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International Journal of Phytomedicine and Phytotherapy

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African natural products with potential antioxidants and hepatoprotectives properties: a review


Free radicals are highly reactive molecules generated during oxidation reactions which in turn initiate chain reactions resulting to cellular damage. There is substantial evidence implicating free radicals especially reactive oxygen species (ROS) in the etiology of more than one hundred degenerative disorders in humans including, arthritis, atherosclerosis, ischemia and reperfusion injury of many tissues, gastritis, diabetics, central nervous system injury, acquired immunodeficiency syndrome (AIDS) and cancer. Scientific evidence postulates that bioactive compounds especially from natural products are capable of providing protection against free radicals. Consequently, few decades have witnessed a surfeit of research geared towards validating the antioxidant and hepatoprotective potential of the natural products. In this review, African natural products whose antioxidants activities were scientifically validated either in their crude extracts and/or derived products have been discussed. A total 1076 plants species representing 287 family, 132 isolated compounds and 7 insect/mollusk secreation were found. The plant species from the following families; Fababceae, Asteraceae, Lamiaceae, Moraceae, Euphorbiaceae, Combretaceae and Malvaceae have received more scientific attention than others. Analysis of the reports revealed that Combretum apiculatum, Telfaria occidentalis, Acalypha racemosa, Garcinia lucida were the most active plant extracts from African flora. The most active ROS-detoxifying phytochemicals were moracin T, U, S and R (8487), oleanolic acid (54), 5,7,4′–trihydroxy–3,8,3′,5′–tetramethoxyflavone (89), 5,7,3′–trihydroxy-3,8,4′,5′–trimethoxyflavone (88), luteolin (3′,4′,5,7–tetrahydroxy flavone) (117) and genistein (4′,5,7–trihydroxyisoflavone) (116). The significant antioxidant potential demonstrated by some crude extracts and their constituent compounds render them good candidates for the development of new drugs. Although, the study of the mechanisms of actions as well as clinical validation of some of these isolated compounds is lacking. It is hoped that pertinent scientist and stakeholders will look further into some of these compounds for detailed authentification and subsequent commercialization.


Oxidation is a chemical reaction that transfers electrons or hydrogen from a substance to an oxidizing agent. Free radicals are generated during this oxidation reaction especially during oxidative respiration when there is a mitochondria leakage of activated oxygen [1], which in turn initiate a chain of reactions that results in cellular damage. Antioxidants terminate this chain of reactions by removing free radical intermediates, thus inhibiting further oxidation reactions [2]. They include reducing agents such as β-carotene, vitamin C, E and ascorbic acid, as well as enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione and peroxidases [3], and therefore exert their protective role by being oxidized themselves. Furthermore, many antioxidants compounds have been characterized form plants including flavonoids. Flavonoids are phenolic compounds with importants roles in scavenging free radicals and thus play vital roles in preventing oxidative stress associated disorders [4]. Among the common ROS are superoxide (O2·−), hydroxyl (OH), and peroxyl (OOH, ROO) radicals [5]. Enzymes capable of producing superoxide are xanthine oxidase, reduced nicotinamide adenine dinucleotide phosphate oxidases and cytochrome P450 [1]. The imbalance between the production of these free radicals and the detoxifying capacity of the antioxidants results in oxidative stress which is among the major implicative factors in etiology of certain degenerative and chronic diseases including diabetes, atherosclerosis, parkinson’s disease [6], renal disorders [7], cardiovascular, inflammatory, cancer, autoimmune, neurodegenerative diseases [8], and several other human ailments [9].

The liver is the major regulatory organ responsible for the metabolism, storage, detoxification, secretions and excretions of various exogenous and endogenous molecules including xenobiotics [10]. It plays a vital role in maintaining cellular homeostasis and protects the body against deleterious effect of chemicals, drugs, toxin, organism and parasite [5]. Therefore, the healthy performance of the organ reflects the health status of human [11, 12]. However, during these protective roles this organ is susceptible to a numbers of diseases and disorders [13], from chemical drugs and other agents due to its distinctive metabolic roles and the proximal affiliation with the gastrointestinal tract (GIT) [14]. Hepatic injury may also results from excessive alcohol and paracetamol consumptions, exposure to infectious agents, xenobiotics and over the-counter drugs in western countries [15].

Hepatic diseases are a worldwide predicament often involving free radicals induced oxidative stress which if left untreated may advance from steatosis to chronic hepatitis, fibrosis and hepatocellular carcinoma [16]. The conventional drugs commonly used to combat the diseases and disorders associated with the liver are beset with different undesirable effects on biological systems [17]. As a result considerable attentions has been geared towards finding alternative, less toxic and effective antioxidants and hepatocurative agents from Africa natural product for the prevention, managements and treatment of diseases and disorders associated with the liver [18]. The natural products with medicinal reputation could serve as lead sources of natural antioxidants for development of novel drugs [12].

Africa is blessed with enormous biodiversity of natural product for healing practices [19]. From time immemorial Africa medicinal plants have been used by virtually all cultures to meet their health care needs. Evolutions have made plants to harbor a numbers of antioxidant chemicals (phytochemical or secondary metabolites) as natural means of surviving in hostile environments [20]. Consequently, few decades have witnessed a glut of research geared towards validating the quality, quantity, protective roles as well as therapeutic effectiveness of these antioxidant in African plants against oxidative stress induced diseases and disorders.

However, available reviews on the antioxidant potencies of African natural products; focused only on medicinal plants [21], published decade ago with emphasis only on 38 plants [22], others are limited to Cameroonian medicinal plants, [23], few African vegetables, fruits and mushrooms [24], and hepatoprotective activities of medicinal plants [25]. This review is intended to serve as scientific baseline information for the documented African natural products with antioxidants and hepatoprotectve reputation as well as a starting point for future studies.

Methodology (Search strategy)

To identify natural products from African flora and fauna with antioxidant and hepatoprotective potentials, a review was compiled based on scientific literature from various sources including; Google Scholar, Science Direct, PubMed, Medline, Science domain [19, 22, 26, 27]. The keywords used for identification of relevant data included the following terms; antioxidant, radical scavenging activities, anti-aging principles, reactive oxygen species, free radicals, African medicinal plants, natural product, 2,2-Diphenyl-1–picrylhydrazyl radical scavenging assay (DPPH), reducing properties and lipid peroxidations. All relevant data previously published in English were retrieved. However, data for natural products from sources other than African countries were completely excluded from this review paper. Using the specified procedure for acquisition of necessary data, 641 articles were retrieved, out of which 315, mainly in the form of journal articles, books and reviews; were used for compilation of the current review.

The information obtained from these research articles, captured in the current review paper includes; scientific names, that is the family, genus and specific names, parts of plants or mollusk used, solvent system used for the extraction procedure, the bioassay test carried out, whether in vitro or in vivo, as well as the antioxidant and hepatoprotective potencies of natural products originating from African flora and fauna (Tables 1, 2, 3, 4, 5 and 6). Information was also obtained from authenticated post graduate theses, conference proceedings with literature on antioxidant and hepatoprotective assay results of flora and fauna endemic or naturalized in Africa.

Table 1 Antioxidants activities of West African plants
Table 2 Antioxidants activities of Northern Africa African plants
Table 3 Antioxidants activities of Southern AfricaAfrican plants
Table 4 Antioxidants activities of Central African plants
Table 5 Antioxidants activities of Eastern African plants
Table 6 Isolated Compounds from African medicinal plants with antioxidants potential

Results and discussion

A total 1076 plants species representing 287 family and 7 other natural products were identified. Previous phytochemical studies of ethnomedicinal plants of African origin used as antioxidants and for hepatoprotective properties led to characterization of approximately 132 compounds reviewed in this study. A map of Africa indicating the subregions of the continent as used in this review is presented in Fig. 1. From the reviewed plants with antioxidant and related data; 31.33% originate from Northern Africa, 30.97% from Western Africa, 17.98% from Central Africa, 13.98% from Southern Africa, and 5.72% from Eastern Africa (Fig 2). Tables 1, 2, 3, 4, 5 and 6 gives a summary of the plant species that were tested, the family these plants belong to, the parts of the plants that were used to prepare the test samples, the solvent used for the extraction procedure and their potencies in different units depending on the protocol used. The plants that have been extensively studied with regard to these activities belonged to the following families; Fababceae (6.34%), Asteraceae (6.34%), Lamiaceae (5.13%), Moraceae (4.30%), Euphorbiaceae (2.41%), Combretaceae (2.19%), and Malvaceae (1.81%) (Fig. 3). The structures of the compounds isolated from some of the plants with antioxidant activities are presented in (Fig. 4, Additional file 1). The plant parts that were tested for activities included the leaves, stems and stem bark, roots and root bark, pods, flowers and other aerial parts.

Fig. 1

Map of Africa showing the different subregions

Fig. 2

Regional distribution of of investigated African plants with antioxidant potentials

Fig. 3

Percentage occurrence of the most investigated African plants families for antioxidants activities

Fig. 4

Structure of chemical compounds isolated from African plants with potential antioxidants and hepatoprotectives properties (Additional file 1)

A number of procedures have been developed for assessment of in vitro antioxidant potencies of natural products. These protocols are based on two major chemical reactions including; hydrogen atom and electron transfer reactions. To determine the antioxidant potencies of the extracts and compounds using the hydrogen atom transfer mechanisms, one of the following parameters are measured; oxygen radical absorbance capacity (ORAC), total radical antioxidant power (TRAP) and beta carotene bleaching potential. The second category involves electron transfer reactions that measures the following parameters; ferric reducing antioxidant power (FRAP), diphenyl-2–picryl-hydrazyl radical scavenging assay (DPPH), trolox equivalent antioxidant capacity (TEAC), hydroxyl radical scavenging assay, superoxide anion radical scavenging assay, nitric oxide radical scavenging assay and total phenol assay [28]. Despite the recent popularity in antioxidant research, lack of standardized assays to compare research results from different research groups has been a major challenge [29].

The antioxidant potencies of natural products reviewed in this study were categorized based on the degree of inhibitions of free radicals when tested using one or more of the procedures discussed above. In order to increase the reliability of the antioxidant results more than one protocols were used. However, in accordance with the criteria for evaluation of in vitro antioxidant activities of natural products [23, 30, 31], in this report we propose the following cutoff points;

  1. (1)

    Extracts and compounds are considered to have high or significant capacity (IC50 < 10 μg/mL for extract and IC50 < 1 μg/mL for compounds), promising activity (IC50 = 10–50 μg/mL for extract and IC50 = 5–10 μg/mL for compounds), moderate activity (IC50 = 50–100 μg/mL for extract and IC50 = 5–10 μg/mL for compounds), while sample with IC50 > 100 μg/mL for extract and > 10 μg/mL for compounds were considered to have low antioxidant capacity.

  2. (2)

    Antioxidants activities of plant extracts are considered to be very high when FRAP was > 20 mM/L, high when FRAP was 10–20 mM/L, good when FRAP was 5–10 mM/L, low when FRAP was 1–5 mM/L and very low when FRAP was below 1 mM/L.

  3. (3)

    When dealing with radical scavenging activity at a constant concentrat ion. Plant extracts were considered to exhibit low, medium, high and significant activities when their % RSA at 50 mg/mL were observed to be < 25%, 25–50%, 50–80% and > 80%, respectively.

  4. (4)

    When dealing with DPPH radical scavenging activities on the basis of degree of color changes extracts are considered to have high or significant capacity when showed strong intensity of yellow coloration, moderate when showed moderate intensity of yellow colouration, and low capacity when showed moderate intensity of yellow colouration

  5. (5)

    When dealing with Trolox equivalents (TEAC), antioxidants activities of plants extracts are considered to be very high when activities was < 0.05 and < 0.5 mmol Trolox/g in ABTS and DPPH assay, moderate at 0.05–0.20 and 0.5–1.0 mmol Trolox/g in ABTS and DPPH assay, low at 0.21–0.5 and 1.1–5.0 mmol Trolox/g in ABTS and DPPH assay, while extract with trolox equivalents > 0.5 and > 5 mmol/g in ABTS and DPPH assay respectively are considered inactive.

  6. (6)

    When dealing with in vitro hepatoprotective, plant extracts were considered to exhibit significant, medium and low hepatoprotective activities when inhibiting oxidation phenomena of > 80%, 50% and < 50% at concentration ≤ 200 μg/mL respectively

Many antioxidant compounds have been characterized form plants including flavonoids. Flavonoids are phenolic compounds with importants roles in scavenging free radicals and thus play vital roles in preventing oxidative stress associated disorders [4]. The antioxidant effects of flavonoids in biological systems are accredited to its capacity to transport electrons to free radicals, chelate metals, activate antioxidant enzymes, and reduce radicals of alpha-tocopherol or to inhibit oxidases while phenolic compounds exert it antioxidant activities by inactivating free radicals or preventing decomposition of hydroperoxide into free radicals [32]. In this review the antioxidant potential of flavonoids and other phenolic compounds have been highlighted in Table 7.

Table 7 Total phenol, total flavonoids and folic acid content of some African medicinal plants with Antioxidant potential

Evaluations of biochemical parameters including aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total proteins, albumins, bilirubins, super oxide dismutase (SOD), catalase, malondialdehyde (MDA), glutathione peroxidase have been widely used in assessing the integrity of the liver [3337]. Therefore, the hepatoprotective capacities of natural products reviewed in this study were assessed based on the levels of ameliorative effect on hapatotoxicants induced alterations in level of these biochemical parameters (Table 8).

Table 8 Hepatoprotective activity of some African medicinal plants

Antioxidant activities of extracts of plants from Western Africa

A total of 341 plants species representing 77 families from Western Africa plants were documented to have antioxidant activities (Table 1). Plant extracts from twenty five plants showed significant antioxidant capacity (IC50 < 10 μg/mL). Fourty eight extracts revealed promising antioxidant activities with IC50 values ranging from 10 to 50 μg/mL; while 59 extracts showed moderate antioxidant activities with IC50 values ranging from 50 to 100 μg/mL.

Oke and Hamburger [38] and Omale [39] presented the antioxidants activities of some medicinal plant on the basis of degree of color changes in which methanol cortex, folium and radix extract of Cnestis ferruginea, funtumia elastica, Gongronema latifolia, Sphenocentrum jollyanum, Voacanga africana and Landolfia owariensis showed strong intensity of yellow coloration in DPPH radical scavenging assay and were considered to have very high antioxidants activities, while Leea gunensis, Hedranthera barteri, Icacina trichantha, Crinum purpurascenc and Byrsocarpus coccineus revealed moderate intensity of yellow colouration. Determination of antioxidant potential on the basis of FRAP, revealed that 9 plant extracts had minimal FRAP (<1 mM/L), 37 including Althaeae radix, Foeniculi fructus, Cetrariae lichen and Phaseoli pericarpum had low FRAP (1–5 mM/L), 15 had good FRAP (5–10 mM/L) while 8 had high FRAP (10–20 mM/L) with the leaf extract of Mellisa officinalis having significant FRAP of 2.52 mM/L [30]. The extract of the leaves of Mellisa officinalis could be considered as the most suitable candidate for development into antioxidant phytomedicine. The constituent compounds should also be evaluated for their antioxidant potential. Phytochemical investigation of plants from Western Africa exhibiting antioxidant and related activities led to isolation of lophirones B (50) and lophirones C (51) (Table 6 and Fig. 3), from chloroform stem bark of Lophira alata. These two compounds show significant antioxidants activities in DPPH assay (84.4%, and 90.0% respectively at 1 μg/mL) and in vivo antioxidants activity [40]. This study shows that treatments of normal rats with 5, 10, and 20 mg/kg body of lophirones B (50) and lophirones C (51) once daily for 2 days increases the activities of ROS detoxifying enzymes (SOD, CAT, GPx, and GR) in the liver of rats when compared to the control.

Antioxidant activities of extracts of plants from Northern Africa

A total of 345 plants species representing 72 families from Northern Africa plants were documented to have antioxidant activities (Table 2). The antioxidant activities of most plant extracts originating from Northern Africa were determined using the free radical scavenging assays carried out at constant concentration of 50 mg/mL, in order to evaluate the % radical scavenging activities (RSA). Using this criteria, plant extracts were reported to exhibit low, medium, high and significant activities when their % RSA were observed to be < 25%, 25–50%, 50–80% and > 80%, respectively. Based on this criteria 39 plant extracts including; Punica granatum, Bombax malabaricum, Schefflera actinophylla, Phalangium variegate, Eucalyptus rostrata, Didonia viscose, Myrtus Communis, Tecoma capensis, Vitex trifolia, Gazania splendens, Lagerstroemia indica, Acalypha marginata, Laurus nobilis, Pelargonium oderatissimum, Khaya senegalensis and Spathodea tilotica had extremely high antioxidant power (>80% inhibition). At 5 mg/mL plant extracts of the following plants; Chrysanthemum frutesence, Aspidistra lurida, Thuja orientalis and Ruscus hyphoglossum exhibited very low antioxidant properties of < 1% RSA. In separate studies the antioxidant activities were determined at relatively higher concentration (100 mg/mL), where Capsicum annuum, Camellia sinensis, Atriplex sp., and Asphodelus microcarpus showed high % RSA [41].

Geographical locations usually influence the accumulation of secondary metabolites in most plants. Variations of these substances may be observed on different parts of the plants used in the study. Solvent systems used for extraction process may also substantially affect the composition of the extracts and hence their bioactivities [4].

The percentage (%) RSA using DPPH of the methanol and chloroform extracts of 124 Egyptian plants was evaluated at 50 mg/mL. The chloroform extracts of these plants were less active demonstrating % inhibition ranging from 0.5 to 49%; while the methanol extracts elaborating more polar compounds showed % inhibition ranging from 3 to 96 % [42].

The variations in scavenging activities of the methanol and chloroform extracts are most probably attributed to the differences in polarities of the phytochemicals [43], and also the classes of compounds extracted by the two solvents. Phytochemical investigation of some plants from Northern Africa exhibiting antioxidant and related activities led to isolation of approximately 56 compounds (Table 6 and Fig. 3). The most potent compounds included; nifedipine (47), trilinolein (42), usnic acid monoacetate (41), 5–bromosalicylaldehyde (39), naphtho [2,1–b]furan-2(1 h)- one,decahydro-3α,6,6,9α–tetramethy (38) and 2,3 dihydroxypropyl elaidate (47) (obtained from the leaf extract of Solanum nigrum) with % RSA of 78.4%, 68.5%, 74%,72.5%, 74% and 76% at 100 μg/mL respectively [44], and catechin (120) obtained from the ethyl acetate leaf extract of Hydnora abyssinica with % RSA of 68.5% at 1 mM [45]. The presence of these important compounds and the significant antioxidants power they demonstrated is an indication that these compounds, if properly screened could yield drugs of pharmaceutical significant.

