological disorders [4, 5].
Cyanide is often found joined with other chemicals to form compounds. Examples of simple cyanide compounds are hydrogen cyanide sodium cyanide and potassium cyanide and hydrogen cyanide is the main form present in the air. Cyanide can be released into the environment during the course of industrial usage, from smoke or vehicles exhaust containing the incomplete combustion products of nitrogen-containing organic polymers. Also, several plants contain cyanogen glycosides that can release hydrogen cyanide upon biodegradation or digestion .
Cyanides are highly toxic due to their binding to the ferric form of vital cellular enzymes such as cytochrome oxidase, succinic dehydrogenase and superoxide dismutase, with particular interest on the electron transport system in the mitochondria . The binding of cyanide to this mitochondrial enzyme prevent the transport of electron from cytochrome c to oxygen resulting in disruption of the electron transport chain and inability of cells to aerobically produce ATP. This leads to hypoxia and tissue damage, with the most vulnerable tissues being those with high oxygen demands such as brain and heart [8, 9]. Other toxic effect of cyanide includes binding to affecting neurotransmitter receptor N-methyl-daspartate (NMDA) receptor to increase the release of glutamate and elevate the activity of acetylcholinesterase, an enzyme that catalyse the degradation of acetylcholine and also increase the generation of reactive oxygen species . Although antidotes to cyanide poisoning exist, these have limitations and side effects . Therefore, search for highly effective and safe agents across different scenarios of cyanide toxicity is essential.
Some Herbal products that are perceived to be more effective and have fewer side effects in therapeutics are gaining increasing prominence than synthetically derived drugs in healthcare . Solanum dasyphyllum, otherwise known as the African eggplant or gboma leaves has been used locally as antidotes (venomous stings, bites etc),treatment of tooth aches, stomach pains, parasitic infections, swellings etc. It is known to have anticonvulsant, neuromodulatory and cardiovascular (hypotensive) properties.
The present study was designed to ascertain the potential of S. dasyphyllum, a locally used anticonvulsant plant, to protect against mitochondria damage and neurotoxicity induced by KCN .
Chemicals and reagents
Thiobarbituric acid (TBA), trichloroacetic acid (TCA), ubiquinone, perchloric acid (PCA), benzylamine hydrochloride (BAHC), sodium dodecyl sulphate (SDS), ethylene diamine tetraacetic acid (EDTA), acetylcholine iodide, 2,4-dinitrophenyl hydrazine, cytochrome and reduced nicotinamide adenine dinucleotide phosphate (NADH) were obtained from Sigma-Aldrich (St-Louis, MO, USA). Randox Kits were used for total protein assay purchased from Randox laboratory lid, United Kingdom. All other chemicals and reagents were of analytical grade.
Plant collection and preparation of extract
Male Wister rats with weighing 200 g were bred and housed at the animal house of the Department of Animal Production and Health, Federal University of Technology, Akure, Nigeria. Animals were kept under standard laboratory condition and fed with laboratory chow (vital feed lagos, Nigeria) and water ad libitum. All animals’ experimental protocols conformed to the guidelines of National Institute of Health for laboratory animals.
Identification and collection of plant materials.
African Egg Plant (Solanum dasyphyllum) belonging to the family Solanaceae. Leaves of the plant was collected, identified at obafemi awolowo university ile-ife, with the voucher number IFE-17489 and the leaves were air dried in the laboratory (25–28 °C) and grounded into powder form with the aid of an electric blender.
The powdered forms of the leaf were soaked with absolute methanol for 72 h with constant stirring and was filtered using Whatman No1 filter paper, and the filtrate was concentrated using a rotary evaporator, followed by lyophilizing to dryness.
The crude extract of S. dasyphyllum was subjected to solvent partitioning using n-hexane, dichloromethane ethylacetate and methanol (according to their polarity). 15 g of the crude extract was weighed, dissolved in 100 ml of warm water and poured into inclined separating funnel. To obtain hexane fraction of S. dasyphyllum (HFSD), 600mls of n-hexane was added into the separating funnel, shake gently and allowed to separate on standing. The residue and the supernatant fractions were collected. The residue was returned back into the separating funnel and 800mls of n-hexane was added, shaken, and allowed to separate on standing. The same procedure as above was followed for the collection of dichloromethane (DCM) fraction of S. dasyphyllum (DFSD) and 800mls of DCM was used, ethylacetate (EA) fraction of S. dasyphyllum (EAFSD), 700mls of EA was used and methanolic fraction of S. dasyphyllum (MFSD) 1000mls of methanol was used. The fractions (n- hexane, dichloromethane, ethylacetate and methanol) were concentrated to dryness using rotary evaporator and weighed.