Antioxidant activities of extracts of plants from Southern Africa

A total of 178 extracts from 145 plants belonging to 43 families were identified from Southern Africa (Table 3). However, the ethanol extract of the bark of Sclerocarya birrea and the leaf extract of Harpephyllum caffrum, Aspalathus lineari and Combretum apiculatum demonstrated the most significant DPPH scavenging activities with IC50 values of 2.06 ± 0.03, 2.6 ± 0.21, 3.5 ± 0.5 and 1.6 ± 0.02 μg/mL, respectively while leaf extract of Galenia africana revealed weak antioxidants activity with an IC50 value of 90.92 ± 1.2 μg/mL [46]. The antioxidant capacity of plant extracts were found to vary with the antioxidant assays used, for instance, Katerere et al. [47] reported Trolox equivalents (TEAC) per 100 g of plant material of Vigna unguiculata, Lippia javanica, Tagetes minuta, Bidens pilosa, Telfairia occidentalis and Corchorus olitarius which ranged from 0.76 to 5.77 mmol Trolox/100 g in ABTS assay and 16.29–1711.22 mmol Trolox/100 g for the DPPH assay. Similarly, Thozama [48] reported the percentage (%) inhibition of Chenopodium album, Solanum nigrum, Urtica lobulata and Amaranthus dubius ranging from 35 to 50% in DPPH assay and from 60 to 75% in ABTS assay. The difference in the antioxidant potencies among the assays was expected as each method has a unique mechanism of action under different reaction conditions [49]. For instance, ABTS+ is soluble in both aqueous and organic solvents and thus can be used to determine the antioxidant capacities of both lipophilic and hydrophilic substance [49, 50]. Viol [51] studied the antioxidants activity of 27 Zimbabwe medicinal plants extracts. Eight of these extracts exhibited antioxidant activities using DPPH with the leaves and root extracts of Rhus chirindensis and the bark of Khaya anthotheca exhibiting significant RSA of 96.9% and 96.1%, respectively. However, the roots of Dichrostachys cinerea revealed modest activities with RSA of 27.4% [51].

Antioxidant activities of extracts of plants from Central Africa

A total of 198 extracts from 166 plants belonging to 38 families originating from Central Africa, predominantly from Cameroon, have been investigated for their antioxidant potential (Table 4). The extracts that exhibited the highest antioxidant activities included; methanol extracts of the leaves and stem of Acalypha racemosa with IC50 values of 2.11 and 2.28 μg/mL, respectively; of the fruits and bark of Garcinia lucida with IC50 1.83 and 2.35 μg/mL, respectively and of the roots and bark of Hymenocardia lyrata with IC50 values of 1.96 and 1.74 μg/mL, respectively [52]. Agbor et al. [53] investigated different extracts of 42 medicinal plants for their antioxidant activities. The methanol extract of the leaves of Harungana madagascariensis, bark of Azadirachta indica, leaves of Psidium guqjava and leaf of Alchornea were considered to have the highest activities using three different assay systems for antioxidant analysis. Detailed phytochemical studies of ethnomedicinal plants from Central Africa having antioxidant activities led to isolation of approximately 62 compounds (Table 6, Fig. 3). The most active compound included; moracin T, U, S and R (8487) isolated from the bark of Morus mesozygia. These compounds revealed significant DPPH scavenging potential exhibiting IC50 values of 4.12, 5.06, 6.08 and 7.17 μg/mL, respectively [54]. Additionally, Donfack et al. [55], studied the in vitro hepatoprotective activity of six (6) compounds from methanol stem bark of Ficus gnaphalocarpa; betulinic acid (53), catechin (65), quercetin (67), quercitrin (68), epicatechin (66) and 3–methoxyquercetin (64). In this study, simultaneous treatment of hepatoma cells with these compounds exhibited antioxidants and hepatoprotective effects as judged by their ability to prevent liver cell death and LDH leakage during CCl4 intoxication. The hepatoprotection, showed by the aptitude of these molecules to preserve cellular viability and to inhibit the leakage of LDH in extracellular medium was particularly pronounced with compounds (64, 6768).

Antioxidant activities of extracts of plants from Eastern Africa

A total of 63 extracts from 51 plants belonging to 23 families were identified to exhibit antioxidant activities (Table 5). Tufts et al. [56] evaluated the ethanol extract of 13 medicinal plants for antioxidant activities using the oxygen radical absorbance capacity (ORAC) assay. Out of these extracts Mangifera indica, Psidium guajava and Ocimum americanum showed the highest antioxidant activities of 5940, 3929 and 3190 μMTE/μg respectively. These extracts also exhibited significant anti-inflammatory effect. The significant antioxidant and anti-inflammatory effect of these plants may confer hepatoprotective virtue to the plants. Detailed phytochemical studies of ethnomedicinal plants from Eastern Africa having antioxidant activities led to isolation of approximately 19 compounds (Table 6, Fig. 3). The most potents of these compounds included; rutin (13) with IC50 of 3.53 μg/ml using DPPH free radicals [57], myricitrin- based glycosides including; myricitrin (20) (IC50 = 14.2 μM), myricetin-3–O-arabinopyranoside (21) (IC50 = 15.8 μM), and quercetin-based glycosides including; quercetin-3–O-diglucosylrhamnoside (14) (IC50 = 20.7 μM) and quercetrin (19) (IC50 = 26.8 μM) [58]. The radical scavenging activities of the quercetin-based glycosides appears to be much higher than those of the kaempferol-based glycosides. This can be attributed to the presence ortho-dihydroxyl groups in the B ring of the former, which is not exemplified in the latter. Similarly, myricitrin-based glycosides which contain ortho-trihydroxy groups in the B ring were shown to be more potent scavengers than their corresponding quercetin-based glycosides. Thus, structure-activity considerations for the present series of flavonoids indicate the importance of multiple OH substitutions for antiradical action towards DPPH with ortho-trihydroxyl group in the B ring elevating the radical scavenging efficiency above that of the ortho-dihydroxyl group.

Hepatoprotective activities of extracts of plants from Africa

The liver is a vital organ which regulates many important metabolic functions and is responsible for maintaining homeostasis of the body [59]. The aetiology of liver diseases is diverse and a variety of plants has been reported to show hepatoprotective activity and so may be useful in the treatment of these diseases [25]. The mechanism of hepatic injury invariably involves peroxidation of hepatocyte membrane fatty acids causing destruction of the cells and their intracellular organelles. Oxidative stress plays a pivotal role in the initiation and progression of hepatic damage following insult to a variety of hepatotoxins [60]. These toxicants damage the hepatocyte primarily by producing reactive oxygen species which form covalent bond with the lipid moiety of the hepatic cell membranes. The drugs/chemicals and plants with antioxidant properties have been shown to protect against toxin induced hepatotoxicity through inhibition of the generation of free radicals. A list of plants reported to have significant hepatoprotective activity is shown in Table 8 in alphabetical order of their family, together with their scientific names, origin, plant part used, kind of extract used, type of assay and inducer of liver damage. Most of these planta are discussed in greater details below.

Moringa oleifera

Moringa oleifera Lam. (Moringaceae) locally known as “ben oil or drumstick tree” is a small, graceful, deciduous tree with sparse foliage [61]. The plant grows abundantly in many tropical and subtropical countries. Moringa is an ancient magic plant with a plethora of medicinal and nutritional value. The leaves, flowers, root, gums, fruit, and seed of M. oleifera have been extensively used in traditional medicine for the treatment of liver disease, lipid disorders, arthritis, and other inflammatory disorders [62]. The ethanolic extract of the leaves of M. oleifera was found to exhibit hepatoprotective effect against alcohol induced hepatotoxicity in rats [63]. This research proved that animal pretreatment with ethanolic extract of M. oleifera (300 mg/kg of weight) significantly attenuated hepatotoxin induced biochemical (serum AST, ALT, ALP, and GGT) and histopathological changes in the liver. Additionally, M. oleifera leaves also showed significant anti-inflammatory [64], and antioxidant potencies [63], [65], which may be contributing to its hepatoprotective activity. A number of phytochemicals with antioxidant activities have been characterized from Moringa oleifera including; quercetin (22), rutin (13), kaempferol and caffeoyqumic acids.

Senna alata

Senna alata (L.) Roxb) (Fabaceae) is commonly known as candle bush, with reference to the shape of its inflorescences, or ringworm tree for it traditional use. It is an annual, erect, tropical herb of 0.15 m high [66]. The leaves are well known for their medicinal used for various diseases of the liver [67]. The hepatoprotective effect of the plant has been shown in Wistar albino rat intoxicated with CCl4. This study reported that methanol extract and fractions (ethanol and butanol) of S. alata leaves administered orally at 400 mg/kg decreased hepatic enzyme levels (serum ALT, AST, ALP,) total and direct bilirubin, liver TBARS induced by CCl4 damage. Administration of the methanol extract of this plant showed maintenance of the hepatocytes membrane’s structural integrity [68]. The extract also showed strong antioxidant and anti-inflammatory [69], activities which may contribute to its hepatoprotective property.

Cochlospermum tinctorium

Cochlospermum tinctorium (Cochlospermaceae) is a bushy savannah plant, commonly found in fallow farms across northern Nigeria. It is a shrub that grows up to 10 m high [70]. Decoctions of the whole roots of C. tinctorium have been reported to be used as remedy for gonorrhoea, jaundice, gastrointestinal diseases, helminthes, bilharzias infest ations, as well as for the management of epilepsy [71]. The hepatoprotective effect of methanol extracts of C. tinctorium leaf has been studied against CCl4 induced liver injury [72]. The extract attenuated CCl4 induced rise in liver enzymes including AST and ALT, bilirubin, MDA level and prevented histopathological alterations in the liver [72]. The hepatoprotective activities of the extract have been linked to both enzymic and non-enzymic antioxidants that could bring about free radical suppressing activity.

Uvaria afzelii

Uvaria afzelii Sc Elliot (Annonaceae) is widely distributed and grown in the South and eastern part of Nigeria, where it is known by various local names such as “gbogbonishe” (Yoruba), “Umimi ofia” (Igbo) and “Osu-umimi” (Ukwani) [73]. Locally it is used in the treatment of cough, vaginal tumour, gonorrhea, jaundice, infections of the liver, kidney and bladder [74, 75]. The hepatoprotective activity of this plant was evaluatedin the experimental acute hepatic damage induced by CCl4 in rat [76]. In this study, it was reported that the methanolic extracts of the root of Uvaria afzelii, at doses of 125 mg/kg, 250 mg/kg and 500 mg/kg, significantly reduced the serum hepatic enzymes, total and un-conjugated bilirubin. Phytochemical studies of this plant has shown the presence of syncarpic acid, dimethoxym atteucinol, emorydone, 2–hydroxydemethoxym at-teucinol, uvafzelic acid, syncarpurea, afzeliindanone, flavonoids, triterpenoids and phenols [7678]. Some of these compounds have also been credited for their antiparasitic and antioxidant activities [79].

Sphenocntrum jollyanum

Sphenocntrum jollyanum Pierre (Menispermaceae) is locally known as Aduro kokoo (red medicine) and Okramankote (dog’s penis) in Ghana. It is a small erect sparsely branched rub which grows up to 1.5 m in height. Different part of S. jollyanum has been used extensively for the treatment of various ailments in Western Africa Sub-region. The methanolic extract of S. jollyanum stem bark showed significant hepatoprotective activity against CCl4 induced liver injury [80]. In addition, this extract possesses significant antioxidant activities with IC50 values of 13.11 and 30.04 μg/mL in superoxide and hydrogen radical scavenging activity, respectively [80] and anti-inflammatory [81], activities which may be contributing to its hepatoprotective effects.

Khaya grandifoliola

Khaya grandifoliola (Meliaceae) is commonly used in traditional medicine by the Bamun (a tribe of Western Cameroon) for curing liver related diseases [82]. The hepatoprotective effect of K. grandifoliola has been studied against PCM [83], and CCl4 induced hepatotoxicity [84] in rats. The methanol; methylchloride extract of the stem bark of this plant at 25 and 100 mg/kg dose dependently attenuated hepatotoxin induced alterations in biochemical parameters (serum ALP, AST, ALT and TP and liver TBARS, SOD, GSH and GR) and prevented toxin induced alteration in liver histopathology. The extract also showed antioxidant and anti-inflammatory activities [84] which may be contributing to its hepatoprotective activity.

Spathodea campanulata

Spathodea campanulata, (Bignoniaceae), it’s a widely used traditional African medicinal plant for skin diseases and stomach aches [85]. The extract of the stem bark of Spathodea campanulata produced significant hepatoprotection [86]. In this study it was reported that the methanolic extracts of the stem bark of S. campanulata, at doses of 100, 300, and 625 mg/kg significantly attenuated CCl4 induced rise in biochemical (serum AST, ALT and GGT) and histopathological changes in the liver [86]. Phytochemical studies on S. campanulata showed the presence of flavonoids, tannins, spathoside, n-alkanes, linear aliphatic alcohols, beta-sitosterol-3–O-beta-D-glucopyranoside, oleanolic acid, pomolic acid, p-hydroxybenzoic acid, phenylethanol esters, reducing sugars. The in vitro testing which gave positive results for reducing power and total phenolic content [8688], also support the activity of the plant extract with reference to its hepatoprotection.

Vernonia ambigua

Vernonia ambigua (Asteraceae) is an annual shrub growing up to 6 m high. It is widely distributed in areas like Angola, Sudan, Tanzania, Uganda and tropical Western Africa. In Nigeria it is used for gastrointestinal disorders, as a general tonic and appetite stimulant, for skin diseases and as a medication for fever, dysentery, malaria, diabetics and constipation [89]. The hepatoprotective activity of leaf extract of V. ambigua has been investigated using CCl4 induced hepatotoxicity in albino rats. The extract significantly attenuated CCl4 induced biochemical (ALT, AST and ALP, TB, CHOL, TGA, TP and ALB [90]. Plants of the genus Vernonia are known to produce characteristic compounds such as sesquiterpene lactones, with several reported biological activities, such as fungistatic [91], and cytotoxic activities [92]. The hepatoprotective properties of plants from genus Vernonia may be attributed to presence of mainly; flavonoids, steroids and polysaccharides [93], that has been characterized previously from this genus.

Ocimum americanum

Ocimum americanum (Lamiaceae) commonly known as “African basil” It is a wild herb with a distinct mint flavor, hairy leaves and scented flowers that is native to tropical Africa. The aqueous extract of O. americanum (200 and 400 mg/kg) significantly attenuated PCM induced biochemical (serum ALP, AST, ALT and TBIL level) and histopathological alterations in the liver [94]. The hepatoprotective activity of Ocimum americanum may be attributed to its antioxidant activities [95].

Tulbaghia violacea

Tulbaghia violacea (Alliaceae) is a fast-growing, bulbous plant that reaches a height of 0.5 m. In the Eastern Cape of South Africa rhizomes of Tulbaghia violacea has been used for the treatment of jaundice, gall bladder stones, liver diseases and heart disease [96]. The rhizomes extract of T. violacea dose dependently attenuated atherosclerogenic induced alteration in markers of endothelial dysfunction, lipid profile, liver enzymes and histological changes [97]. The antioxidant and cytotoxicity activities of T. violacea as well as its phytochemical components such flavonoids and saponins [98] may be responsible for its hepatoprotective properties.

Irvingia gabonensis

Irvingia gabonensis (Irvingiaceae) locally known as “bush mango or African mango” since the trees bear fruits that look like small mango (Matos et al., 2010). In Senegal, the decoction of the stem bark is used in the treatment of gonorrhoea, hepatic and gastrointestinal disorders [99]. The thanol extract of the leaves of this plant has been investigated for its hepatoprotective activity in sodium arsenite (SA) induced hepatotoxicity and clastogenicity in male Wistar rats [100]. The extract at 250 or 500 mg/kg dose dependently attenuated sodium arsenite induced rise in liver enzymes including AST, ALT and and gamma glutamyltransferase (γGT) and prevented histopathological alterations in the liver [100]. Phytochemical studies on the ethanol extract of Irvingia gabonensis showed the presence of of tannins, saponins, alkaloids, terpenoids, flavonoids and phenols [100]. Tannins have been reported to have anti-inflammatory and antiulcer property in rodents and they also exhibit strong antioxidant properties [101].

Echinops galalensis

The methanol extract of the flowering aerial parts of Echinops galalensis (Asteraceae), its fractions and the isolated compounds (2533) have been reported for their hepatoprotective effects agaisnt CCl4 induced cell damage in an in vitro assay on human hepatoma cell line (Huh7). The extract and isolated compounds (2533) at 100 μg/mL prior to CCl4 challenge protected against cell injury by decreasing the level of AST, ALT, MDA and increasing the activities of SOD [102]. The protective effects of E. galalensis methanolic extract, its fractions as well as the isolated compounds is at least partly due to their antioxidant activities as evidenced by the reduction in MDA level and the increase in SOD activity.

Lawsonia inermis

Lawsonia inermis (lythraceae) is a shrub or small tree cultivated in many regions as an ornamental and commercial dye crop [103]. It is mostly found in the tropic, sub-tropic, and semi-arid zones of Africa (tropical Savannah and tropical arid zones), South Asia and North Australia [104]. As a medicinal plant, the leaves, seed and bark of L. inermis have been used in folk remedy as astringent, hypotensive, sedative, and against a headache, jaundice, spleen enlargement, leprosy and other liver disease [105]. Its hepatoprotective activity was shown in a toxicity model by CCI4 in rats. These research proved that animal pretreatment with a methanolic extract of Lawsonia inermis (100 and 200 mg/kg of weight) attenuated the increase in AST serum activity, alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), and histological changes observed in the damage induced by CCl4 [106, 107]. Previous reports have shown that L. inermis is rich in phenolic compounds such as phenolic acids, flavonoids, tannins, lignin, and others that possess antioxidant, anticarcinogenic, and antimutagenic effects as well as antiproliferative potentials [108], which may be responsible for its hepatoprotective activities.

Ficus chlamydocarpa

Ficus chlamydocarpa (Moraceae) is traditionally used in Cameroon for the management of different diseases including; filarial, diarrheal infections and tuberculosis [109]. Another ethnopharmacological survey has revealed that a decoction of the stem bark is used in West Cameroon folk medicine for the treatments of abdominal problems, arthritis, inflammatory conditions and jaundice, which are commonly considered symptomatic of liver-related diseases.

Its hepatoprotective effect was evaluated through the induction of acute hepatic damage in rats using CCl4 [99]. In this, study the pre-treatment with 50–200 mg/kg of methanolic extract of F. chlamydocarpa stem bark prevented serum increase of hepatic enzyme markers and lactate dehydrogenase (LDH), enhanced hepatic reduced glutathione (GSH) level and decreased of hepatic malondialdehyde (MDA) during CCl4 intoxication. Previous phytochemical studies on stem bark of F. chlamydocarpa revealed the presence of the following flavonoids; alpinumisoflavone (115), genistein (4′, 5, 7– trihydroxyisoflavone 116) and luteolin (3′, 4′, 5, 7– tetrahydroxy flavones 117) with significant DPPH radical scavenging activities with IC50 (μg/mL of 6, 5.7, 5.0 respecively [99].

Allanblackia gabonensis

Allanblackia gabonensis (Guttiferae) is commonly grown in tropical Africa including; Cameroon, Democratic Republic of Congo, etc. between around 500 and 1750 m above sea level [110]. The plant is used in traditional medicine to treat some inflammatory diseases. The aqueous suspension of the stem bark of A. gabonensis showed significant hepatho-nephroprotective activity against acetaminophen-induced liver and kidney disorders in rats. In this, study the pre-treatment with 100 and 200 mg/kg significantly reduced the serum level of MDA, increase in enzymatic antioxidant activities (SOD and CAT) and non enzymatic antioxidant (GSH) levels [111]. The stem bark of this plant has been known to elaborate the following compounds xanthones, benzophenone, flavonoide, and phytosterol [112]. In addition, A. gabonensis possess significant analgesic and anti-inflammatory activities [113] which may be contributing to its hepatoprotective activities.

Ficus exasperata

Ficus exasperata vahl (Moraceae) is a terrestrial plant that grows 20 m high and inhabits the evergreen and secondary rainforest of West Africa. The plant is commonly known as sand paper tree, it is also known locally as “anwerinwa” [114]. The ethanol extracts of the leaves of F. exasperata showed significant hepatoprotective activitie in acetaminophen-induced hepatotoxic rats [115]. The extract at 125–500 mg/kg significantly ameliorated toxin induced alterations in the liver ALT, AST, ALP and bilirubin levels. The histological evaluation showed a partial prevention of inflammation, necrosis and vacuolization induced by CCl4 [115].

Erythrina senegalensis

Erythrina senegalensis DC (Fabaceae), locally known by the Bamun people in Cameroon as ‘Megham njû’ is a thorny shrub or small tree, with a corky stem bark and bright red flowers, found in Sudanese savannah regions. Hepatoprotective effect of the ethanolic extract of Erythrina senegalensis stem bark was studied in vivo against CCl4−induced induced liver damage as well as in vitro against rat liver slices intoxicated CCl4. E. senegalensis extract at 100 mg/kg significantly attenuated hepatotoxin induced biochemical serum ALT, AST and lipid peroxidation in liver homogenate. Polyphenols including flavonoids have been characterized from this plants which could be implicated for its hepatoprotective potential [116].

Njayou et al. [117], evaluated the hepatoprotective effect of fifty four Cameroonian plants extracts against Fe (II)-Ascorbate induced microsomal lipid peroxidationin rat liver. Only 15 plants extract inhibiting oxidation phenomena with percentage inhibition of > 50 at 200 μg/mL were considered as possessing a high lipid oxidation inhibitory potential. In this respect, Mangifera indica, Enantia chlorantha, Voacanga africana, Aspilia africana, Senna alata, Piliostigma thonningii, Piliostigma thonningii, Kalonchoe crenata, Alchornea laxiflora, Crotalaria lachnophora, Erythrina senegalensis, Khaya grandifoliola, Entada africana, Melinis minutiflora and Curcuma longa were found to be active. Among these active plant species, some of them, namely E. chlorantha [118], E. africana [119] and C. longa [120], have been reported to be active against experimentally induced hepatitis. M. indica on its part has been shown to be very effective against lipid and protein oxidation in vitro and injury associated to hepatic ischemia reperfusion [121, 122]. The inhibitory effect against the free radical-mediated degradation of microsomal lipid peroxidation by plant extracts mentioned above may also be attributed to flavonoids and polyphenols as many of these phytoconstituents are known to be antioxidants [123]. The presence of flavonoids and polyphenols has been reported in all the above cited plant extracts [124, 125].

Aja et al., [2], documented the antioxidant activities of the ethanol leaf extracts of C. citratus and H. spicigera against Plasmodium berghei induced oxidative stress by significantly (P < 0.05) increasing the superoxide dismutase, reduced glutathione, catalase and peroxidase activities and decreasing the lipid peroxidation when compared with the controls. This study indicates the effectiveness of the use of Cymbopogon citratus and Hyptis spicigera in the management of oxidative stress caused by malaria [2].

Mulata et al. [126], evaluated the effect of hydroethanolic seed extract of Calpurnia aurea against highly active antiretroviral therapy (HAART) induced free radical reactions in the liver and liver cell damage in rats. The authors reported that the extract (300 mg/kg) reduced the HAART induced liver toxicity by decreasing the free radical reactions, ALP, ALT, AST release and increasing antioxidant profiles in treated rats.

A polyherbal formulation comprising of Gongronema latifolia, Ocimum gratissimum and Vernonia amygdalina demonstrated significant hepatoprotective activities by attenuating the increase in serum hepatic enzyme levels after CCl4 treatment compared to the toxin control group and increasing the levels of serum CAT, GPx, GSH, GST, SOD, total protein and significantly (p < 0.05) decreasing lipid peroxidation compared to the toxin control group [127].

“Ata-Ofa” a polyherbal formulation consisting of twenty one (21) plant products, including, Ginger officinalle, Tamarindus indica, Khaya senegalensis, Moringa oleifera, Nauclea latifolia, Camellia sinensis, Anacardium occidentale, Aframomum melegueta, Phyllantus amarus, Morinda lucida and Mangifera indica was reported (at 5 mg/kg) for in vivo antioxidant, hepatoprotective and curative effects by its ability to ameliorate CCl4 induced alterations in biochemical parameters and antioxidants enzymes in intoxicated rat [128].

Antioxidants and hepatoprotective activities of insect/mollusk and their secreations

Omalu et al. [129], evaluated the free radical scavenging activity of Nigeria Leech (Aliolimnatis michaelseni) saliva extract. Their results revealed that the extract excert significant DPPH free radical scavenging activity with IC50 value of 8.169 μg/mL initially and 8.67 μg/mL after starvation for 1 month. Omalu et al., [130], also documented the antioxidants potency of maggots of the blowfly (Lucilia robineau) excretion/saliva extract with DPPH free radical scavenging activity of (IC50 of 152.66 μg/mL) compared with 108.99 μg/mL of L-ascorbic.

Giant African Snail (Achachatina maginata) haemolymph has been reported for in vitro antioxidant activity with an IC50 value of 579.66 ± 2.69 μg/mL in DPPH radical scavenging assay and 310.75 ± 3.12 μg/mL in lipid peroxidation inhibitory assay. The haemolymph also excert ameliorative effects on CCL4−induced elevations of the levels of AST, ALT, ALP, TBARS and it reversal effect on reduced concentration of catalase induced by CCL4 intoxication. The total phenolics and flavonoids contents were reported to be 9.30 ± 0.11 mg/g GAE and 15.20 ± 0.59 mg/g catechin equivalent respectively [5].

Shittu et al. [131], reported the ameliorative effects of the methanol extracts of Musca domestica (400 mg/kg) against T. brucei induced alteration in antioxidants enzymes (SOD and CAT). Antioxidant screening of the extract against DPPH was positive, with IC50 and antioxidant activities index (AAI) of 174.38 mg/mL and 0.29 respectively. Since oxidative stress has been implicated in the etiology of African trypanosomiasis, these two findings suggest that the methanol extract of Musca domestica probably excert it anti-trypanosoma effect by free radical scavenging and thus could serve as a candidate for the development of new drugs for the treatment of trypanosomiasis. The methanol extracts of Nigeria bee propolis (600 mg/kg) has been reported for hepatocurative effect by ameliorating CCL4−induced alterations in the serum and liver AST, ALT and ALP activities when administered orally to rats for 10 days [132].

Tanzania honey bee has been reported for DPPH radical scacvenging activity with IC50 4.19, 12.93 and 18.03 mg/mL in stingless bee honeys, raw bees honey and processed bees honey respectively. Similarly, iron chelating activities were reported with IC50 value of 0.04, 0.057 and 0.158 mg/mL for stingless bee’s honey, raw bee’s honey and processed bee’s honey respectively [133]. Previous phytochemical investigation of the Nigerian sweet and bitter honey revealed total flavonoids contents of 20.81 μg/mL and 18.92 μg/mL respectively [134].

Nyanzi et al., [135], reported the antioxidant activities of methanol extract from freeze-dried cells of probiotic Lactobacillus strains. At the extract concentration of 20 mg/mL the authors reported that Lb. acidophilus, Lb. rhamnosus and Lb. casei strains had DPPH scavenging activities of 77.9–86.1%, 45.7–86.4% and 36.9–45.8% respectively. This finding is an indication that Probiotic extracts can potentially be used as bio-preservatives and in reduction of oxidative stress.

Conclusion and future prospects

Meta-analysis of available scientific literature on antioxidants and hepatoprotective activity of African natural products to a great extent validate folkloric claims about the usefulness of these botanicals to treat liver diseases and other oxidative stress induced disorder. This review has documented the list of African natural products with potential antioxidants and hepatoprotectives effect. Many of these natural products displayed good antioxidants and hepatoprotective activities. This explains the effort of Africa research institutes in drug discovery from natural products. However, the variations in method of analsis, presentations of results, doses, durat ion as well as the geographical difference of the plants reviewed in this study has made it difficult to accurately point out plant/compounds with the best reported antioxidants and hepatoprotective activities. But our close analysis of the reports seem to suggest that Combretum apiculatum, Telfaria occidentalis, Acalypha racemosa, Garcinia lucida, Xeoderris sthulmannii, Clausena anisata, Harpephyllum caffrum, Ceratotheca sesamoides, Camellia sinensis, Cyathea dregei, Harpephyllum caffrum, Aspalathus linearis were the most active ROS-detoxifying plant extracts from African flora. The best ROS-detoxifying phytochemicals were moracin T, U, S and R (8487), oleanolic acid (54), 5,7,4′–trihydroxy–3,8,3′,5′–tetramethoxyflavone (89), 5,7,3′–trihydroxy–3,8,4′,5′-trimethoxyflavone (88), luteolin (3′,4′,5,7–tetrahydroxy flavone) (117) and genistein (4′,5,7– trihydroxyisoflavone) (116). It is hoped that pertinent scientist and stakeholders will look further into some of these plants and compounds for detailed authentification and subsequent commercialization. Although, most of studies reviewed are preliminary in nature, detailed isolation, characterization, mechanisms of actions of these of isolated compounds, safety studies, quality control as well as clinical trials on some of these herbs and their isolated compounds is far from satisfactory.



2,2′-azinobis-3–ethylbenzothiozolin-6–sulfonic acid


Alkaline phosphatase


Alanine aminotransferase


Aspartate amino transferase





CCL4 :

Carbon tetrachloride

CH2Cl2 :




CO(CH3)2 :





Direct bilirubin




Ethyl acetate




Ferric nitrilotriacetate


Fe2+ chelating ability and ferric reducing antioxidant properties


Glutathione peroxidase

H2O2 :

Hydrogen peroxide


Low density lipoprotein






Microsomal lipid peroxydation

n-C6H12 :



Nitric oxide


Hydroxyl ion


Oxygen radical absorbance capacity




Reducing power capacity


Superoxide dismutase


ß-Carotene-linoleic acid model system


Total bilirubin


Trolox equivalent antioxidant capacity




Very low density lipoprotein


  1. 1.

    Okoli JTN, Agbo MO, Ukekwe IF. Antioxidant and Hepatoprotective Activity of Fruit Extracts of Tetrapleura tetraptera (Schum & Thonn) Taubert. Jordan Journal of Biological Sciences. 2014;7(4):251–4.

    Article  Google Scholar 

  2. 2.

    Aja PM, Odeh CO, Uraku AJ, Offor CE. Evaluation of antioxidant activities of ethanol leaf extracts of Cymbopogon citratus and Hyptis spicigera in mice exposed to Plasmodium berghei. Int J Curr Microbiol App Sci. 2015;4:382–93.

    Google Scholar 

  3. 3.

    Bjelakovic G, Nikolova D, Gluud LL, Simonetti RG, Gluud C. Mortality in randomized trials of antioxidant supplements for primary and secondary prevention: systematic review and Meta-analysis. J Am Med Assoc. 2007;297:842–57.

    CAS  Article  Google Scholar 

  4. 4.

    Lawal B, Ossai PC, Shittu OK, Abubakar AN. Evaluation of phytochemicals, proxim at e, minerals and anti-nutritional compositions of yam peel, maize chaff and bean coat. Int J Appl Biol Res. 2014;6:21–37.

    Google Scholar 

  5. 5.

    Lawal B, Shittu OK, Ossai PC, Abubakar AN, Ibrahim AM. Antioxidant activities of giant African snail (Achachatina maginata) haemolymph against CCl4− induced hepatotoxicity in albino rats. Brit J pharm Res. 2015;6:141–54.

    CAS  Article  Google Scholar 

  6. 6.

    Souri E, Amin G, Farsam H, Barazandeh TM. Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts. Daru. 2008;16:83–7.

    Google Scholar 

  7. 7.

    Anderson JW, Smith BM, Wasnock CS. Cardiovascular and renal benefits of dry bean and soyabean intake. Amer J Clin Nutr. 1999;70:464–74.

    Google Scholar 

  8. 8.

    Ndhlala A, Ncube B, Van Staden J. Antioxidants versus reactive oxygen species – A tug of war for human benefits? In: Laher I, editor. Systems Biology of Free Radicals and Antioxidants. Berlin Heidelberg: Springer; 2014. p. 3987–4002.

    Google Scholar 

  9. 9.

    Lampe JW. Spicing up a vegetarian diet: chemopreventive effects of phytochemicals. Amer J Clin Nutr. 2003;78:579S–83S.

    CAS  PubMed  Google Scholar 

  10. 10.

    Patience OO, Festus BCO, Philip FU, Nneka RN, Ijeoma EA, Nkemakonam CO. Phytochemical analysis, Hepatoprotective and antioxidant activity of Alchornea cordifolia Methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats. Asian Pac J Trop Med. 2012;5:289–93.

    Article  Google Scholar 

  11. 11.

    Ibrahim M, Khaja MN, Aara A. Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: in vitro and in vivo studies. World J Gastroenterol. 2008;14:2566–71.

    PubMed  PubMed Central  Article  Google Scholar 

  12. 12.

    Lawal B, Shittu OK, Oibiokpa IF, Mohammed H, Umar SI, Haruna GM. Antimicrobial evaluation, acute and sub-acute toxicity studies of Allium sativum. J Acute Dis. 2016. doi:10.1016/j.joad.2016.05.002.

    Google Scholar 

  13. 13.

    Rajkapoor BY, Venugopal J, Anbu N, Harikrishman MG, Ravichandran V. Protective effect of phyllanthus polyphllus on acetaminphen induced hepatotoxicity in rats. Pak JPharm Sci. 2008;21:90–3.

    Google Scholar 

  14. 14.

    Jyothi KSN, Hemalatha P, Avanthi A, Challa S. A comparative analysis on the alpha amylase inhibitory potential of six ornamented medicinal plants. J Nat Prod Plant Res. 2013;3:1–6.

    Google Scholar 

  15. 15.

    Ostapowicz G, Fontana RJ, Schiodt FV, Larson A, Davern TJ, Han SH, et al. Results of a prospective study of acute liver failure at 17 tertiary care centers in the united st at es. Annals Internal Med. 2002;137:947–54.

    Article  Google Scholar 

  16. 16.

    Kodavanti RR, Joshi UM, Young YA, Meydrech EF, Mehendale HM. Protection of hepatotoxic and lethal effects of CCl4. Toxicological Pathology. 1989;17:494–505.

    CAS  Google Scholar 

  17. 17.

    Arhoghro EM, Ekpo KE, Anosike EO, Ibeh GO. Effect of aqueous extract of bitter leaf (Vernonia Amygdalina Del) on carbon tetrachloride (CCl4) induced liver damage in albino wistar rats. Eur J Scientific Res. 2009;26:122–30.

    Google Scholar 

  18. 18.

    Pramyothin P, Ngamtin C, Poungshompoo S, Chaichantipyuth C. Hepatoprotective activity of Phyllanthus amarus Schum. et. Thonn. extract in ethanol tre at ed rats: in vitro and in vivo studies. J Ethnopharmacol. 2007;114:169–73.

    CAS  PubMed  Article  Google Scholar 

  19. 19.

    Bashir L, Shittu OK, Sani S, Busari MB, Adeniyi KA. African natural products with potential antitrypanosoma properties: A review. Int J Biochem Res Rev. 2015;7:45–79.

    CAS  Article  Google Scholar 

  20. 20.

    Manach C, Morand C, Demigne C, Texier O, Regerat F, Remesy C. Bioavailability of rutin and quercetin in rats. FEBS Lett. 1997;409:12–6.

    CAS  PubMed  Article  Google Scholar 

  21. 21.

    Nafiu MO, Salawu MO, Kazeem MI. Antioxidant activity of African medicinal plants. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 787–803.

    Google Scholar 

  22. 22.

    Atawodi SE. Antioxidant potential of African medicinal plants. Afri J Biotechnol. 2005;4:128–33.

    Google Scholar 

  23. 23.

    Kuete V, Efferth T. Cameroonian medicinal plants: pharmacology and derived natural products. Front Pharmacol. 2010;1:1–19.

    Article  Google Scholar 

  24. 24.

    Hamzah, R.U., Jigam, A.A., Makun H.A., Egwim, E.C. (2013a). Antioxidant Properties of Selected African Vegetables, Fruits and Mushrooms: A Review. Intech.

  25. 25.

    Adewusi EA, Afolayan AJ. A review of natural products with hepatoprotective activity. J Med Plant Res. 2010;4:1318–34.

    Google Scholar 

  26. 26.

    Adebayo JO, Krettli AU. Potential antimalarials from Nigerian plants: A review. J Ethnopharmacol. 2011;133:289–302.

    CAS  PubMed  Article  Google Scholar 

  27. 27.