Cerebellum and cortex preparation
Cortex and cerebellum preparation was done by differential centrifugation method . The rats were sacrificed by cervical dislocation without anaesthesia and the brain was rapidly excised on the petri dish placed on ice, the blood and eternal vessels was carefully removed. The cerebellum and the cerebral cortex regions of the brain were carefully dissected, weighed and separately homogenized in 0.1 M phosphate buffer at pH 7.4 with a manual glass-teflon homogenizer on ice to obtain 10% w/v homogenate and centrifuged at 6000 rpm at 4 °C for 25 min to obtain the supernatant, stored in an amber bottle and refrigerated, which was used for biochemical analyses.
Evaluation of the neuroprotective effect of S. dasyphyllum in rat brain homogenate challenged with cyanide
To investigate the neuroprotective activities of S. dasyphyllum, the cerebellum and cortex homogenate mixture were incubated with 0.09 mM of potassium cyanide (KCN), in the presence or absence of varying concentrations of crude extracts and fractions (HFSD, DFSD, EAFSD and MFSD) of S. dasyphyllum leaves at 37 °C for 1 h respectively was used to induce mitochondria damage under in vitro condition.
Group I: Control; contained homogenate and distilled water.
Group II: KCN; contained homogenate and toxicant.
Group III: 5 μg/ml + KCN; contained homogenate and (5 μg) extract and toxicant.
Group IV: 10 μg/ml + KCN; contained homogenate and (10 μg) extract and toxicant.
Group V: 20 μg/ml + KCN; contained homogenate and (20 μg) extract and toxicant.
Group VI: 30 μg/ml + KCN; contained homogenate and (30 μg) extract and toxicant.
Group VII: 40 μg/ml + KCN; contained homogenate and (40 μg) extract and toxicant.
Group VIII: 50 μg/ml + KCN; contained homogenate and (50 μg) extract and toxicant.
Determination of reduced glutathione (GSH) concentration
Estimation of GSH was determined according to the method of . Sample (100 μl) was added to 900 μl of distilled water and 1500 μl of the precipitating solution (Sulphosalicyclic acid) was mixed with the sample. The rate of addition was not critical. A blank was prepared by replacing the volume of sample with distilled water i.e. a total of 1000 μl of distilled water. The mixture was then allowed to stand for approximately 5 min and then filtered. At the end of the fifth minute, 500 μl of filtrate was added to 250 μl of 0.1 M phosphate buffer. Finally 2250 μl of the Ellman’s reagent was added. The optical density was measured at 412 nm and the amount of reduced GSH was determined using an extinction coefficient of 9600 M− 1CM− 1.
Evaluation of lipid peroxidation (LPO)
The LPO inhibitory activity was evaluated by measuring the formation of TBA reactive substances (TBARS) according to the method of . An aliquot of 0.4 ml of the sample was mixed with 1.6 ml of TRIS-KCl buffer to which 0.5 ml of 30% TCA was added. Then 0.5 ml of 0.75% TBA was added and placed in a water bath for 45 min at 80 C. This was then cooled in ice and centrifuged at 3000 g. The clear supernatant was collected and absorbance measured against a reference blank of distilled water at 532 nm. The TBARS level was calculated according to the method of . LPO in units/mg protein was computed with a molar extinction coefficient of 1.56 × 105 M-.
Determination of protein carbonyl (PC) content
PC content in the brain regions was determined according to the method of . Brain supernatant was incubated with DNPH for 60 min at room temperature. Following precipitation by adding 20% TCA, the pellet was washed with acetone and dissolved in 1 ml of TRIS buffer containing sodium dodecyl sulphate (8% w/v, pH 7.4). The absorbance was measured at 360 nm and expressed as nmol carbonyls/mg protein.