    Lawal B, Shittu OK, Kabiru AY, Jigam AA, Umar MB, Berinyuy EB, et al. Potential antimalarials from African natural products: A review. J Intercult Ethnopharmacol. 2015;4:318–43. doi:10.5455/jice.20150928102856.

    PubMed  PubMed Central  Article  Google Scholar 

  28. 28.

    Chanda S, Dave R. In vitro models for antioxidant activity Evaluation and some medicinal plants possessing antioxidant properties: An overview. Afri J Microbiol Res. 2009;3:981–96.

    Google Scholar 

  29. 29.

    Ndhlala A, Moyo M, Van Staden J. Natural antioxidants: Fascinating or mythical biomolecules? Molecules. 2010;15:6905–30.

    CAS  PubMed  Article  Google Scholar 

  30. 30.

    Katalinic V, Milos M, Kulisic T, Jukic M. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem. 2006;94:550–7.

    CAS  Article  Google Scholar 

  31. 31.

    Omisore NOA, Adewunmi CO, Iwalewa EO, Ngadjui BT, Adenowo TK, Abegaz BM, et al. Antitrichomonal and antioxidant activities of Dorstenia barteri and Dorstenia convexa. Braz J Med Biol Res. 2005;38:1087–94.

    CAS  PubMed  Article  Google Scholar 

  32. 32.

    Ramde-Tiendrebeogo A, Tibiri A, Hilou A, Lompo M, Millogo-Kone H, Nacoulma OG, et al. Antioxidative and antibacterial activities of phenolic compounds from Ficus sue Forssk. Inter J Biol Chem Sci. 2012;6(1):328–36.

    Google Scholar 

  33. 33.

    Bashir L, Shittu OK, Busari MB, Sani S, Aisha MI. Safety Evaluation of Giant African land Snails (Archachatina marginata) haemolymph on hematological and biochemical parameters of albino rats. J Adv med Pharm Sci. 2015;3:122–30.

    Article  Google Scholar 

  34. 34.

    Lawal B, Shittu OK, Abubakar AN, Umar MB, Ibrahim AM, Haruna GM. Biochemical evaluation in Wister rats (Rattus novergicus) following chronic exposure of methanol leaf extract of Telfairia occcidentalis. J Pharm Biomed Sci. 2015;5:740–4.

    CAS  Google Scholar 

  35. 35.

    Shittu OK, Lawal B, Abubakar NA, Berinyuy BE, Busari MB, Ibrahim AO. Toxicological Implications of Methanol Extract from Nigerian Bee Propolis on Some Selected Rat Tissues. J Pharm Biomed Sci. 2015;05:524–31.

    Google Scholar 

  36. 36.

    Shittu OK, Lawal B, Blessing Uchenna AB, Haruna GM, Abubakar AN, Berinyuy EB. Alteration in Biochemical Indices Following Chronic Administration of Methanolic Extract of Nigeria Bee Propolis in Wister Rats. Asian Pac J Trop Dis. 2015;5:654–7.

    Article  Google Scholar 

  37. 37.

    Yusuf OK, Bewaji CO, Ekanem JT. Biochemical Evaluation of Fermented Wheat Germ Extract on Trypanosoma brucei -infected rats. Afr J Biomed Res. 2010;13:219–24.

    Google Scholar 

  38. 38.

    Oke JM, Hamburger MO. Screening of some Nigerian medicinal plants for antioxidant activity using 2, 2, Diphenyl-picryl-hydrazyl radical. Afri J Biom Res. 2002;5:77–9.

    Google Scholar 

  39. 39.

    Omale J. Cytotoxicity and Antioxidant Screening Of Some Selected Nigerian Medicinal Plants. Asian J Pharm Clin Res. 2009;2:48–53.

    Google Scholar 

  40. 40.

    Ajiboye TO, Yakubu MT, Oladiji AT. Cytotoxic, Antimutagenic, and antioxidant activities of methanolic extract and chalcone dimers (Lophirones B and C) derived from Lophira alata (Van Tiegh. Ex Keay) stem bark. J Evid Based Complementary Altern Med. 2014;19:2.

    Article  CAS  Google Scholar 

  41. 41.

    Aboul-Enein AM, El-Ela FA, Shalaby EA, El-Shemy HA. Traditional medicinal plants research in Egypt: Studies of antioxidant and anticancer activities. J Med Plant Res. 2012;5:689–703.

    Google Scholar 

  42. 42.

    Moussa AM, Emam AM, Diab YM, Mahmoud ME, Mahmoud AS. Evaluation of antioxidant potential of 124 Egyptian plants with emphasis on the action of Punica gran at um leaf extract on rats. Int Food Res J. 2011;18:535–42.

    CAS  Google Scholar 

  43. 43.

    Sudjaroen Y, Haubner R, Wurtele G, Hull WE, Erben G, Spiegelhalder B, et al. Isolation and structure elucidation of phenolics antioxidants from Tamarind (Tamarindus indica L) seeds and pericarp. Food Chem Toxicol. 2005;43(11):1673–82.

    CAS  PubMed  Article  Google Scholar 

  44. 44.

    Aboul-Enein AM, El-Ela FA, Shalaby E, El-Shemy H. Potent Anticancer and Antioxidant Activities of Active Ingredients Separated from Solanum nigrum and Cassia italica Extracts. J Arid Land Stud. 2014;24:145–52.

    Google Scholar 

  45. 45.

    Koko WS, Osman E, Galal M. Antioxidant and antiglyCAT ion potential of some Sudanese medicinal plants and their Isolated compounds. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas. 2009;8:402–11.

    Google Scholar 

  46. 46.

    Sharma R, Lall N. Antibacterial, antioxidant activities and cytotoxicity of plants against Propionibacterium acnes. South Afri J Sci. 2014;110:8.

    Google Scholar 

  47. 47.

    Katerere DR, Graziani G, Thembo KM, Nyazema NZ, Ritieni A. Antioxidant activity of some African medicinal and dietary leafy African vegetables. Afr J Biotechnol. 2012;11:4103–8.

    CAS  Google Scholar 

  48. 48.

    Thozama, K.M. Phytochemical and Antioxidant Composition of Selected Local Wild Plants in South Africa: Considerat ion of Alternative Nutrients for Health Promotion. 2015 5th International Conference on Biomedical Engineering and Technology IACSIT Press, Singapore; 2015. V81. 17. DOI: 10.7763/IPCBEE

  49. 49.

    Arnao MB. Some Methodological Problems in the Determination of antioxidant activity using chromogen radicals: A Practical Case. Trends Food Sci Tech. 2000;11:419–21.

    CAS  Article  Google Scholar 

  50. 50.

    Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2006;19:669–75.

    CAS  Article  Google Scholar 

  51. 51.

    Viol DI. Screening of traditional medicinal plants from Zimbabwe for phytochemistry, antioxidant, antimicrobial, antiviral and toxicological activities. Harare: Master Thesis submitted to School of Pharmacy College of Health Sciences University of Zimbabwe; 2009.

    Google Scholar 

  52. 52.

    Sylvie DD, Anatole PC, Cabral BP, Veronique BP. Comparison of in vitro antioxidant properties of extracts from three plants used for medical purpose in Cameroon: Acalypha racemosa, Garcinia lucida and Hymenocardia lyrata. Asian Pac J Trop Biomed. 2014;4:625–S32.

    Article  Google Scholar 

  53. 53.

    Agbor GA, Kuate A, Oben JE. Medicinal plants can be good source of antioxidant: Case study in Cameroon. Pak J Biol Sci. 2007;10:537–44.

    CAS  PubMed  Article  Google Scholar 

  54. 54.

    Kapche GDWF, Fozing CD, Donfack JH, Fotso FW, Amadou D, Tchana AN, et al. Moracin Q–U, new antioxidant prenylated arylbenzofuran derivatives from Morus mesosygia. Phytochemistry. 2009;70:216–21.

    CAS  PubMed  Article  Google Scholar 

  55. 55.

    Donfack JH, Amadou D, Ngueguim TF, Kapche DWFG, Tchana NA, Buonocore D, et al. In vitro hepatoprotective and antioxidant activities of crude extract and isolated compounds from Ficus gnaphalocarpa. Inflammopharmacol. 2011;19:35–43. doi:10.1007/s10787-010-0070-4.

    Article  CAS  Google Scholar 

  56. 56.

    Tufts H. R, Harris CS, Bukania Z. N, Johns T. Antioxidant and Anti-Inflammatory Activities of Kenyan Leafy Green Vegetables, Wild Fruits, and Medicinal Plants with Potential Relevance for Kwashiorkor. Evid Based Complementary Altern Med. 2015,

  57. 57.

    Sintayehu B, Asres K, Raghavendra Y. Radical scavenging activities of the leaf extracts and a flavonoid glycoside Isolated from Cineraria abyssinica Sch. Bip Exa Rich J Appl Pharm Sci. 2012;02:44–9.

    Article  CAS  Google Scholar 

  58. 58.

    Asres K, Gibbons S, Bucar F. Radical Scavenging Compounds from Ethiopian Medicinal Plants. Ethiop Pharm J. 2006;24:23–30.

    CAS  Article  Google Scholar 

  59. 59.

    Enas AKM. Hepatoprotective effect of aqueous leaves extract of Psidium guajava and Zizyphusspina-christi against paracetamol induced hepatotoxicity in rats. J Appl Sci Res. 2012;8:2800–6.

    Google Scholar 

  60. 60.

    Al-Asmari AK, Al-Elaiwi AM, Athar MT, Tariq M, Al Eid A, Al-Asmary SM. A Review of hepatoprotective plants used in Saudi traditional medicine. Evid Based Compl Altern Med. 2014;2014:1–22.

    Article  Google Scholar 

  61. 61.

    Fuglie, L. J. The Moringa Tree: a local solution to malnutrition? Church World Service in Senegal. 2005. pp75

  62. 62.

    Biswas SK, Chowdhury A, Das J, Roy A, ZHosen SM. Pharmacological potentials of Moringa oleifera Lam.: A review. Inter J Pharm Sci Res. 2012;3:305–10.

    Google Scholar 

  63. 63.

    Saalu LC, Ogunlade B, Ajayi GO, Oyewopo AO, Akunna GG, Ogunmodede OS. The hep at o-protective potentials of Moringa oleifera leaf extract on alcohol-induced hepato-toxicity in wistar rat. Am J Biotechnol Mol Sci. 2012;2:6–14.

    Article  Google Scholar 

  64. 64.

    Kurma SR, Mishra SH. Antiinflammatory and Hepatoprotective activities of fruits of Moringa. Ind J Nat Prod. 1998;14:3–10.

    Google Scholar 

  65. 65.

    Kumar NA, Pari L. Antioxidant action of Moringa oleifera Lam. (drumstick) against antitubercular drugs induced lipid peroxidationin rats. J Med Food. 2003;6:255–9.

    CAS  Article  Google Scholar 

  66. 66.

    Yakubu MT, Musa IF. Liver and kidney functional indices of pregnant rats following the administration of the crude alkaloids from Senna alata (linn. roxb) leaves. Irani J Toxicol. 2012;6:615–25.

    CAS  Google Scholar 

  67. 67.

    Adedayo O, Anderson WA, Moo-Young M, Snieckus V, Patil PA, Kolawole DO. Kinetics of antibacterial activity and physicochemical damage caused by the extracts of Senna alata flowers. Pharma Biol. 2002;40:461–5.

    Article  Google Scholar 

  68. 68.

    Patrick-Iwuanyanwu KC, Iwuanyanwu P, Matthew O, Makhmoor T. Hepatoprotective effect of crude methanolic extract and fractions of Ring worm plant Senna alata (L. Roxb) leaves from Nigeria against carbon tetrachloride –induced hepatic damage in rats. Europ J Exp Biol. 2011;1:128–38.

    Google Scholar 

  69. 69.

    Hennebelle T, Weniger B, Joseph H, Sahpaz S, Bailleul F. Senna alata. Fitoterapia. 2009;80:385–93.

    PubMed  Article  Google Scholar 

  70. 70.

    Traore M, Guiguemde A, Yago I, Nikiema JB, Tinto H, Dakuyo ZP, et al. Investigation of antiplasmodial compounds from two plants, Cochlospermum tinctorium A, Rich and Gardenia sokotensis. Afr J Tradit Comp Alt Med. 2006;4:34–41.

    Google Scholar 

  71. 71.

    Mann A, Gbate M, Umar AN. Phytomedicine. In: Evans JEA, editor. Medicinal and Economic plants of Nupe Land. Nigeria: Bida; 2003. p. 64.

    Google Scholar 

  72. 72.

    Akinloye OA, Ayankojo AG, Olaniyi MO. Hepatoprotective Activity of Cochlospermum tinctorium against carbon tetrachloride induced hepatotoxicity in rats. Rom J Biochem. 2012;49:3–12.

    Google Scholar 

  73. 73.

    Odugbemi T. Medicnal plants as antimicrobials. In: Outlines and pictures of Medicinal plants from Nigeria. Lagos: University of Logos press; 2006. p. 53–64.

    Google Scholar 

  74. 74.

    Gill LS. Ethnomedical Uses of Plants in Nigeria. Benin City: University of Benin Press; 1989. p. 134.

    Google Scholar 

  75. 75.

    Kayode J, Ige OE, Adejogo TA, Igbakin AA. Conservation and biodiversity erosion in Ondo State, Nigeria (3): Survey of plant barks used in native pharmaceutical extraction in Akoko region. Ethnobotanical leaflet. 2011;13:655–67.

    Google Scholar 

  76. 76.

    Ofeimun JO, Eze GI, Okirika OM, Uanseoje SO. Evaluation of the Hepatoprotective effect of the methanol extract of the root of Uvaria afzelii (Annonaceae). J Appl Pharma Sci. 2013;3:125–9.

    Google Scholar 

  77. 77.

    Hufford CD, Oguntimein B, Martin M, Clardy J. Syncarpurea; a novel metabolite from U. afzelii. Tetrahedron Lett. 1984;25(4):371–4.

    CAS  Article  Google Scholar 

  78. 78.

    Okpekon T, Millot M, Champy P, Gleye C, Yolou S, Bories C, et al. A Novel I-indanone isolated from Uvaria afzelii roots. Nat Prod Res. 2009;23(10):909–15.

    CAS  PubMed  Article  Google Scholar 

  79. 79.

    Mehta RS, Shankar MB, Geetha M, Saliyu AK. Hepatoprotective activity of rianthema portulacastrum. Indian Drugs. 1999;36:241–4.

    Google Scholar 

  80. 80.

    Olorunnisola OS, Bradley G, Afolayan AJ. Ethnobotanical inform at ion on plants used for the management of cardiovascular diseases in Nkonkobe municipality of South Africa. J Med Plants Res. 2011;5:4256–60.

    Google Scholar 

  81. 81.

    Moody JO, Robert VA, Connolly JD, Houghton PJ. Antiinflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of Sphenocentrum jollyanum Pierre (Menispermaceae). J Ethnopharm. 2006;104:87–91.

    CAS  Article  Google Scholar 

  82. 82.

    Moundipa PF, Njayou FN, Yantitoum S, Sonke B, Tchouanguep FM. Medical plants used in the Bamoun region of the western province of Cameroon against jaundice and other liver disorders. Cam J Biol Biochem Sci. 2002;12:39–46.

    Google Scholar 

  83. 83.

    Njayou FN, Ngoungoure FP, Tchana A, Moundipa PF. Protective Effect of Khaya grandifoliola C. DC. stem bark extract on carbon tetrachloride- induced hepatotoxicity in rats. Inter J Indige Med Plants. 2013;29:11–6.

    Google Scholar 

  84. 84.

    Njayou FN, Aboudi ECE, Tandjang MK, Tchana AK, Ngadjui BT, Moundipa PF. Hepatoprotective and antioxidant activities of stem bark extract of Khaya grandifoliola (Welw) CDC and Entada africana Guill. et Perr. J Nat Prod. 2013;6:73–80.

    Google Scholar 

  85. 85.

    Green EC. The WHO forum on traditional medicine in health systems, Harare, Zimbabwe, February 14–18, 2000. J Altern Complement Med. 2000;6:379–82.

    CAS  PubMed  Article  Google Scholar 

  86. 86.

    Dadzeasah PEA. Safety Evaluation and Hepatoprotective Activity of the Aqueous Stem Bark Extract of Spathodea Campanul at a. Kumasi: A Thesis Submitted Kwame Nkrumah University of Science and Technology; 2012.

    Google Scholar 

  87. 87.

    Gormann R, Schreiber L, Kolodziej H. Cuticular wax profiles of leaves of some traditionally used African Bignoniaceae. Z Naturforsch C. 2004;59:631–5.

    CAS  PubMed  Article  Google Scholar 

  88. 88.

    Mbosso EJ, Ngouela S, Nguedia JC, Penlap V, Rohmer M, Tsamo E. Spathoside, a cerebroside and other antibacterial constituents of the stem bark of Spathodea campanulata. Nat Prod Res. 2008;22:296–304.

    CAS  PubMed  Article  Google Scholar 

  89. 89.

    Amole O, Izegbu C, Onakoya A, Dada O. Toxicity studies of the aqueous extract of Vernonia amygdalina. Biomed Res. 2006;17:39–40.

    Google Scholar 

  90. 90.

    Orji OU, Ibiam UA, Aja PM, Uraku AJ, Inya-Agha OR, Ugwu Okechukwu PC. Hepatoprotective activity of ethanol extract of Vernonia ambigua against carbon tetrachloride induced hepatotoxicity in albino rats. IOSR J Dental Med Sci. 2015;14:22–9.

    Google Scholar 

  91. 91.

    Mandlekar S, Kong AN. Mechanisms of famoxiten Apoptoses. Apoptosis. 2001;6:469–77.

    CAS  PubMed  Article  Google Scholar 

  92. 92.

    Ekpo A, Eseyin A, Ikpeme O, Edoho J. Studies on some biochemical effects of Vernonia amygdalina in rats. J Biochem. 2007;2:193–7.

    Google Scholar 

  93. 93.

    Leonard S, Karen L, Bruce B, Thomas K, Jay H. Complementary and Alternative medicine in chronic liver disease. Hepatology. 2002;34:595–603.

    Google Scholar 

  94. 94.

    Aluko BT, Oloyede OI, Afolayan AJ. Hepatoprotective activity of Ocimum americanum L Leaves against paracetamol – induced liver damage in rats. Amer J Life Sci. 2013;1:37–42.

    Article  Google Scholar 

  95. 95.

    Oboh G. Antioxidative potential of Ocimum gratissimum and Ocimum canum leaf polyphenols and protective effects on some pro-oxidants induced lipid peroxidationin rat brain: An in vitro study. American J Food Technol. 2008;3:325–34.

    CAS  Article  Google Scholar 

  96. 96.

    Olorunnisola OS, Akintola AO, Afolayan AJ. Hepatoprotective and antioxidant effect of Sphenocentrum jollyanum (Menispermaceae) stem bark extract against CCl4– induced oxidative stress in rats. Afri J Pharm Pharmacol. 2011;5:1241–6.

    Article  Google Scholar 

  97. 97.

    Olorunnisola OS, Bradley G, Afolayan AJ. Protective Effect of Tulbaghia violacea Harv. on Aortic P at hology, Tissue Antioxidant Enzymes and Liver Damage in Diet-Induced at herosclerotic Rats. Int J Mol Sci. 2012;13:12747–60.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  98. 98.

    Olorunnisola OS, Bradley G, Afolayan AF. Antioxidant properties and cytotoxicity Evaluation of methanolic extract of dried and fresh rhizomes of Tulbaghia violacea. Afr J Pharm Pharmacol. 2011;5:2490–7.

    Google Scholar 

  99. 99.

    Donfack JH, Simob CFF, Ngamenic B, Tchanaa AN, Kerrd PG, Finzie PV, et al. Antihepatotoxic and antioxidant activities of methanol extract and isolated compounds from Ficus chlamydocarpa. Nat prod Comm. 2010;5:1607–12.

    CAS  Google Scholar 

  100. 100.

    Gbadegesin MA, Adegoke AM, Ewere EG, Odunola OA. Hepatoprotective and anticlastogenic effects of ethanol extract of Irvingia gabonensis (IG) leaves in sodium arsenite-induced toxicity in male Wistar rats. Niger J Physiol Sci. 2014;2:029–36.

    Google Scholar 

  101. 101.

    Souza SMC, Aquino LCM, Bandeira MAM, Nobre MEP, Viana GSB. Antiinflammatory and antiulcer properties of tannins from Myracrodruon urundeuva Allemão (Anacardiaceae) in Rodents. Phytother Res. 2007;21:220–5.

    CAS  PubMed  Article  Google Scholar 

  102. 102.

    Abdallah HM, Ezzat SM, El Dine RS, Abdel-Sattar E, Abdel-Naim AB. Protective effect of Echinops galalensis against CCl4 − induced injury on the human hepatoma cell line (Huh7). Phytochem Lett. 2013;6:73–8.

    CAS  Article  Google Scholar 

  103. 103.

    Muthumani P, Meera R, Sundaraganapathy DP, Mohamed Sheik AS, Cholarja K. Biological Evaluation of dried fruits of Lawsonia inermis. J Pharm Biomed Sci. 2010;1:1–5.

    Google Scholar 

  104. 104.

    Donkor SC, Quainoo AK, Gustav M. Propagation of Henna (Lawsonia inermis) cuttings using nathelene acetic acid, indole-3– butyric acid and wood ash. JPBAS. 2013;1:115–23.

    Google Scholar 

  105. 105.

    Abdulmoneim SM. Evaluation of Lawsonia inermis Linn. (Sudanese Henna) leaf extracts as an antimicrobial agent. Res J Biol Sci. 2007;2:419–23.

    Google Scholar 

  106. 106.

    Mohamed MA, Taj Eldin IM, Mohammed AH, Hassan HM. Effects of Lawsonia inermis L. (Henna) leaves’ methanolic extract on carbon tetrachloride-induced hepatotoxicity in rats. J Intercult Ethnopharmacol. 2016;5:22–6.

    PubMed  Article  Google Scholar 

  107. 107.

    Sanni S, Thilza IB, Ahmed MT, Sanni FS, Talle M, Okwor GO. The effect of aqueous leaves extract of henna (Lawsonia inermis) in carbon tetrachloride induced hep at o-toxicity in swiss albino mice. Academia arena. 2010;2:87–9.

    Google Scholar 

  108. 108.

    Uma D, Aida W. Optimiz at ion of extraction parameters of total phenolic compounds from Henna (Lawsonia inermis) leaves. Sains Malays. 2010;39:119–28.

    CAS  Google Scholar 

  109. 109.

    Kuete V, Ngameni B, Fotso-Simo CC, Kengap TR, Tchaleu NB, Meyer JJM, et al. Antimicrobial activity of the crude extracts and compounds from Ficus chlamydocarpa and Ficus cordata (Moraceae). J Ethnopharmacol. 2008;120:17–24.

    CAS  PubMed  Article  Google Scholar 

  110. 110.

    Bamps P. Guttiferae. (Clusiaceae). In: Boutique R, editor. Flore du Congo belge et du Ruanda-Burundi, Spermaphytes, Jardin Botanique National de Belgique, Brussels, Belgium. 1970. p. p 40.

    Google Scholar 

  111. 111.

    Vouffo EY, Donfack FM, Temdie RJ, Ngueguim FT, Donfack JH, Dzeufiet DS, et al. Hepatho-nephroprotective and antioxidant effect of stem bark of Allanblackia gabonensis aqueous extract against acetaminophen-induced liver and kidney disorders in rats. Journal of Experimental and Integrative Medicine 2012. 2012;2(4):337–44.

    Google Scholar 

  112. 112.

    Azebaze AG, Ouahouo BM, Vardamides JC, Valentin A, Kuete V, Acebey L, et al. Antimicrobial and antileishmanial xanthones from the stem bark of Allanblackia gabonensis (Guttiferae). Nat Product Res. 2008;22:333–41.

    CAS  Article  Google Scholar 

  113. 113.

    Ymele VE, Dongmo AB, Dimo T. Analgesic and anti-inflammatory effect of the aqueous extract of the stem bark of Allanblackia gabonensis (guttiferae). Inflammopharmacology. 2011. doi:10.1007/s10787–011–0096–2.

    PubMed  Google Scholar 

  114. 114.

    Ijeh II, Ukweni AI. Acute effect of administrationof ethanol extracts of Ficus exasperate vahl on kidney function in albino wistar rats. J Med Plant Res. 2007;1:027–9.

    Google Scholar 

  115. 115.

    Odutuga AA, Dairo JO, Ukpanukpong RU, Eze FN. Hepatoprotective activity of ethanol extracts of Ficus exasperat a leaves on acetaminophen-induced hep at otoxic rats. Merit Res J Biochem Bioinform. 2014;2:028–33.

    Google Scholar 

  116. 116.

    Njayou NI, Moundipa PF, Donfack JH, Chuisseu PD, Tchana AN, Ngadjui BT, et al. Hepato-protective, antioxidant activities and acute toxicity of a stem bark extract of Erythrina senegalensis DC. Int J Biol Chem Sci. 2010;3:738–47.

    Google Scholar 

  117. 117.

    Njayou FN, Moundipa PF, Tchana AN, Ngadjui BT, Tchouanguep FM. Inhibition of microsomal lipid peroxidationand protein oxidation by extracts from plants used in Bamun folk medicine (Cameroon) Against Hep at itis. Afr J Trad Compl Altern Med. 2008;5:278–89.

    CAS  Google Scholar 

  118. 118.

    Virtanen P, Lassila V, Soderstrom KO. Protoberberine alkaloids from Enantia chlorantha therapy of allyl-alcohol and D-galactosamine traumatized rats. Pathobiology. 1993;61(1):51–6.

    CAS  PubMed  Article  Google Scholar 

  119. 119.

    Sanogo R, Germano MP, D’Angelo V, Gugliemo M, De Pascuale R. Antihep at otoxic properties of Entada africana (Mimosaceae). Phytother Res. 1998;12:157–9.

    Article  Google Scholar 

  120. 120.

    Ruby AJ, Kuttan G, Dinesh BK, Rajasekharan KN, Kuttan R. Anti-tumor and antioxidant activity of natural curcuminoids. Cancer Lett. 1995;94:79–83.

    CAS  PubMed  Article  Google Scholar 

  121. 121.

    Martinez GM, Giuliani A, Leon-F OS, Davison GP, Nunez-Selles AJ. Effect of Mangifera indica L. extract (QF808) on protein and hepatic microsome peroxidation. Phytother Res. 2001;15:581–5.

    CAS  PubMed  Article  Google Scholar 

  122. 122.

    Sanchez GM, Re L, Giuliani A, Nunez-Selles AJ, Davison GP, Leon FOS. Protective effects of Mangifera indica L. extract, Mangiferin, and selected antioxidants against TPA- induced biomolecule oxidation and peritoneal macrophage activ at ion in mice. Pharmacol Res. 2000;42:5665–73.

    Article  Google Scholar 

  123. 123.

    Middleton MJR, Chithan K, Theoharis CT. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease and cancer. Pharmacol Rev. 2000;52:673–751.

    CAS  PubMed  Google Scholar 

  124. 124.

    Noguchi N, Komuro E, Niki E, Willson RL. Action of curcumin as an antioxidant against lipid peroxidation. Yukagaku. 1994;43:1045–51.

    CAS  Google Scholar 

  125. 125.

    Wandji J, Fomum TZ, Tillequin F, Seguin E, Koch M. Two isoflavones from Erythrina senegalensis. Phytochemistry. 1994;35:245–8.

    CAS  Article  Google Scholar 

  126. 126.

    Mulata HN, Daniel S, Melaku U, Ergete W, Gnanasekaran N. Protective Effects of Calpurnia aurea Seed Extract on HAART Hepatotoxicity. Eur J Med Plants. 2015;9:1–12.

    Article  Google Scholar 

  127. 127.

    Iroanya O, Okpuzor J, Adebesin O. Hepatoprotective and antioxidant properties of a triherbal formulation against carbon tetrachloride induced hepatotoxicity. IOSR J Pharm. 2012;2:130–6.

    Google Scholar 

  128. 128.

    Atawodi SE. In vivo antioxidant, organ protective, ameliorative and cholesterol lowering potential of ethanolic and methanolic extracts of “Ata-Ofa” polyherbal tea (A-Polyherbal). Int J Res Pharm Sci. 2011;2:473–82.

    Google Scholar 

  129. 129.

    Omalu ICJ, Egwim EC, Mgbemena CC, Eke SS, Ubanwa D, Busari MB, et al. Free Radical Scavenging Activity of the Nigerian Leech (Aliolimn at is michaelseni) Saliva Extract. Bri J Pharm Res. 2015;8:1–6.

    Article  Google Scholar 

  130. 130.

    Omalu ICJ, Egwim EC, Abdulraman K, Ibrahim S, Hassan C, Eke SS. Free Radical Scavenging Activity and Protein Concentration and Profile of the Blowfly Maggot (Lucilia robineau) Excretion/Saliva Extract. Bri J Pharm Res. 2016;9:1–6.

    Article  Google Scholar 

  131. 131.

    Shittu OK, Lawal B, Oluyomi OI. Effects of Methanol Extract of Musca domestica Larvae on Antioxidants Enzymes in T. Brucei Infected Rats. Nig J Biochem Mol Biol. 2014;29(2):1–10.

    Google Scholar 

  132. 132.

    Shittu OK, Lawal B, Haruna GM, Berinyuy EB, Yusuf AA, Ibrahim AM. Hepato-Curative Effects of Methanol extract from Nigeria Bee Propolis in Carbon Tetrachloride (CCL4) Intoxicated Rat. Euro J Biotechnol Biosci. 2015;3:12–6.

    Google Scholar 

  133. 133.

    Muruke MH. Assessment of antioxidant properties of honeys from Tanzania. J Biol Agric Health. 2014;4:22–32.

    Google Scholar 

  134. 134.

    Adeniyi KA, Olayemi IK, Shittu KO, Busari MB, Mohammed SS, Bashir L, et al. Comparative phytochemical and antinutritional constituents of Nigeria sweet and bitter honey varieties. World J Pharm Res. 2016;5:255–67.

    Google Scholar 

  135. 135.

    Nyanzi R, Shuping DSS, Jooste PJ, Eloff JN. Antibacterial and Antioxidant Activity of Extracts from Selected Probiotic Bacteria. J Food Res. 2015;4(5):122–32.

    Article  Google Scholar 

  136. 136.

    Olutayo O, Doyinsola I, Simon O, Abayomi O, Thomas S. Phytochemical and antioxidant properties of some Nigerian medicinal plants. Am J Sci Ind Res. 2011;4:328–32.

    Google Scholar 

  137. 137.

    Okoh SO, Asekun OT, Familoni OB, Afolayan AJ. Composition and Antioxidant Activities of leaf and root volatile Oils of Morinda lucida. Nat prod Comm. 2011;6(10):1537–41.

    CAS  Google Scholar 

  138. 138.

    Amoussa AO, Sanni A, Lagnika L. Antioxidant activity and total phenolic, flavonoid and flavonol contents of the bark extracts of Acacia ataxacantha. J Pharmacog Phytochem. 2015;4:172–8.

    Google Scholar 

  139. 139.

    Sawadogo WR, Maciuk A, Banzouzi JT, Champy P, Figadere B, Guissou IP, et al. Mutagenic effect, antioxidant and anticancer activities of six medicinal plants from Burkina Faso. Nat Prod Res. 2011. doi:10.1080/14786419.2010.534737.

    PubMed  Google Scholar 

  140. 140.

    Tauseef S, Ali MS, Ahmed A, Ali MI, Ahmed Z, Sherwani SK, et al. In vitro Antioxidant activity analysis of five medicinally important plants. J Pharmacog Phytochem. 2013;2:183–8.

    Google Scholar 

  141. 141.

    Lagnika L, Anago E, Sanni A. Screening for antibacterial, antioxidant activity and toxicity of some medicinal plants used in Benin folkloric medicine. Journal of Medicinal Plants Research Vol. 2011;5(5):773–7.

    Google Scholar 

  142. 142.

    Kabine O, Mamadou BS, Fatoumata B, Namagan K, Luopou HN, Mamadou BA. Anti-oxidative activity of fruit extracts of some medicinal plants used against chronic diseases (diabetes, hypertension) in Kankan, Guinea. J Plant Sci. 2015;3:1–5.

    Google Scholar 

  143. 143.

    Oloyede GK, Onocha PA, Soyinka J, Oguntokun OW, Thonda E. Phytochemical screening, antimicrobial and antioxidant activities of four Nigerian medicinal plants. Annals Biol Res. 2010;1:114–20.

    Google Scholar 

  144. 144.

    Lamien-Meda A, Lamien CE, Compaore MMY, Meda RNT, Kiendrebeogo M, et al. Polyphenol content and antioxidant activity of fourteen wild edible fruits from Burkina Faso. Molecules. 2008;13:581–94.

    CAS  PubMed  Article  Google Scholar 

  145. 145.

    Kazeem MI, Akanji MA, Hafizur RM, Choudhary MI. AntiglyCAT ion, antioxidant and toxicological potential of polyphenol extracts of alligator pepper, ginger and nutmeg from Nigeria. Asian Pac J Trop Biomed. 2012;2:727–32.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  146. 146.

    Aliyu AB, Musa AM, Ibrahim MA, Ibrahim H, Oyewale AO. Preliminary Phytochemical Screening and antioxidant activity of leave extract of Albizia Chevalieri Harms (Leguminoseae-Mimosoideae). Bayero J Pure Appl Sci. 2009;2:149–53.

    Google Scholar 

  147. 147.

    Osadebe PO, Festus BC, Philip FU, Nneka RN, Ijeoma EA, Nkemakonam CO. Phytochemical analysis, Hepatoprotective and antioxidant activity of Alchornea cordifolia Methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats. Asian Pac J Trop Med. 2012;5:289–93.

    PubMed  Article  Google Scholar 

  148. 148.

    Otunola GA, Afolayan AJ. Evaluation of the polyphenolic contents and some antioxidant properties of aqueous extracts of Garlic, Ginger, Cayenne Pepper and their mixture. J Appl Bota Food Qual. 2013;86:66–70.

    Google Scholar 

  149. 149.

    Chinaka ON, Julius OO, Motunrayo GA. In vitro antioxidant potentials of some herbal plants from Southern Nigeria. J Med Sci. 2013;13(1):56–61.

    Article  Google Scholar 

  150. 150.

    Adetutu A, Sinbad OO, Bukoye OE. Phytochemical composition, antioxidant properties and antibacterial activities of five West-African green leafy vegetables. Senra Acad Pub Brit Col. 2013;7:2357–62.

    CAS  Google Scholar 

  151. 151.

    Atawodi SE, Onaolapo GS. Comparat ive in vitro antioxidant potential of different parts of Ipomoea asarifolia, Roemer and Schultes, Guiera senegalensis. J F Gmel and Anisopus mannii N E Brown Braz J Pharma Sci. 2010;46:246–50.

    Google Scholar 

  152. 152.

    Salau AK, Yakubu MT, Oladiji AT. In vitro and In vivo Antioxidant Activity of Aqueous Extracts of Anogeissus l eiocarpus (DC) Guill and Perr and Terminalia avicennioides Guill and Perr Root Barks. Cam J Biol Bioche Sci. 2015;23:9–16.

    Google Scholar 

  153. 153.

    Karou SD, Tchacondo T, Ouattara L, Anani O, Savadogo A, Agbonon A, et al. Antimicrobial, antiplasmodial, haemolytic and antioxidant activities of crude extracts from three selected Togolese medicinal plants. Asian Pac J Trop Med. 2011;4:808–13.

    PubMed  Article  Google Scholar 

  154. 154.

    Aliyu AB, Ibrahim MA, Musa AM, Ibrahim H, Abdulkadir IE, Oyewale AO. Evaluation of antioxidant activity of leave extract of Bauhinia rufescens Lam. (Caesalpiniaceae). J Med Plants Res. 2009;3:563–7.

    Google Scholar 

  155. 155.

    Sawadogo WR, Meda A, Lamien CE, Kiendrebeogo M, Guissou IP, Nacoulma OD. Phenolic Content and Antioxidant Activity of Six Acanthaceae from Burkina Faso. J Biol Sci. 2006;6:249–52.

    CAS  Article  Google Scholar 

  156. 156.

    Aladesanmi AJ, Iwalewa EO, Adebayo AC, Akinkunmi EO, Taiwo BJ, Olorunmola FO, Lamikanra A. Antimicrobial and Antioxidant activities of some Nigerian Medicinal Plants. Afri J Trad CAM. 2007;4(2):173–84.

    Google Scholar 

  157. 157.

    Anago E, Lagnika L, Gbenou J, Loko F, Moudachirou M, Sanni A. Antibacterial Activity and Phytochemical Study of Six Medicinal Plants used in Benin. Pak J Biol Sci. 2011. doi:10.3923/pjbs.2011.