Evaluation of NADH – Cytochrome c and succinate –cytochrome c reductase activity 
The activities of NADH–cytochrome C reductase (NCR) and succinate–cytochrome C reductase (SCR) were measured in brain mitochondria as described previously . Briefly mitochondrial protein (0.1 mg) was added to phosphate buffer (0.1 M, pH 7.4) containing NADH (0.2 mM) and KCN (1 mM). The reaction was initiated by the addition of 0.1 mM cytochrome C giving a final volume of 1 ml reaction mixture and decrease in absorbance was monitored for 3 min at 550 nm. The activity was expressed as nmol cytochrome C reduced/min/mg protein 273 (€-19.6/mM/cm). To determine the activity of SCR, the substrate succinate (20 mM) was used.
Evaluation of NADH – Succinate dehydrogenase (NSD) activity 
Briefly, 0.05 ml of the reaction mixture was incubated with 0.8 ml phosphate buffer (50 mM), 0.1 ml sodium succinate (0.01 M) and 0.025 ml p-iodonitro tetrazolium (INT) (2.5 μg/ml) at room temperature for 10 min. After incubation, 0.5 ml TCA (10%) and 0.5 ml ethylacetate/ethanol/TCA (5:5:1, v: v: w) was added. Absorbance was read at 490 nm. Results expressed as nmol of INT/ mg protein.
Evaluation of Acetylcholineterase (AChE) activity
AChE activity was evaluated according to . DTNB (0.01 M, 0.1 ml) was added to 2.6 ml of 0.1 M phosphate buffer (pH 8.0). Sample (0.04 ml) was added to the mixture followed by incubation for 5 min. Then, 0.04 ml of substrate (0.075 M acetylthiocholine iodide) was added to the reaction mixture. The readings were taken at 420 nm continuously for 3 min at 30 s intervals. AChE activity was calculated using a molar extinction coefficient 1.36 × 10 − 4 M − 1 cm − 1 and expressed in μmol− 1 min − 1 mg protein− 1.
Evaluation of Total protein concentration
Total protein level This was determined using an assay kit from Randox Laboratory Ltd., Antrim, UK following the instructions of the manufacturer.
Results were expressed as mean ± standard deviation (SD). Statistical differences between means were determined by oneway analysis of variance (ANOVA) followed by Duncan’s test. P < 0.05 was considered statistically significant.
Table 1 reveals that the level of AchE was significantly higher in KCN intoxicated untreated group when compared with control group (P < 0.05), while in the Solanum dasyphyllum treated group, the activity significantly decreased as compared to the induced group (P < 0.05) in a non concentration-dependent manner. It can also be observed from the results that EAFSD at lower concentrations possess a better anticholinesterase activity as compared to other fractions.
Tables 2, 3 and 4 reveals the protective effect of S. dasphyllum against cyanide inhibitory action against the activity of electron transport chain enzymes (NCR, SCR and) in the cerebrocrotex. The figures showed that the protective activity of each fractions varies with the enzyme assayed for. While DFSD was generally protective against the inhibitory effect of KCN on NCR. MFSD was protective in all except NSD where there was no significant difference between the enzyme activity in the fraction and KCN group. HFSD, EFSD and CFSD significantly reverse the inhibitory activity of KCN in all the ETC enzymes evaluated.
Figures 1, 2 and 3 showed the antioxidant effect of all the fractions against KCN induced toxicity in the cerebrocortex. The crude and hexane fractions showed a significant increase in the concentration of GSH as compared to the KCN group, while there was no significant difference between the cyanide group and all other fractions (P < 0.05). However, all the fractions significantly prevent PC formation and LPO in the cerebrocortex as compared to the control (P < 0.05).