    PubMed  Google Scholar 

  158. 158.

    Emeka EJI, Fang-Fang L, Rong- Rong C, Yan L, Conrad AO, Ing-Jun Z. Anticancer and Free radical scavenging activity of some Nigerian food plants in vitro. Int J Cancer Res. 2015;11:41–51.

    Article  CAS  Google Scholar 

  159. 159.

    Falodun A, Irabor EEI. Phytochemical, Proximate, Antioxidant And Free Radical Scavenging Evaluations Of Calliandria Surinamensis. Acta Poloniae Pharma Drug Res. 2008;65:571–5.

    CAS  Google Scholar 

  160. 160.

    Egwim EC, Elem RC, Egwuche RU. Proximate composition, phytochemical screening and antioxidant activity of ten selected wild edible Nigerian mushrooms. Am J Food Nutr. 2011;1(2):89–94.

    Article  Google Scholar 

  161. 161.

    Aina DA, Jonathan SG, Olawuyi OJ, Ojelabi DO, Durowoju BM. Antioxidant, antimicrobial and phytochemical properties of alcoholic extracts of Cantharellus cibarius – a Nigerian mushroom. N Y Sci J. 2012;5:114–20.

    Google Scholar 

  162. 162.

    Ayoola GA, Coker HAB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res. 2008;7:1019–24.

    Google Scholar 

  163. 163.

    Madubuike GK, Onoja SO, Ezeja MI. Antioxidant and Hepatoprotective Activity of Methanolic Extract of Cassia sieberiana Leaves in Carbon Tetrachloride-induced Hepatotoxicity in Rats. J Adv Med Pharmaceut Sci. 2015;2(1):1–9.

    Article  Google Scholar 

  164. 164.

    Lagnika L, Amoussa O, Oketokoun SA, Adjovi Y, Sanni A. In vitro antifungal and antioxidant activities of two Benin medicinal plants. J Med Plants Res. 2014;8:513–9.

    CAS  Article  Google Scholar 

  165. 165.

    Konan Y, Witabouna KM, Bassirou B, Kagoyire K. Antioxidant activity and total phenolic content of nine plants from Côte d’Ivoire (West Africa). J Appl Pharm Sci. 2014;4:036–41.

    CAS  Google Scholar 

  166. 166.

    Karim T, Béourou S, Touré AO, Ouattara K, Meité S, Ako A, et al. Antioxidant activities and estimation of the phenols and flavonoids content in the extracts of medicinal plants used to treat malaria in Ivory Coast. Int J Curr Microbiol App Sci. 2015;4:862–74.

    Google Scholar 

  167. 167.

    Oghenerobo VI, Falodun A. Antioxidant Activities of the Leaf Extract and Fractions of Cola lepidota K. Schum (sterculiaceae). Nig J Biotech. 2013;25:31–6.

    Google Scholar 

  168. 168.

    Karou D, Mamoudou HD, Simpore J, Traore AS. Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso. Afr J Biotechnol. 2005;8:823–8.

    Google Scholar 

  169. 169.

    Tsado, N.A., Lawal, B. Ossa, P.C., Jagaba A., Kontagora, G.N., Gboke, J.A, et al. Antioxidants and Antimicrobial Activities of Methanol Extract of Newbouldia laevis and Crateva adansonii. J. Pharm Allied Health Sci. 2016b. DOI: 10.3923/jpahs.2016.

  170. 170.

    Kiendrebeogo M, Coulibaly AY, Nebie RCH, Zeba B, Lamien CE, Meda AL, et al. Antiacetylcholinesterase and antioxidant activity of essential oils from six medicinal plants from Burkina Faso. Braz J Pharmacog. 2011;21:63–9.

    CAS  Google Scholar 

  171. 171.

    Tibiri A, Sawadogo RW, Ouedraogo N, Banzouzi JT, Guissou IP, Nacoulma GO. Evaluation of antioxidant activity, total phenolic and flavonoid contents of Entada africana Guill. et Perr. (Mimosaceae) Organ Extracts. Res J Med Sci. 2010;4:81–7.

    Google Scholar 

  172. 172.

    Aliyu AB, Ibrahim MA, Ibrahim H, Musa AM, Lawal AY, Oshanimi JA, et al. Free radical scavenging and total antioxidant capacity of Methanol extract of Ethulia conyzoides growing in Nigeria. Rom Biotechnol Letters. 2012;17:7458–65.

    CAS  Google Scholar 

  173. 173.

    Ashafa AOT, Grierson DS, Afolayan AJ. In vitro Antioxidant Activity of Extracts from the Leaves of Felicia Muricata Thunb. An Underutilized Medicinal Plant in the Eastern Cape Province, South Africa. Afr J Tradit Compl Altern Med. 2010;7:296–302.

    CAS  Google Scholar 

  174. 174.

    Ojo OA, Akintayo CO. Assessment of antioxidant activity of Ficus asperifolia Miq aqueous extract - In vitro studies. J Phytopharmacol. 2014;3:16–21.

    Google Scholar 

  175. 175.

    Hamzah RU, Jigam AA, Makun HA, Egwim EC. Phytochemical Screening and In vitro Antioxidant Activity of Methanolic Extract Of Selected Nigerian Vegetables. Asian J Basis and Appl Sci. 2014;1:1–14.

    Google Scholar 

  176. 176.

    Chinedu OJ, Ibrahim S, Chukwuemeka NH, Uwadiegwu AA. Effects of fractionated methanolic leaf extract of Gongronema latifoliumon CCl4−induced Wistar Albino Rats. J Pharmacol Clin Res. 2015;1:551–5.

    Google Scholar 

  177. 177.

    Adetutu A, Owoade AO. Hepatoprotective and antioxidant effect of Hibiscus polyphenol rich extract (HPE) against carbon tetrachloride (CCL4) –induced damage in rats. B J Med Medical Res. 2013;3:1574–86.

    Article  Google Scholar 

  178. 178.

    Agyare C, Dwobeng AS, Agyepong N, Boakye YD, Mensah KB, Ayande PG, et al. Antimicrobial, antioxidant, and wound healing properties of Kigelia africana (Lam.) Beneth. and Strophanthus hispidus DC. Adv Pharmacol Sci. 2013. doi:10.1155/2013/692613.

    PubMed  PubMed Central  Google Scholar 

  179. 179.

    Okereke SC, Elekwa I. Studies on the In vitro Antioxidant Activity of Laportea Aestuans Leaf Extract. IOSR J Env Sci, Toxicol Food Technol. 2014;8:33–41.

    Article  CAS  Google Scholar 

  180. 180.

    Agbafor, K.N., Nwachukwu, N. (2011). Phytochemical analysis and antioxidant property of leaf extracts of Vitex doniana and Mucuna pruriens. Biochem. Res. Int. 2011, doi:10.1155/2011/459839

  181. 181.

    Badu M, Mensah JK, Boadi NO. Antioxidant Activity of methanol and ethanol/w ater extracts of Tetrapleura tetraptera and parkia biglobosa. Int J Pharm Bio Sci. 2012;3:312–21.

    Google Scholar 

  182. 182.

    Seidu KT, Osundahunsi OF, Olaleye MT, Oluwalana IB. Chemical Composition, Phytochemical Constituents and Antioxidant Potentials of Lima Bean Seeds Coat. Ann Food Sci Tech. 2014;15:288–98.

    Google Scholar 

  183. 183.

    Ouédraogo N, Tibiri A, Sawadogo RW, Lompo M, Hay AE, Koudou J, et al. Antioxidant anti-inflammatory and analgesic activities of aqueous extract From stem bark of Pterocarpus erinaceus Poir. (Fabaceae). J Med Plants Res. 2011;5:2047–53.

    Google Scholar 

  184. 184.

    Yougbaré-Ziébrou MN, Ouédraogo N, Lompo M, Bationo H, Yaro B, Gnoula C, et al. Anti-inflammatory, analgesic and antioxidant activities of an aqueous extract of Saba senegalensis Pichon stems with leaves (Apocynaceae). Phytothérapie. 2015. doi:10.1007/s10298–015–0992–5.

    Google Scholar 

  185. 185.

    Tsado NA, Lawal B, Kontagora GN, Muhammad BM, Yahaya MA, Gboke JA, et al. Antioxidants and Antimicrobial- Activities of Methanol Leaf Extract of Senna occidentalis. J Adv Med Pharma Sci. 2016;8(2):1–7.

    Article  Google Scholar 

  186. 186.

    Adu OB, Folorunso OS, Adeola SA, Godfrey C. Antioxidant and Antimicrobial properties of volatile oil of Terminalia catappa and its inhibition on the extracellular protease of Pseudomonas aeruginosa. Res J Life Sci. 2013;1:1–8.

    Google Scholar 

  187. 187.

    Adesanoye OA, Farombi EO. In vitro Antioxidant Properties of methanolic leaf extract of Vernonia amygdalina Del. Niger. J Physiol Sci. 2014;29:091–101.

    CAS  Google Scholar 

  188. 188.

    Elnour MA, Elegamy AA, Koko WS, Khalid A, Fadul E. Antioxidant Activity and Cytotoxicity of some Sudanese Medicinal Plants. Inter J Adv Indust Eng. 2013;1:20–3.

    Google Scholar 

  189. 189.

    Hala AAM. Quality of medicinal plants traditionally used in Sudan as affected by ionizing radi at ion treatments. Khartoum: A Phd thesis submitted to Faculty of Agriculture University of Khartoum; 2009.

    Google Scholar 

  190. 190.

    Bakr RO, Omer EA, Abdel-Razik KA, Abuelnaga ASM, Danial EN, Elgindy AG. Antioxidant and anti-listerial activities of selected Egyptian medicinal plants. Afr J Microbiol Res. 2013;7:459 0–95.

    Article  Google Scholar 

  191. 191.

    Kabbashi AS, Koko WS, Mohammed EE, Musa AN, Osman EE, Dahab MM. In vitro amoebicidal, antimicrobial and antioxidant activities of the plants Adansonia digitata and Cucurbit maxima. Adv Med Plant Res. 2014;2:50–7.

    Google Scholar 

  192. 192.

    Ali SK, Hamed AR, Soltan MM, Hegazy UM, Elgorashi EE, El-Garf IA, et al. In-vitro evaluation of selected Egyptian traditional herbal medicines for treatment of Alzheimer disease. BMC Complement Altern Med. 2013;13:121.

    PubMed  PubMed Central  Article  Google Scholar 

  193. 193.

    Soumia K, Tahar D, Lynda L, Saida B, Chabane C, Hafidha M. Antioxidant and antimicrobial activities of selected medicinal plants from Algeria. J Coast Life Med. 2014;2:478–83.

    Google Scholar 

  194. 194.

    Elmestiri FM. Evaluation of selected Libyan medicinal plant extracts for their antioxidant and anticholinesterase activities. In: Thesis submitted for the degree of Doctor of Philosophy. Newcastle upon Tyne: School of Biology and Psychology University of Newcastle upon Tyne; 2011. NE 1 7RU.

    Google Scholar 

  195. 195.

    Ezejiofor AN, Udowelle NA, Orisakwe OE. Nephroprotective and antioxidant effect of aqueous leaf extract of Costus Afer Ker gawl on cyclosporin-a (Csa) induced nephrotoxicity. Clinical Phytoscience. 2016;2:11.

    Article  Google Scholar 

  196. 196.

    Hamid SY, Elegami AA, Koko WS, Abdelwahab SI, Bostman A. Anticancer and Antioxidant Activities of Three Sudanese Medicinal Plants Belong To The Family Combretaceae. Asian Acad Res J Multidiscipl. 2014;1:281–9.

    Google Scholar 

  197. 197.

    Elhaj AM, Osman EE, Koko WS, Garbi MI, Kabbashi AS. Antioxidant activity, phytochemical screening and cytotoxicity of ethanolic leaves extract of Antigonon leptopus. Res J Agric Environm Manage. 2015;4:202–7.

    Google Scholar 

  198. 198.

    Sami A, Abdulmottaleb Z, Mohamed S, Rmeli N, Fatah GA. Antioxidant Activity of Eight Libyan Medicinal Plants Extracted Using the Microwave Technique.Book of abstract.The 3rd International symposium on medicinal plants, their cultivationand aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  199. 199.

    Guendouze-Bouchefa N, Madani K, Chibane M, Boulekbache-Makhlouf L, Hauchard D, Kiendrebeogo E, et al. Phenolic compounds, antioxidant and antibacterial activities of three Ericaceae from Algeria. Ind Crop Prod. 2015;70(2015):459–66.

    CAS  Article  Google Scholar 

  200. 200.

    Abushama MF, Hilmi YI, AbdAlgadir HM, Fadul E, Khalid HE. Lethality and antioxidant activity of some Sudanese medicinal plants’ fixed oils. Eur J Med Plants. 2014;5:563–70.

    Article  Google Scholar 

  201. 201.

    Rashed K, Guder A. Comparative Study of Antioxidant Activity from some Egyptian Plants and Phytochemical Composition. Res J Med Plant. 2015;4:146–59.

    Article  Google Scholar 

  202. 202.

    Nassr-Allah AA, Aboul-Enein AM, Aboul-Enein KM, Lightfoot DA, Cocchetto A, El-Shemy HA. Anti-cancer and anti-oxidant activity of some Egyptian medicinal plants. J Med Plants Res. 2009;3:799–808.

    CAS  Google Scholar 

  203. 203.

    Garbi MI, Kabbashi AS, Osman EE, Dahab MM, Koko WS, Ahmed IF. Antioxidant activity and phytochemical screening of the methanolic leaves extract of B. Rufescens (LAM). Inter J Biol Pharma, Res. 2015;6:930–4.

    Google Scholar 

  204. 204.

    Mariem BJ, Ghaith H, Maurizio B, Elyes KM. Antioxidant Activities in Calycotome villosa L. (Fabacées) from National Park of Bou Kornine, Tunisia. Book of abstract.The 3rd International symposium on medicinal plants, their cultivationand aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  205. 205.

    Kaabour F, Belambri SA, Bouriche H, Senator A. Antioxidant Activity of Aqueous Tea Extracts. Book of abstract.The 3rd International symposium on medicinal plants, their cultivation and aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  206. 206.

    Dudonn SE, Vitrac X, Coutiere P, Woillez M, Merillon JM. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC Assays. J Agric Food Chem. 2009;57:1768–74.

    Article  CAS  Google Scholar 

  207. 207.

    Oumar YS, Nathalie GK, Souleymane M, Karamoko O, Alexis BG, David GJ, et al. In vitro antioxidant activity of extracts of the root Cochlospermum planchonii Hook. f. ex. Planch (Cochlospermaceae). J Pharmacog Phytochemist. 2014;3(4):164–70.

    Google Scholar 

  208. 208.

    Osman ME, Yassen HH, Deng JD, Mustafa RO, Hussein SM. General Phytochemical Screening and Antioxidant Activity of Some Sudanese Medicinal Plants. J forest produ Ind. 2014;3:292–5.

    Google Scholar 

  209. 209.

    Alhadi EA, Khalid HS, Alhassan MS, Ali AA, Babiker SG, Alabdeen EMZ, et al. Antioxidant and cytotoxicity activity of Cordia Africana in Sudan. Adv Med Plant Res. 2015;3:29–32.

    Google Scholar 

  210. 210.

    Mohamed B, Amar D, Zohra M, Hadda G, Mohamed Y. Antioxidant activity of phenolic extracts from some local pumpkin seeds (Cucurbita pepo). Book of abstract.The 3rd International symposium on medicinal plants, their cultivationand aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  211. 211.

    Alghazeer R, El-Saltani H, Saleh NA, Al-Najjar A, Mahboba B, Hebail NF, et al. Antioxidant and antimicrobial activities of Cynara scolymus L. Rhizomes. Modern Appl Sci. 2012;6:54–63.

    CAS  Article  Google Scholar 

  212. 212.

    Hanane B, Lilia B, Khodir M, Mohamed K. Eucalyptus globulus: Polyphenols Extraction and Antioxidant Effect. Book of abstract.The 3rd International symposium on medicinal plants, their cultivationand aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  213. 213.

    Alghazeer R, El-Saltani H, Saleh N, Al-Najjar A, Hebail F. Antioxidant and antimicrobial properties of five medicinal Libyan plants extracts. Nat Sci. 2012;4:324–35.

    CAS  Google Scholar 

  214. 214.

    Yasmina L, Sebaihi S, Remila SM, Chaher N, Debache N, Ayouni K, et al. Antioxidant Activity of Extracts of Fraxinus angustifolia. Book of abstract.The 3rd International symposium on medicinal plants, their cultivation and aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  215. 215.

    Derrafa I, Benchikh F, Amira S, Khennouf S, Dahamna S. Antioxidant and antiulcerogenic effects of Globularia alypum L. Extracts. Book of abstract.The 3rd International symposium on medicinal plants, their cultivation and aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  216. 216.

    Atef C, Anouar F, El-Hadda A, Azzedine C. Phytochemicals study, antioxidant and antimicrobial activities of Helianthemum lippii (L.) pers. in different stages of growth (somatic, flowering and fruiting). World J Pharm Pharm Sci. 2015;4:338–49.

    Google Scholar 

  217. 217.

    Kraouche NN, Kharfallah Y, Bejou F. Antioxidant Activity of Essential Oils Isolated from Selected Algerian Medicinal Plants. Book of abstract.The 3rd International symposium on medicinal plants, their cultivation and aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  218. 218.

    El Diwani G, El Rafie SH, Hawash S. Antioxidant activity of extracts obtained from residues of nodes leaves stem and root of Egyptian Jatropha curcas. African J Pharm Pharmacol. 2009;3:521–30.

    Google Scholar 

  219. 219.

    Fatiha B, Didier H, Naima G, Khodir M, Martin K, Léocadie K, et al. Phenolic composition, in vitro antioxidant effects and tyrosinase inhibitory activity of three Algerian Mentha species: M. spicata (L.), M. pulegium (L.) and M. rotundifolia (L.) Huds (Lamiaceae). Ind. Crops Prod. 2015;74:722–30.

    Article  CAS  Google Scholar 

  220. 220.

    Seddik K, Saliha D, Dalila B, Saliha D, Noureddine C, Abderahmane B, et al. Polyphenols and Antioxidant Properties of Extracts from Menthapulegium L. and Matricaria camomilla L. Book of abstract.The 3rd International symposium on medicinal plants, their cultivationand aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  221. 221.

    Belmimoun A, Meddah B, Meddah AT, Sonnet P. Antibacterial and antioxidant activities of the essential oils and phenolic extracts of Myrtus communis and Zygophylum album from Algeria. J Fundam Appl Sci. 2016;8(2):510–24.