Findings in the results showed that S. dasyphyllum reverse the neurotoxicity of KSN by preserving mitochondria integrity through the preservation of electron transport chain enzymes. The ease of accessibility and lower toxicity of phytochemicals has rekindled the global research on medicinal plants as a drug of choice in the treatment of diseases. The neuromuscular and hypotensive properties of phytochemicals isolated from S. dasyphylum has also been reported , this includes, coumarin and scopoletin . Neurodegenerative disease such as Alzheimer’s disease and Parkinson’s disease has been linked to mitochondria dysfunction . Cyanide is also known to be an inhibitor of complex IV in the electron transport chain, leading to severe depletion of ATP and cell death that is dependent on energy impairment). KCN has been reported to alter the metabolism of acetylcholine via increasing the activity of AChE . The current data corroborated the increased activity of AChE in the cerebrocortex by KCN. KCN has been known to stimulate hypoxia cell death via the reduction in the availability of acetylcholine to the synaptic cells . All the solvent fractions significantly reverse the increased acetylcholinesterase activity induced by KCN. EAFSD was not effective at low concentration as compared to other solvent fractions, however, at higher concentration, it significantly reversed the activity of AChE. One of the key targets in the development of drugs for the treatment of neurological disorders is the search for AChE inhibitors . The AChE inhibitory activity of all the solvent fraction of S. dasyphyllum might be as a result of various phytochemicals present in the plants, such as alkaloids, flavonoids, coumarin, and steroids (unpublished), that have been reported to possess anticholinesterase activity . Thus, S. dasyphyllum can be classified among anticholinesterase plants in the treatment of AChE-related neurological disorders.
The activity of ETC is central to the integrity of mitochondria, in the present study, KCN being a high-affinity ETC blocker significantly disrupted the enzyme activities across the mitochondria membrane in the cerebrocortex, with reduced activity of enzymes involved in electron transport (NADH-cytochrome c reductase [NCR], succinate-cytochrome c reductase [SCR] and NADH-succinate dehydrogenase [NSD] as compared to the control in the cerebrocortex. The sensitivity of the enzymes to KCN was clearly observed in the experiment, with NCR more sensitive to KCN than SCR. This inhibition can lead to energy deficit and electron leakage, further increasing free radical generation [24,25,26]. All the solvent fractions of S. dasyphyllum were able to reverse the inhibitory effect of KCN on ETC enzymes. One of the central point in the mechanism of disease prevention by phytochemicals is through the protection of mitochondria against injury [27,28,29]. Such phytochemicals are found in plants like baicalein, Parkia biglobosa, Antiaris africana and curcumin [30,31,32,33]. KCN significantly elevated PC and LPO generation in the cerebrocortex. This might be as a result of the inhibitory activity of KCN on the ETC, leading to increased generation of ROS, that reacts with functional protein and lipids, causing loss of function. This is not different from the report of . The low level of LPO and PC observed in the cerebrocortex upon co-treatment with different solvent fractions of S. dasyphyllum suggesting a possible antioxidative role of the fractions in slowing oxidative stress process and accumulation of their products in the brain, which may be one of the mechanisms by which the plants offer neuroprotection. One of the central activities of phytochemicals is in the mopping up of ROS, termination of chain reactions or donation of electrons to stop their reaction with macromolecules. There is no doubt that these antioxidant activities exemplified by various fractions of S. dasyphyllum play a significant role in the medicinal usage of the plant in the treatment of diseases. GSH is the major antioxidant molecule in the brain, which protects the brain against diverse pathogens , decreased GSH level is a distinguishing feature of KCN-exposed glial cells, and that deficiency of glutathione leads to pathological conditions in the brain . In general, all the fractions were protective against KCN neurotoxicity, however, the extracts exhibited varying degrees of potency in some of the biochemical assays.
The outcome of this research suggested that all the solvent fractions of S. dasyphylum possess anticholinesterase activity, with mild antioxidant properties. Also observed is the preservation of mitochondria functions in the brain through maintenance of the activities of electron transport chain. These properties can be a reason for its usage as a panacea for snake poison and anticonvulsant. Further work can be done to identify and quantify the various bioactive compounds present in each solvent fraction and subject them to further neuroprotective studies.
Hexane Fraction S. dasyphyllum.
Dichloromethane Fraction S. dasyphyllum
Ethylacetate Fraction S. dasyphyllu.
Methanolic Fraction S. dasyphyllum
TBA reactive substances
NADH-cytochrome c reductase
succinate cytochrome c reductase
Reactive Oxygen Species
Electron Transport Chain
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Obade, E., Ilesanmi, O.B., Crown, O. et al. Neuromodulatory effect of solvent fractions of Africa eggplant (Solanium dadyphyllum) against KCN-induced mitochondria damage, viz. NADH-succinate dehydrogenase, NADH- cytochrome c reductase, and succinate-cytochrome c reductase. Clin Phytosci 4, 9 (2018). https://doi.org/10.1186/s40816-018-0068-9
- Electron transport enzymes
- Alternative medicine
- Solanum dasyphyllum
- Oxidative stress
- Neurological disorders