    Article  Google Scholar 

  222. 222.

    Kabbashi AS, Eltayeb OM, Abuzeid NM, Garbi MI, Koko WS. Antioxidant activity and Cytotoxicity of Ethanolic Seeds Extract of Nigella sativa (Linn) in Sudan. Merit Res J Agric Sci Soil Sci. 2015;3:1–10.

    Google Scholar 

  223. 223.

    Djarmouni S, Abderrahmane B, Naouel B, Hayet T, Sabah B, Farida B, Ameni D, Fatima Z, Lekhmici A. Studies of Anti Bacterial and Antioxidant Potential of Methanol Extract/Fractions of Peganum harmala. Book of abstract.The 3rd International symposium on medicinal plants, their cultivationand aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  224. 224.

    Saliha D, Abir R, Mounira M, Dalila B, Daoud H, Assia B, Soulef B, Karima Y, Khadidja D. Antioxidant Activity of the Aqueous and Methanolic Extracts from Peganum harmala Seeds. Book of abstract.The 3rd International symposium on medicinal plants, their cultivationand aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  225. 225.

    Sifi I, Dzoyem JP, Ouinten M, Yousfi M, McGaw LJ, Eloff JN. Antimycobacterial, antioxidant and cytotoxic activities of essential oil of gall of pistacia atlantica desf. from Algeria. Afr J Tradit Complement Altern Med. 2015;12(3):150–5.

    Article  Google Scholar 

  226. 226.

    Saliha D, Seddik K, Djamila A, Abdrrahmane B, Lekhmici A, Noureddine C. Antioxidant Proprieties of Pistacia lentiscus l. Leaves Extracts. Book of abstract.The 3rd International symposium on medicinal plants, their cultivation and aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  227. 227.

    Nadia Z, Rachid M. Antioxidant Activity of Flavonoids Isolated from Rosmarinus officinalis L. J Plant Sci Res. 2016;3(1):142.

    Google Scholar 

  228. 228.

    Ameni D, Baghiani A, Djidel S, Boumerfeg S, Khennouf S, Arrar L. The Antioxidant and Lipid Peroxidation Activities of Rubus idaeus L. Extracts. Book of abstract.The 3rd International symposium on medicinal plants, their cultivation and aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  229. 229.

    Hamama B, Soumia B, Abderrahmane S. Total Polyphenolic Contents and Antioxidant Activity of Santolina chamaecyparissus. Book of abstract.The 3rd International symposium on medicinal plants, their cultivation and aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  230. 230.

    Giweli A, Džamić AM, Soković M, Mihailo S, Ristić Petar DM. Antimicrobial and antioxidant activities of essential oils of Satureja thymbra Growing Wild in Libya. Molecules. 2012;17:4836–50. doi:10.3390/molecules17054836.

    CAS  PubMed  Article  Google Scholar 

  231. 231.

    Sabah A, Fatiha B. Antioxidant Activity of Thapsia garganica Leaves and Roots Extracts. Book of abstract.The 3rd International symposium on medicinal plants, their cultivation and aspects of uses. Jordan: Beitzaman hotel and resort Petra; 2012.

    Google Scholar 

  232. 232.

    El-Shora HM, El-Amier YA, Awad MH. Antioxidant Activity of Leaf Extracts from Zygophyllum coccineum L. Collected from Desert and Coastal Habitats of Egypt. Int J Curr Microbiol App Sci. 2016;5(4):635–41.

    Article  Google Scholar 

  233. 233.

    Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complement Altern Med. 2008;8:54.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  234. 234.

    Adewusi EA, Steenkamp V. In vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from southern Africa. Asian Pac J Trop Med. 2011;11:60203–4.

    Google Scholar 

  235. 235.

    Wintola OA, Afolayan AJ. Phytochemical constituents and antioxidant activities of the whole leaf extract of Aloe ferox Mill. Pharmacog Mag. 2011;7:325–33.

    CAS  Article  Google Scholar 

  236. 236.

    Ahmed AS, Elgorashi EE, Moodley N, McGaw LJ, Naidoo V, Eloff JN. The antimicrobial, antioxidative, anti-inflammatory activity and cytotoxicity of different fractions of four South African Bauhinia species used traditionally to treat diarrhea. J Ethnopharmacol. 2012;143:826–39.

    PubMed  Article  Google Scholar 

  237. 237.

    Adamu M, Naidoo V, Eloff JN. The antibacterial activity, antioxidant activity and selectivity index of leaf extracts of thirteen South African tree species used in ethnoveterinary medicine to treat helminth infections. BMC Vet Res. 2014;10:52.

    PubMed  PubMed Central  Article  Google Scholar 

  238. 238.

    Yusuf L, Oladunmoye MK, Ogundare AO, Akinyosoye FA, Daudu OAY, Hassan GA. Antimicrobial and antioxidant properties of mistletoe (viscum album) growing on cola (cola nitida) tree in Akure North, Nigeria. J MicrobiolRes Rev. 2013;1(3):35–41.

    Google Scholar 

  239. 239.

    Olaokun OO, McGaw LJ, Eloff Jacobus JN, Naidoo V. Evaluation of the inhibition of carbohydrate hydrolysing enzymes, antioxidant activity and polyphenolic content of extracts of ten African Ficus species (Moraceae) used traditionally to treat diabetes. BMC Complement Altern Med. 2013;13:94.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  240. 240.

    Adebayo SA, Dzoyem JP, Shai LJ, Eloff JN. The anti-inflammatory and antioxidant activity of 25 plant species used traditionally to treat pain in southern African. BMC Complement Altern Med. 2015;15:159. doi:10.1186/s12906-015-0669-5.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  241. 241.

    Katerere DR, Eloff JN. Antibacterial and Antioxidant Activity of Sutherlandia frutescens (Fabaceae), A Reputed Anti-HIV/AIDS Phytomedicine. Phytother Res. 2005;19:779–81.

    PubMed  Article  Google Scholar 

  242. 242.

    Mokgotho, M.P., Gololo, S.C., Masoko, P., Mdee, L.K., Mbazima, V., Shai, L.J. et al., (2013). Isolation and Chemical Structural Characterisation of a Compound with Antioxidant Activity from the Roots of Senna italic. Evidence-Based Complementary and Alternative Medicine, 2013,

  243. 243.

    Komape NPM, Aderogba M, Bagla VP, Masoko PM, Eloff JN. Anti-bacterial and anti-oxidant activities of leaf extracts of Combretum vendee (combretecacea) and the isolation of an anti-bacterial compound. Afr J Tradit Complement Altern Med. 2014;11(5):73–7.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  244. 244.

    Naidoo V, Chikoto H, Bekker LC, Eloff JN. Antioxidant compounds in Rhoicissus tridentata extracts may explain their antibabesial activity. S Afr J Sci. 2006;102:198–200.

    CAS  Google Scholar 

  245. 245.

    Dzoyem JP, McGaw LJ, Eloff JN. In vitro antibacterial, antioxidant and cytotoxic activity of acetone leaf extracts of nine under-investigated Fabaceae tree species leads to potentially useful extracts in animal health and productivity. BMC Compl Alter Med. 2014;14:147.

    Article  CAS  Google Scholar 

  246. 246.

    Ahmed AS, McGaw LJ, Eloff JN. Evaluation of pharmacological activities, cytotoxicity and phenolic composition of four Maytenus species used in southern African traditional medicine to treat intestinal infections and diarrhoeal diseases. BMC Complem Alter Med. 2013;13:100.

    Article  CAS  Google Scholar 

  247. 247.

    Awouafack MD, Tane P, Eloff JN. Two new antioxidant flavones from the twigs of Eriosema robustum (Fabaceae). Phytochem Lett. 2013;6(2013):62–6.

    CAS  Article  Google Scholar 

  248. 248.

    Shai LJ, Masoko P, Mokgotho MP, Magano SR, Mogale AM, Boaduo N. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa. South Afri J Bot. 2010;76:6.

    Google Scholar 

  249. 249.

    Aderogba MA, Kgatle DT, McGaw LJ, Eloff JN. Isolation of antioxidant constituents from Combretum apiculatum subsp. Apiculatum. S Afr J Bot. 2012. doi:10.1016/j.sajb.2011.10.004.

    Google Scholar 

  250. 250.

    Jimoh FO, Adedapo AA, Aliero AA, Koduru S, Afolayan AJ. Evaluation of the Polyphenolic, Nutritive and Biological Activities of the Acetone, Methanol and W at er Extracts of Amaranthus asper. Open Complem Med J. 2010;2:7–14.

    Article  Google Scholar 

  251. 251.

    Magama S, Lieta MI, Asita AO. Antioxidant and free radical scavenging properties of four plant species used in traditional medicine in Lesotho. Int J Med Plants Res. 2013;2:170–8.

    Google Scholar 

  252. 252.

    Adedapo A, Jimoh F, Afolayan A. Comparison of the nutritive value and biological activities of the acetone, methanol and water extracts of the leaves of Bidens pilosa and Chenopodium album. Acta Pol Pharma Drug Res. 2011;68:83–92.

    Google Scholar 

  253. 253.

    Omoruyi BE, Bradley G, Afolayan AJ. Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province. BMC Complement Altern Med. 2012;12:215.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  254. 254.

    Adedapo AA, Jimoh FO, Afolayan AJ, Masika PJ. Antioxidant Properties of the Methanol Extracts of the Leaves and Stems of Celtis Africana. Rec Nat Prod. 2009;3:23–31.

    CAS  Google Scholar 

  255. 255.

    Wintola OA, Afolayan AJ. The antibacterial, phytochemicals and antioxidants Evaluation of the root extracts of Hydnora african at hunb. Used as antidysenteric in Eastern Cape Province, South Africa. BMC Complement Altern Med. 2015;15:307.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  256. 256.

    Adewusi EA, Afolayan AJ. Antibacterial, antifungal and antioxidant activity of the roots and leaves of Pelargonium reniforme Curtis (Geraniaceae). Afr J Biotechnol. 2009;8:6425–33.

    Article  Google Scholar 

  257. 257.

    Ntsoelinyane PH, Mashele SS, Manduna IT. The anticancer, antioxidant and phytochemical screening of Philenoptera violacea and Xanthocercis zambesiaca leaf, flower and twig extracts. Inter J Pharmacol Res. 2014;4:100.

    Google Scholar 

  258. 258.

    Nemudzivhadi, V., Masoko, P. (2014). In vitro Assessment of Cytotoxicity, Antioxidant, and Anti-Inflammatory Activities of Ricinus communis (Euphorbiaceae) Leaf Extracts. Evid. Based Complement. Altern. Med. 2014,

  259. 259.

    Jimoh FO, Adedapo AA, Aliero AA, Afolayan AJ. Polyphenolic Contents and Biological Activities of Rumex ecklonianus. Pharma Biol. 2008;46(5):333–40.

    CAS  Article  Google Scholar 

  260. 260.

    Oyedemi SO, Afolayan AJ. Antibacterial and antioxidant activities of hydroalcoholic stem bark extract of Schotia latifolia Jacq. Asian Pac J Trop Med. 2011;4:952–8.

    CAS  PubMed  Article  Google Scholar 

  261. 261.

    Jimoh FO, Adedapo AA, Afolayan AJ. Comparison of the nutritive value, antioxidant and antibacterial activities of Sonchus asper and Sonchus oleraceus. Rec Nat Prod. 2011;5:29–42.

    CAS  Google Scholar 

  262. 262.

    Oyedemi SO, Bradley G, Afolayan AJ. In -vitro and -vivo antioxidant activities of aqueous extract of Strychnos henningsii Gilg. Afri J Pharm Pharmacol. 2010;4:070–8.

    Google Scholar 

  263. 263.

    Olorunnisola OS, Bradley G, Afolayan AJ. Chemical composition, antioxidant activity and toxicity Evaluation of essential oil of Tulbaghia violacea Harv. J Med Plants Res. 2012;6:2340–7.

    CAS  Article  Google Scholar 

  264. 264.

    Noumedem JAK, Tamokou JD, Teke GN, Momo RCD, Kuete V, Kuiate JR. Phytochemical analysis, antimicrobial and radical-scavenging properties of Acalypha manniana leaves. SpringerPlus. 2013;2:503.

    PubMed  PubMed Central  Article  Google Scholar 

  265. 265.

    Ndjateu FST, Tsafack RBN, Nganou BK, Awouafack MD, Wabo HK, Tene M, et al. Antimicrobial and antioxidant activities of extracts and ten compounds from three Cameroonian medicinal plants: Dissotis perkinsiae (Melastomaceae), Adenocarpus mannii (Fabaceae) and Barteria fistulosa (Passifloraceae). S Afri J Bot. 2014;91(2014):37–42.

    CAS  Article  Google Scholar 

  266. 266.

    Kuete V, Azebaze AGB, Mbaveng AT, Nguemfo EL, Tshikalange ET, Chalard P, Nkengfack AE. Antioxidant, antitumor and antimicrobial activities of the crude extract and compounds of the root bark of Allanblackia floribunda. Pharma Biol. 2011;49(1):57–65. doi:10.3109/13880209.2010.494673.

    Article  Google Scholar 

  267. 267.

    Ngbolua, K.N., Mubindukila, R.E.N., Mpiana, P.T., Ashande, M.C., Baholy, R., F at iany, P.R., et. al. (2014). In vitro Assessment of antibacterial and antioxidant activities of a Congolese medicinal plant species Anthocleista schweinfurthii Gilg (Gentianaceae). J. Mod. Drug Disc. Drug Deliv. Res. I3,. DOI: 10.15297/JMDDR.V1I3.03.

  268. 268.

    Kuete V, Mbaveng AT, Maurice T, Benga VP, Etoa F, Nkengfack AE, et al. Antitumor, antioxidant and antimicrobial activities of Bersama engleriana (Melianthaceae). J Ethnopharmacolo. 2008;115:494–501.

    CAS  Article  Google Scholar 

  269. 269.

    Tagne RS, Telefo BP, Nyemb JN, Yemele DM, Njina SN, Goka SMC, et al. Anticancer and antioxidant activities of Methanol extracts and fractions of some Cameroonian medicinal plants. Asian Pac J Trop Med. 2014;7:442–7.

    Article  Google Scholar 

  270. 270.

    Fouedjou, R.T., Nguelefack-Mbuyo, E.P., Ponou, B.K., Nguelefack, T. B., Barboni L.,Tapondjou, L.A. (2016). Antioxidant Activities and chemical constituents of extracts from Cordyline fruticosa (L.) A. Chev. (Agavaceae) and Eriobotrya japonica (Thunb) Lindl, (Rosaceae). DOI: 10.5567/pharmacologia.2016.103.113

  271. 271.

    Soares MO, Rita C, Alves AC, Pedro C, Pires BDM, Beatriz PP, et al. Angolan Cymbopogon citratus used for therapeutic benefits: Nutritional composition and influence of solvents in phytochemicals content and antioxidant activity of leaf extracts. Food Chem Toxicol. 2013;60:413–8.

    CAS  PubMed  Article  Google Scholar 

  272. 272.

    Tamokou JD, Chouna JR, Fischer-Fodor E, Chereches G, Barbos O, Damian G, et al. Anticancer and Antimicrobial Activities of Some Antioxidant-Rich Cameroonian Medicinal Plants. Plos ONE. 2013;8:2.

    Article  CAS  Google Scholar 

  273. 273.

    Donfack JH, Nico FN, Ngameni B, Tchana A, Chuisseu PD, Finzi PV, et al. In vitro hepatoprotective and antioxidant activities of diprenylated isoflavonoids from Erythrina senegalensis (Fabaceae). Asian J Trad Med. 2008;5:172–8.

    Google Scholar 

  274. 274.

    Atsamo AD, Néné-Bi SA, Kouakou KL, Fofie KC, Nyadjeu P, Watcho P, et al. Cardiovascular and antioxidant effects of the methanol extract from the stem bark of Erythrina Senegalensis DC (Fabaceae). J Phys Pharm Adv. 2013;3:110–20.

    Article  Google Scholar 

  275. 275.

    Matsinkou RS, Ngondi JL, Kuate D, Mbofung C, Oben JE. Antioxidant and anti-hyperglycemic potential of pulp extracts of Irvingia wombolu fruits. Biol Med. 2012;4:10–9.

    Google Scholar 

  276. 276.

    Pieme CA, Ngoupayo J, Nkoulou CHK, Moukette BM, Nono BL, Moor VJA, et al. Syzyguim guineense Extracts Show Antioxidant Activities and Beneficial Activities on Oxidative Stress Induced by Ferric Chloride in the Liver Homogenate. Antioxidants. 2014;3:618–35.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  277. 277.

    Sishu R, Yonathan M, Seyoum A, Asres K. Radical Scavenging Activity of Volatile Oils Of Herbs Traditionaly Used To Spice Cooking Butter In Ethiopia. Ethiop pharm J. 2005;23:7–14.

    CAS  Google Scholar 

  278. 278.

    Tibuhwa DD. A Comparative Study of Antioxidant Activities between Fresh and Dry Mushrooms in the Genera Cantharellus and Afrocantharellus from Tanzania. Food Nut Sci. 2014;5:212–21.

    Article  Google Scholar 

  279. 279.

    Asamenew G, Bisrat D, Mazumder A, Asres K. In vitro Antimicrobial and Antioxidant Activities of Anthrone and Chromone from the Latex of Aloe harlana Reynolds. Phytother Res. 2011;25:1756–60.

    CAS  PubMed  Article  Google Scholar 

  280. 280.

    Asamenew G, Tadesse S, Asres K, Mazumder A, Bucar F. Study on the Composition, Antimicrobial and Antioxidant Activities of the Leaf Essential Oil of Apium leptophylum (Pers.) Benth. Growing in Ethiopia. Ethiop Pharm J. 2008;26:95–102.

    CAS  Google Scholar 

  281. 281.

    Burits M, Asres K, Bucar F. The Antioxidant Activity of the Essential Oils of Artemisia afra, Artemisia abyssinica and Juniperus procera. Phytother Res. 2001;15:103–8.

    CAS  PubMed  Article  Google Scholar 

  282. 282.

    Amabye TG, Bezabh AM, Mekonen F. Phytochemical Constituents and Antioxidant Activity of Delonix elata L. in Flower Extract. J Anal Pharm Res. 2016;2(1):00006. doi:10.15406/japlr.2016.02.00006.

    Google Scholar 

  283. 283.

    Onyancha JM, Cherongis CN, Nzivo JM, Muriithi GI, Njuguna DG, Mwalukumbi JM. Phytochemical screening and Evaluation of antioxidant activity of methanolic extract of Kenyan Hydnora abyssinica A. Braun (Hydnoraceae). J Innov Pharm Biol Sci. 2015;2:1–6.

    Google Scholar 

  284. 284.

    Vagionas K, Ngassapa O, Runyoro D, Graikou K, Gortzi O, Chinou I. Chemical analysis of edible aromatic plants growing in Tanzania. Food Chem. 2007;105:1711–7.

    CAS  Article  Google Scholar 

  285. 285.

    Asres F, Bucar F. Lippia adoensis var. adoensis: studies on the essential oil composition and antioxidant activity. Ethiop pharm j. 2002;20:31–5.

    CAS  Google Scholar 

  286. 286.

    Getahun Z, Asres K, Mazumder A, Bucar F. Essential Oil Composition, Antibacterial and Antioxidant Activities of Mentha aquatica Growing in Ethiopia. Ethiop Pharm J. 2008;26:9–16.

    CAS  Article  Google Scholar 

  287. 287.

    Karau GM, Njagi EM, Machocho AK, Wangai LN, Nthinga MJ. Chemical Composition and in vitro Antioxidant Activities of Ocimum americanum. Adv Anal Chem. 2015;5:42–9.

    CAS  Google Scholar 

  288. 288.

    Sishu R, Taddesse D, Bucar F, Asres K. Chemical composition and antioxidant activity of the essential oils of Ocimum americanum and Ocimum basillicum var. thyrsiflorum. Intern J Ess Oil Therape. 2010;4:64–8.

    CAS  Google Scholar 

  289. 289.

    Tadesse S, Asres K, Veeresham C. Antioxidant Activities of Three Rubus Species Growing in Ethiopia. Ethiop Pharm J. 2007;25:103–10.

    Google Scholar 

  290. 290.

    Asfaha H, Asres K, Mazumder A, Bucar F. Leaf Essential oils of Salvia nilotica and Salvia schimperi: Their Antimicrobial and Antioxidant Activities. Ethiop Pharm J. 2008;26:49–58.

    CAS  Google Scholar 

  291. 291.

    Wolde T, Engidawork E, Asres K, Eregete W. Evaluation of Hepatoprotective Activities of S at ureja punctata Benth Briq and Solanecio angulatus Vahl Jeffrey in ferric nitrillotriacetate induced hepatotoxicity in Rats. Ethiop Pharm J. 2010;28:63–74.

    Google Scholar 

  292. 292.

    Gebrelibanos M, Asres K, Veeresham C. In vitro Radical Scavenging Activity of the Leaf and Bark Extracts of Senna singueana (Del). Lock. Ethiop Pharm J. 2007;25:77–84.

    Google Scholar 

  293. 293.

    Chakraborty A, Asres K, Stipstis S, Eibl U, Brantnez AH. Biological Properties of Stephania abyssinica Roots. Pharmaceutical And Pharmacological Letters. 2000;10:19–21.

    CAS  Google Scholar 

  294. 294.

    Tabopda TK, Ngoupayo J, Liu J, Mitaine-Offer AC, Tanoli SA, Khan SN, et al. Bioactive aristolactams from Piper umbellatum. Phytochemistry. 2008;69:1726–31.

    CAS  PubMed  Article  Google Scholar 

  295. 295.

    Donfack JH, Njayou FN, Rodrigue TK, Chuisseu DDP, Tchana NA, Vita FP, et al. Study of a hepatoprotective and antioxidant fraction from Erythrina Senegalensis stem bark extract: in vitro and in vivo. Pharmacologyonline. 2008;1:120–30.

    Google Scholar 

  296. 296.

    Donfack JH, Wabo Fotso G, Ngameni CF, Tsofack ND, Ambassa B, Moundipa PF, et al. In vitro Hepatoprotective and antioxidant activities of the crude extract and Isolated compounds from Irvingia gabonensis. Asian J Trad Med. 2010;5:79–88.

    CAS  Google Scholar 

  297. 297.

    Cesari M, Hoerle C, Simoes-Pires P, Grisoli EF, Queiroz C, Dacarro L, Marcourt PF, et al. Anti-inflammatory, antimicrobial and antioxidant activities of Diospyros bipindensis (G urke) extracts and its main constituents. J Ethnopharmacol. 2013;146:264–70.

    CAS  PubMed  Article  Google Scholar 

  298. 298.

    Mohamed LE, El Nur EE, Choudhary MI, Khan SN. Bioactive Natural Products from Two Sudanese Medicinal Plants Diospyros mespiliformis and Croton zambesicus. Rec Nat Prod. 2009;3(4):198–203.

    CAS  Google Scholar 

  299. 299.

    Akimanya A, Midiwo JO, Matasyoh J, Okanga F, Masila VM, Walker L, et al. Two polymethoxylated flavonoids with antioxidant activities and a rearranged clerodane diterpenoid from the leaf exudates of Microglossa pyrifolia. Phytochem Lett. 2015;11:183–7.

    CAS  Article  Google Scholar 

  300. 300.

    Kouam SF, Ngadjui BT, Krohn K, Wafo P, Ajaz A, Choudhary MI. Prenylated anthronoid antioxidants from the stem bark of Harungana madagascariensis. Phytochemistry. 2005;66:1174–9.

    CAS  PubMed  Article  Google Scholar 

  301. 301.

    Waffo AFK, Mulholland D, Wansi JD, Mbaze LM, Powo R, Mpondo TN, et al. Afzeliixanthones A and B, two new prenylated xanthones from Garcinia afzelii Engl. (Guttiferae). Chem Pharm Bull. 2006;54:448–51.

    Article  Google Scholar 

  302. 302.

    Lannang AM, Komguem J, Ngninzeko FN, Tangmouo JG, Lontsi D, Ajaz A, et al. Bangangxanthone A and B, two xanthones from the stem bark of Garcinia polyantha Oliv. Phytochemistry. 2005;66:2351–5.

    CAS  PubMed  Article  Google Scholar 

  303. 303.

    Nzowa LK, Barboni L, Teponno RB, Ricciutelli M, Lupidi G, Quassinti L, et al. Rheediinosides A and B, two antiproliferative and antioxidant triterpene saponins from Entada rheedii. Phytochemistry. 2010;71:254–61.

    CAS  PubMed  Article  Google Scholar 

  304. 304.

    Kebede W, Bisrat D, Asres K. Free Radical Scavenging Activity-Guided Isolation of a Diterpenoid from Plectranthus punuctatus. Nat prod Comm. 2011;6:1229–32.

    CAS  Google Scholar 

  305. 305.

    Tangmouo JG, Raimana Ho R, Lannang AL, Komguem J, Lontsi TA, Lontsi D, et al. Norbergenin derivatives from the stem bark of Diospyros sanzaminika (Ebenaceae) and their radical scavenging activity. Phytochem Lett. 2009;2:192–5.

    CAS  Article  Google Scholar 

  306. 306.

    Bhebhe M, Muchuweti M, Chipurura B, Kasiyamhuru A. A comparative study of total phenolic content and antioxidant activity in Zimbabwean indigenous and exotic herbal teas. Biochem Pharmacol. 2013;2:4.–0501.S1.004.

    Google Scholar 

  307. 307.

    Muchuweti M, Nyamukonda L, Chagonda LS, Ndhlala AR, Mupure C, Benhura M. Total phenolic content and antioxidant activity in selected medicinal plants of Zimbabwe. Int Food Sci Technol. 2006;41:33–8.

    CAS  Article  Google Scholar 

  308. 308.

    Udu-Ibiam OE, Ogbu O, Ibiam UA, Nnachi AU, Agah MV, Ukaegbu CO, et al. Phytochemical and Antioxidant Analyses of Selected Edible Mushrooms, Ginger and Garlic from Ebonyi State, Nigeria. IOSR J Pharm Biol Sci. 2014;9:86–91.

    Google Scholar 

  309. 309.

    Adesuyi OA, Awosanya AO, Adaramola FB, Omeonu AI. Nutritional and Phytochemical Screening of Aloe barbadensis. Curr Res J Biol Sci. 2012;4:4–9.

    CAS  Google Scholar 

  310. 310.

    Kone, D., Diop, B., Diallo, D., Djilani, A., Dicko, D. (2012) IdentifiCAT ion, Quantit at ive Determin at ion, and Antioxidant Properties of Polyphenols of Some Malian Medicinal Plant Parts Used in Folk Medicine. InTech, Available from: quantitat ive-determination-and-antioxidant-properties-of-polyphenols-of-some-mali

  311. 311.

    Hamzah RU, Egwim EC, Kabiru AY, Muazu MB. Phytochemical and in vitro antioxidant properties of the Methanolic extract of fruits of Blighia sapida, Vitellaria paradoxa and Vitex doniana. Oxid Antioxid Med Sci. 2013;2:215–21.

    Google Scholar 

  312. 312.

    Iniaghe OM, Malomo SO, Adebayo JO. Hepatoprotective effect of the aqueous extract of leaves of Acalypha racemosa in carbon tetrachloride treated rats. J Med Plants Res. 2008;2:301–5.

    Google Scholar 

  313. 313.

    Ikewuchi JC, Uwakwe AA, Onyeike EN, Ikewuchi CC. Hepatoprotective effect of an aqueous extract of the leaves of Acalypha Wilkesiana ‘Godseffiana’ Muell Arg (Euphorbiaceae) against carbon tetrachloride induced liver injury in rats. EXCLI J. 2011;10:280–9.

    Google Scholar 

  314. 314.

    Nwozo SO, Oyinloye BE. Hepatoprotective effect of aqueous extract of Aframomum melegueta on ethanol-induced toxicity in rats. ACTA Biochemica Polonica. 2011;58:355–8.

    CAS  Google Scholar 

  315. 315.

    EL-Hawary S, EL-Shabrawy A, Ezzat S, EL-Shibany F. Gas chromatography–mass spectrometry analysis, hepatoprotective and antioxidant activities of the essential oils of four Libyan herbs. J Med Plants Res. 2013;7:1746–53.

    CAS  Google Scholar 

  316. 316.

    Ikyembe D, Pwavodi C, Agbon AN. Hepatoprotective effect of Methanolic leaf extract of anacardium occidentale (cashew) on carbon-tetrachloride-induced liver toxicity in wistar rats. Sub-Saharan Afr J Med. 2014;1:124–31.

    Article  Google Scholar 

  317. 317.

    Nasir A, Abubakar MG, Shehu RA, Aliyu U, Toge BK. Hepatoprotective Effect of the Aqueous Leaf Extract of Andrographis panicul at a Nees against Carbon Tetrachloride – Induced Hepatotoxicity in Rats. Nig J Basic Appl Sci. 2013;21:45–54.

    Google Scholar 

  318. 318.

    Oyedepo TA. Hepatoprotective Activity of Annona muricata leaf extract against paracetamol induced hepatotoxicity. In: A Quarterly Publication of the Faculty of Science. 1st ed. Ede, State of Osun: Adeleke University; 2014. p. 115–20.

    Google Scholar 

  319. 319.

    Johnson M, Olufunmilayo LA, Anthony DO, Olusoji EO. Hepatoprotective Effect of Ethanolic Leaf Extract of Vernonia amygdalina and Azadirachta indica against Acetaminophen-Induced Hepatotoxicity in Sprague–Dawley Male Albino Rats. Amer J Pharmacol Sci. 2015;3:79–86.

    Google Scholar 

  320. 320.

    Ojo OO, Nadro MS, Tella IO. Protection of rats by extracts of some common Nigerian trees against acetaminophen-induced Hep at otoxicity. Afr J Biotechnol. 2006;5:755–60.

    Google Scholar 

  321. 321.

    El-Beshbishy HA. Hepatoprotective Effect of Green Tea (Camellia sinensis) Extract against Tamoxifen-induced Liver Injury in Rats. J Biochem Mol Biol. 2005;38:563–70.

    CAS  PubMed  Google Scholar 

  322. 322.

    Awodele O, Yemitan O, Ise PU, Ikumawoyi VO. Modulatory potentials of aqueous leaf and unripe fruit extracts of Carica papaya Linn. (Caricaceae) against carbon tetrachloride and acetaminophen-induced hepatotoxicity in rats. J Intercult Ethnopharmacol. 2016;5:27–35.

    PubMed  PubMed Central  Article  Google Scholar 

  323. 323.

    Nadro MS, Onoagbe IO. Protective effects of aqueous and ethanolic extracts of the leaf of Cassia italica in CCl4–induced liver damage in rats. Amer J Res Comm. 2014;2:122–30.

    Google Scholar 

  324. 324.

    Uzzi HO, Grillo DB. The hepato-protective potentials of aqueous leaf extract of Cassia occidentalis against paracetamol induced hepatotoxicity in adult wistar rats. Inter J Herbs Pharmacol Res. 2013;2:6–13.

    Google Scholar 

  325. 325.

    Ottu OJ, Atawodi SE, Onyike E. Antioxidant, Hepatoprotective and hypolipidemic effects of methanolic root extract of Cassia singueana in rats following acute and chronic carbon tetrachloride intoxication. Asian Pac J Trop Med. 2013;6:609–15.

    CAS  PubMed  Article  Google Scholar 

  326. 326.

    Adebayo AH, Abolaji AO, Kela R. Hepatoprotective activity of Chrysophyllum albidum against carbon tetrachloride induced hepatic damage in rats. SENRA Acad Pub Bur Brit Col. 2011;5:1597–602.

    CAS  Google Scholar 

  327. 327.

    Ayakeme T, Essien ES, Akaninwor JO. Evaluation of hepatoprotective and in vivo Antioxidant Activity of the Methanolic stem extract of Costus afer (Bush Cane) in alcohol induced liver Cirrhosis in rats. J Biol Food Sci Res. 2014;3:29–34.

    Google Scholar 

  328. 328.

    Ezeonwu VU, Dahiru D. Protective Effect of bi-herbal formulation of Ocimum grat issimum and Gongronema latifolium Aqueous Leaf Extracts on Acetaminophen-induced Hep at o-Nephrotoxicity in Rats. Amer J Biochem. 2013;3:18–23.

    Google Scholar 

  329. 329.

    Adeneye AA, Olagunju JA, Elias SO, Olatunbosun DO, Mustafa AO, Adeshile OI, et al. Protective activities of the aqueous root extract of Harungana madagascariensis in acute and repeated acetaminophen hepatotoxic rats. Inter J Appl Res Nat Prod. 2008;1:29–42.

    Google Scholar 

  330. 330.

    Agbor GA, Oben JE, Nkegoum B, Takala JP, Ngogang JY. Hepatoprotective Activity of Hibiscus cannabinus (Linn.) Against carbon tetrachloride and paracetamol induced liver damage in rats. Pak J Biol Sci. 2005;8:1397–401.

    Article  Google Scholar 

  331. 331.

    Usoh IF, Itemobong S, Ekaidem OE, Etim E, Akpan HD, Akpan EJ, et al. Antioxidant and hepatoprotective effects of dried flower extracts of Hibiscus sabdariffa L. on rats treated with carbon tetrachloride. J Appl Pharm Sci. 2012;02:186–9.

    Google Scholar 

  332. 332.

    Famurewa AC, Kanu SC, Uzoegwu PN, Ogugua VN. Ameliorative effects of Hibiscus Sabdariffa extract against carbon tetrachloride-induced lipid peroxidation, oxidative stress and hepatic damage in Rats. J Pharm Biomed Sci. 2015;5:725–32.

    Google Scholar 

  333. 333.

    Omotayo MA, Ogundare OC, Longe AO, Adenekan S. Hepatoprotective effect of Mangifera-indica stem bark extracts on paracetamol-induced oxidative stress in albino rats. Eur Sci J. 2015;11:1857–7431.

    Google Scholar 

  334. 334.

    Effiong GS, Udoh IE, Udo NM, Asuquo EN, Wilson LA, Ntukidem IU, Nwoke IB. Assessment of Hepatoprotective and antioxidant activity of nauclea latifolia leaf extract against acetaminophen induced hepatotoxicity in rats. Inter Res J Plant Sci. 2013;4:55–63.

    Google Scholar 

  335. 335.

    Hassan SW, Salawu K, Ladan MJ, Hassan LG, Umar RA, Fatihu MY. Hepatoprotective, antioxidant and phytochemical properties of leaf extracts of Newbouldia Laevies. Inter J Pharm Tech Res. 2010;2:573–84.

    CAS  Google Scholar 

  336. 336.

    Udem S, Nwaogu I, Onyejekwe O. Evaluation of Hepatoprotective Activity of Aqeous Leaf Extract of Swietenia Mahogani (Maliaceae) in Chronic Alcohol-Induced Liver Injury in Rats. Maced J Med Sci. 2011;15:31–6.

    Google Scholar 

  337. 337.

    Danladi J, Abayomi KB, Mairiga AA, Dahiru AU. Comparative study of the hepatoprotective effect of ethanolic extract of Telfairia occidentalis (Ugu) leaves and silymarin on paracetamol induced liver damage in wistar rats. Inter J Anim Veter Adva. 2012;4:235–9.

    Google Scholar 

  338. 338.

    Minari JB. Hepatoprotective effect of methanolic extract of Vernonia amygdalina Leaf. J Nat Prod. 2012;5:188–92.

    Google Scholar 

  339. 339.

    Adewale OB, Adekeye AO, Akintayo CO, Onikanni A, Sabiu S. Carbon tetrachloride (CCl4)-induced hepatic damage in experimental Sprague Dawley rats: Antioxidant potential of Xylopia aethiopica. J Phytopharmacol. 2014;3:118–23.

    Google Scholar 

  340. 340.

    Sintayehu B, Bucar F, Veeresham C, Asres K. Hepatoprotective and Free Radical Scavenging Activities of Extracts and a Major Compound Isolated from the Leaves of Cineraria abyssinica Sch Bip exA Rich. Pharmacog J. 2012;4:40–6.

    Article  Google Scholar 

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Authors’ contributions

This work was carried out in collaboration between all authors. Author BL & OKS did the literature search. Author BL, OKS, FIO, EBB & MH carry out the data analysis and preparation of the manuscript. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

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Corresponding author

Correspondence to Bashir Lawal.

Additional file

Additional file 1:

Structure of chemical compounds isolated from African plants with potential antioxidants properties. (DOCX 121 kb)

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Lawal, B., Shittu, O.K., Oibiokpa, F.I. et al. African natural products with potential antioxidants and hepatoprotectives properties: a review. Clin Phytosci 2, 23 (2017).

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  • Antioxidants
  • DPPH
  • Hepatoprotective
  • Africa
  • Plants
  • Natural products